Table Of ContentVascular Endothelium
Physiological Basis of Clinical Problems"
NATO ASI Series
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Series A: Life Sciences
Vascular Endothelium
Physiological Basis of Clinical Problems II
Edited by
John D. Catravas
Medical College of Georgia
Augusta, Georgia
Alian D. Callow
Washington University Medical School
St. Louis, Missouri
and
Una S. Ryan
T Cell Sciences, Inc.
Cambridge, Massachusetts
Springer Science+Business Media, LLC
Proceedings of a NATO Advanced Study Institute on
Vascular Endothelium: Physiological Basis of Clinical Problems II,
held June 20-30, 1992,
in Rhodes, Greece
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Llbrary of Congress Cataloglng-ln-Publlcatlon Data
Vascular endothellum , physlologlcal basls of cllnlcal problems II I
[edlted by] John D. Catravas. AlIan D. Callow. and Una S. Ryan.
p. c •. -- (NATO ASI serles. Serles A. Llfe sclences ; v.
257)
"PrOceedlngs of a NATO Advanced Study Institute an Vascular
Endothellum, Physlolog1cal Basls of Cllnlcal Problems II. held June
20-30. 1992. In Rhodes. Greece"--T.p. verso.
"Publlshed In cooperat Ion w1th NATO Sclent1flc Affalrs Dlvlslon."
Includes b1b11ograph1cal references and index.
ISBN 978-1-4613-6035-3 ISBN 978-1-4615-2437-3 (eBook)
DOI 10.1007/978-1-4615-2437-3
1. Vascular endothelluN--Pathophyslology--Congresses.
1. Catravas. John D. II. Callow. AlIan D. III. Ryan. Una S .• 1941-
IV. North Atlantic Treaty Organizat Ion. Sclentlflc Affalrs
Dlvls10n. V. NATO Advanced Study Institute on Vascular Endothellum,
Physlolog1cal Basls of Cllnlcal ProbleNs II (1992 , Rhodes. Greece)
VI. Ser Ies.
[ONLM, 1. Endothellum. Vascular--physlopathology--congresses. as
532.5.E7 V3318 1992]
RCS91.4.V38 1993
S1S.1'307--dc20
ONLM/DLC 93-27610
for Llbrary of Congress CIP
ISBN 978-1-4613-6035-3
© 1993 Springer Science+Business Media New York
Originally published by Plenum Press, New York in 1993
Softcover reprint of the hardcover 1s t edition 1993
AII rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted in any
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otherwise, without writtl'ln permission from the Publisher
PREFACE
This book is a compilation of the lectures and oral and poster communications
presented at the Advanced Study. Institute on "Vascular Endothelium: Physiological
Basis of Clinical Problems II," which took place between June 20 and 30, 1992 in
Rhodes, Greece. This third in a series of ASIs on vascular endothelium continued on
the theme of the first (1988) ASI on "Receptors and Transduction Mechanisms" and
particularly expanded that of the 1990 conference on ''Physiological Basis of Clinical
Problems." We continued the successful practice of bringing together clinicians and
scientists: this was reflected equally well in the composition of the organizing
committee as in the background of the particiJ?ants. Endothelial cell functions and
dysfunctions present as many challenges to the mvestigator as they do to the curious
clinical practitioner. As these problems are necessarily different, this unique ten-day
co-habitation of these individuals continued to offer fresh outlooks to each, stimulated
potential collaborative efforts and, most importantly, advanced --ever so slightly--our
knowledge of vascular biology. This year's conference was further enriched by the
presence of several of our colleagues from Eastern Europe whom we are delighted to
welcome as officially sponsored participants to this and future NATO-supported
meetings.
It is never superfluous to remind readers and participants that those signing at
the bottom of this page, while responsible for many of the ASI's and the book's
deficiencies, are but three of the many contributors to the successes. We continue to
be impressed by the high and sustained interest and interaction of the participants,
reflected not only in the long formal--and after hours--discussion periods, but also on
their numerous and high quality presentations. Their enthusiasm and kind words of
praise are the major forces sustaining the continuation of these conferences. Similarly,
we are fortunate to depend upon individuals such as Norman Gillis, Alberto
Mantovani and Magdi Yacoub for their critical contributions in putting together a
stellar cast of lecturers and lectures that formed the nucleus of the ASI. We are
equally indebted to the local organizing committee of Panaviotis Behrakis, Michael
Maragoudakis and Lydia Argyropoulos for their tireless efforts before and during the
meeting. Similarly, we wish to thank Mary Snead, James Parkerson, Andreas
Papapetropoulos, Stelios Orfanos and Nandor Marczin for their considerable help in
the preparation of the conference. This year we were fortunate to have Mary Ann
Roupp responsible for both the processing of this book and the coordination of the
ASI. We wish to add to the praise offered by so many participants for the quiet,
efficient and friendly manner in which Ms. Roupp conducted the daily business of the
conference, as well as for the considerable work that she undertook for about a year
before and after the meeting. We are equally thankful for her exemplary editorial
work, which is evident in this book.
