Table Of ContentISSNNo.:0973-8045
The Pharmacist
M
Formulation
Biopharmaceutics
Pharmacokinetics
Pharmacology
Medicinal Chemistry
Analytical Chemistry
Herbal and Traditional Medicine
Biotechnology
VOLUME 2 NUMBER 2 2007
4-
.
THE PHARMACIST
AnInternationalBi-annualJournal.
Editor
Dr.A.Balasubramaniam.M.Pharm.,PhD.,Director,TIT-Pharmacy.Bhopal
AssociateEditor
Dr.B.K.Dubey M.Pharm.,PhD.,Director,TITCollegeofPharmacy,Bhopal
Dr.B.Sangameswaran.,M.Pharm.,Ph.D.,VinayakaMissionUniversity,Salem.
Dr.VimalKumarM.Pharm..PhD.,DirectorPCStudies.TITPharmacy,Bhopal
Mr.N.BalakrishnanM.Pharm., TITPharmacy.Bhopal
Mrs.B.ThirumagalMSc,M.R.Labs..Bhopal
Mr.C.S.SenthilKumar.MSc,M.Phil.,Biotech., M.R.Labs.,Bhopal
AdvisoryBoard
Dr.KatsumiKobayashi.Shizoka,Japan
Dr.K.SadasivanPillai,OrchidResearchlaboratoriesLtd.,Chennai
Dr.RameshNarayanan,GTXPharamceuticals,Memphis,U.S.A.
Dr.O.P.Katare,PunjabUniversity,Chandigarh
Dr.AnupamPathakM.Pharm.,Ph.D.,HODPharmacy,BarkatullahUniversity,Bhopal
Dr.B.JayakarM.Pharm.,Ph.D.,HODPharmacy,VinayakaMissionUniversity,Salem
Dr. Satish Nayak M.Pharm.,PhD.,DirectorBITS-Pharmacy,Bhopal
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ThePharmacist2(2)1-5(2007)
StudyofHepatoprotectiveActivityofPortulacaoleraceaL.
onRifampicinInducedHepatotoxicityinRat
A.S.kulkarni*,B.D.Siraskar,A.D.Dcshpandc,A.V.Kulkarni,
S.M.Dhonde,andBingi
DepartmentofPharmacology.Padam.Dr.D.Y.PatilInstituteofPharmaceuticalScienceand
Research.Pimpri,Pune411018.India.
ABSTRACT
EvaluationofhepatoprotectiveactivityagainstRifampicininducedhepatotoxicitiesinrat.of
hydroalcoholtcextractofthestemsandleavesofPortulacaoleraceaI. wascarriedout Thehydroalcoholic
extractofPortulacaoleraceaI.exhibitedsignificanthepatoprotectiveactivity(I' 0.01)closetosilymarin.a
wellknownashepatoprotectiveagent.
heywords:PortulacaoleraceaL.Rifampicin.hepatotoxicity.
INTRODUCTION Chemically,Portulacaoleraceacontainsmany
Liverplaysamajorroleindetoxificationandexcretion biologicallyactivecompounds,someofthembeing
ofmanyendogenousandexogenouscompounds,any nutritive.Someofthebiologically activecompounds
injurytoitorimpairmentsofitsfunctionmayleadto includealkaloids"omega-3 fattyacids,coumarins,
mdiasneyaseismpilsicsatitlilonascohnalolneen'gsehteoalathm.oMdaenrangememdeinctinoef.lTivheer tHaenpnaitnos,profltaevcotnioviedsa,ctciavridtiyacofglPyocrotsiudleasca.Tohleeraceamay
modernmedicineshavelittletoofferforalleviationof bemediatedbyitsantioxidantagentssuchasVitaminE,
hepaticailments,whereasmostimportantrepresentatives VitaminC,glutathione,P-caroteneandp-sitosterol.Acute
areofphytoconstituents. toxicitystudywascarriedoutonmicewereon2000mg/
Theencouragingresultsofthestudiesonthestems kgPortulacaoleraceawasfoundtobesafeand 1/10"1
and leavesofPortulacaoleracea L.(Ghole. fam. ofthatdoseconvertedforrat(70,140,280mgkg)and
Portulaceae) on preliminary basis for their wastakenforfurtherstudy.
(hceaprabtoonprotteetrcatcihvleoraicdtei)vitiynduacgaeidnshtepaatcoltaosxsiicciatlycihneimtiiactaeld MATERIALSANDMETHODS
ustoassesstheirhepatoprotectiveactivityagainstdrug Plantmaterial
inducedhepatotoxicity. ThefreshwholeplantsofPortulacaoleraceaL.