John Catravas (Augusta)
Allan Callow (St. Louis)
Una Ryan (St. Louis)
v
CONTENTS
Abbreviations . . . . . . • . . . . • . • . . . . . • . . . . . . . . . . . • . . . . . • . . • • . . . . . . .. ix
L FREE RADICALS/ISCHEMIA REPERFUSION INJURY
Basal Release of EDRF under Conditions of Oxidant Stress . . . • . . . . .. 3
Nandor Marczin, Xilin Chen and John D. Catravas
II. INVASION AND NEOPLASIA
Interaction of Malaria-Infected Cells with the Vascular Wall ......... 19
Giorgio Senaldi, Fabienne Taccbini-Cottier and
Georges E. Grau
Endothelial-Tumor Cell Interactions in Varo and in VIVO ••••••••••• 35
R. Giavazzi, A. Garofalo and I. Martin-Padura
III. ALTERED VASCULAR REACI'IVITY
Altered Vascular Reactivity in Coronary Artery Disease . . . . • . . . . . .. 45
Johannes Zanzinger and Eberhald Bassenge
Coronary Artery and Coronary Artery Bypass Graft Disease:
Role of the Endothelium and Vascular Smooth Muscle ..•........• 55
Thomas F.liischer
Isoforms of Nitric Oxide Synthase and the Nitric Oxide-Cycle
GMP Signal Transduction System ............................ 73
Ferid Murad, U. Forstermann, M. Nakane, J. Pollock,
H. Schmidt, T. Matsumoto, R. Tracey and W. Buechler
Regulation of Angiogenesis via Protein Kinase C ... . . . . . . • . . . • . .. 81
Michael E. Maragoudakis, Nikos E. Tsopanoglou and
George Haralabopoulos
IV. INFLAMMATION/ATIlEROSCLEROSIS
The Clinical Profile of Atherosclerosis . . . . . . . . . . . . • . . . . . • . . . . .. 89
Allan D. Callow
vii
Proinflammatory Role of Leukocyte Adhesion Molecules ........... 99
John M. Harlan, Robert K. Winn and Nicholas B. Vedder
Modulation of Endothelial Cell Function by Cytokines . . . . . . . . . . . .. 107
M. Introna, F. Breviario, E. d'A niello, E. Dejana and
Alberto Mantovani
Circulating Endothelial Adhesion Molecules . . . . . . . . . . . . . . . . . . . .. 115
Jolln L. Gordon, R.M. Edwards, SJ. Cashman,
AJ. Rees and AJ.H. Gearing
V. ORGAN TRANSPLANTATION/REJECTION
Experimental Cell Transplantation and Gene Therapy Techniques
in the Treatment of liver Disease ............................ 125
Achilles A. Demetriou, J. Roz~a, D. Neuzil, M. Holzman,
D. Griffin and Albert D. MosCloni
Modulators of Chronic Rejection ........•........ . . . . . . . . . . .. 135
Bengt Fellstrom and Erik Larsson
VI. EPILOGUE - 1992 NATO ASI Conference on
"Vascular Endothelium: Physiological Basis of Clinical Problems Ir ..... 147
John Gordon
VII. ABSTRAcrs OF ORAL AND POSTER PRESENTATIONS ..... . . .. 153
VIII. PARTICIPANTS .......................................... 219
IX. INDEX ................................................• 229
viii
ABBREVIATIONS
p-gal p-galactosidase
ACE angiotensin converting enzyme
ALB albumin
AMP adenosine 5' - monophosphate
ANKA
BA bovine aortic endot..lJelial cells
BPAE bovine pulmonary arterial endothelial cells
CAD coronary artery disease
CAM chorioallantoic membrane
CAPD chronic ambulatory peritoneal dialysis
cGMP cyclic guanosine 5'- monophosphate
CM cerebral malaria
CMV cytomegalovirus
cNOS constitutive nitric oxide synthase
CRP C-reactive protein
CSF colony - stimulating factor
crx cytotoxicity
CYSNO S-nitroso-L-cysteine
DG diacylglycerol
EC endothelial cells
ECE endothelin converting enzyme
EDNO endothelium-derived nitric oxide
EDRF endothelium derived relaxing factor
ELAM endothelial -leukocyte adhesion molecule
EUSA enzyme-linked immuno-absorbance assay
ET endothelin
FDD flow-dependent dilator response
G granulocyte
GAF guanylyl cyclase activating factor
GC guanylate cyclase
GM granulocyte-macrophage
GMP guanosine-5' -monophosphate
HAEC human aortic endothelial cells
HB hygromycin-B
HDL high density lipoproteins
HUVEC human umbilical vein endothelial cells
ffiMX isobutylmethyl xanthine
ICAM-l intercellular adhesion molecule
IL interleukin
IRF impaired renal function
ix
L-NAG L-nitro-arginine
L-NMMA L-mono-methyl-arginine
L-NNA L-amino-arginine
LDL low density lipoproteins
LNNA L-~-nitro-arginine
LPS lipopolysaccharide
LTBP latent TGF-binding protein
LTR long terminal repeat
M monocyte
MB methylene blue
MPANO S-nitrose-3-mercaptoproprionic acid
MW molecular weight
NANC nonadrenergic-noncholinergic
NAR nagase analbuminemic rats
NEM N-ethyl-maleimide
NO nitric oxide
ox-LDL oxidized low-density lipoproteins
PAEC pig aortic endothelial cells
PAF platelet activating factor
PCR polymerase chain reaction
PDGF platelet-derived growth factor
PKC protein kinase C
PMA phorbol 12-myristate 13-acetate, TPA
PRBC parasitized red blood cells
RBC red blood cells
RPASM rabbit pulmonary arterial smooth muscle
SAP serum amyloid P component
SLeX sialyl-Lewis'
SM smooth muscle
SMC smooth muscle cells
SNP sodium nitroprusside
SOD superoxide dismutase
TGF,Bl transforming growth factor,Bl
TNF tumor necrosis factor
TS thrombospondin
uv
ultraviolet
VCAM-l vascular cell adhesion molecule
VLA-4 very late antigen-4
WBC white blood cells
XO xanthine oxidase
x
I. FREE RADICALS/ISCHEMIA REPERFUSION INJURY