Portulacaoleraceahasbeenusedasfolkmedicine werecollectedduringthewinterfromafarminPune,
iInndtihfefeIrnednitacnousnutbrcioenstitnoetnrte,attdhieffpelraennttiasilrmeepnuttsedintohurmeadnusc.e IPnudniea..ITnhdeia,pl(avnotucshpeercismpeenciwmaesnanuot.hAenVtiKcaItIe)d.byB.S.I.
ulcers,tumors,inflammationandgoodforasthma,leprosy Preparationofthehydroalcoholicextractofportulaca
aaasacnnnnuddddrvaaapyslsitl;eaae\rsna.ttIoainnInvtitecci,i.hfsiuuInrnstageeiagdsilatariialndssneoldudisvuaeaesnrsdetiddcivinoisaroestalahliaseneadsgtgi,reednetpitaauu.trrlmyeImettnoisactnul,apsorporfyledefaermdtciiehgrsneeeetrraaaossnmeetisas,n ourfilsolieotnrmrgaTactht1eieeoa0mnp%d.leririvTnae/nhtdv.uearleqes.auoveleTosvhueeswneteprtoehwwaadensxoetlrrtahriceentsnei1d:de8ubveyraapwtcoaioorslsadtrfemoerdamc,o7e2vrhuearndt.d,ieobayrnt
bloodsugar,enhancesimmunity,andadjustsblood reducedpressure,inarotaryevaporatorat42-45"C.
lipidsA'.reviewofrecordsinscientificliteratureindicated ExpeArlilmeenxptearliamennitmaallprocedureswerecarriedoutinstrict
thatPortulacaoleraceahasmanymedicinal uses. accordancewiththeguidelinesprescribed by the
Reported pharmacological studies on Portulaca CommitteeforthePurposeofControlandSupervision
aomiculntseficvlrliaaetmc"ym*e,.aatnBoierrnuoycrn5loc,uphdrhoeeadlriamlaxaanatctnoiotbrlayoecgftifeeecfrcafitelacltoa4nc,tiinasvnkiaeatslylteg7ht,eamwslaiotcuaicnnaddnpadshtmeiaoaelnnoitttnish-g" oWCbneo)tmEwmaxeinpetdentrwei1eem8r.e0enttWaoaipts2pit0roa0onrvgeormdnatbAoyfn(iRetmiahtatehtleIusrnsss(teniCxtoPurwtvCiaeSogsnEiaculAstAeiRdsne.)igmT.wahelNeiogEa.thbh1ioi9nvc8ges'
antioxidantactivity'",antiulceractivity",andantitussuve animalsofeithersexwerepurchasedfromNational
effect1-'. ToxicologyCenter,Pune.Animalshadfreeaccessto
standardpelletdietandwateradlibitum.
*(orrespondingauthor-E-mail:ahijinkya6~ciyahoocom
Copyrightedmaterial
—
: Kuikarnielat.
Photomicrographofratliverofcontrol Photomicrographofratliverof
groupshowingnormalliver. Negativecontrolgroupshowing
HxEx400 portalinfiltrationalongwith
extensivedegenerationandnecrosis
ofthehepatocytesHxEx400
Photomicrographofratliverofsilymarin PhotomicrographofratliverofPO
treatedgroupshowingnoevidenceof 70treatedgroupshowingfew
necrosisandornecroticinfiltrate. regenerationchangeswithareasof
HxEx400. necrosisandinflammatoryinfiltrate.
HxEx400.
—K
[ '..r. . 3C amsp*
PhotomicrographofratliverofPOI40 PhotomicrographofratliverofPO
treatedgroupshowingnoevidence 280treatedgroupshowingnoevidence
ofnecrosisandclosetonormal. ofnecrosisandclosetonormal.
HxEx400. HxEx400.
Fig.IEffectofPOonhistopathologyofratliverinrifampicininducedhepatotoxicity.
HepatoprotectiveActivityofPortuIacaoleraceaL. 3
Table1
EffectofhydroalcoholicextractofPortulacaoleraceatreatmentondifferentbiochemicalparametersin
Rifampicininducedhepatotoxicity.
Serum Groups
Biochemical
|i.ir<imtitrs CNoonrtmraoll Nceognattriovle Pcoosnittriovle P7O0- P1O40- P2O80-
AST(U/ml) 46.58±2.44 122.26"±4.89 58.49±2.85 11417±4.49 104.12±3.26 63.21±3.06
ALI(U/ml) 40.25t2.24 95.84"±5.30 56.21±2.04 82.54*±3.67 74.58±3.47 69.54±2.85
AuLniPts/(dKl)A 13.54±1.22 52.49**±2.24 I7.87±1.67 48.52±2.04 36.89±1.55 23.54±1.I4
TB(mg/dl) 0.58±0.020 1.56""±0.061 0.86±0.032 1.36^0.057 1.02±0.048 0.94±0.036
TP(gm/dl) 10.14±0.83 5.47"*±0.41 9.08±0.67 5.01±0.53 8.01±0.39 8.82±0.40
n<D=u06n..n0eV1t,atlwuheestnesatrc.eomepxparreesdsewditahsNMoeramnal±cSo.nEt.roMl."S=tapti<sti0c.a0l1lywahneanlyczeodmpbayreOdnewiWthayNeAgaNtOivVeAcofnotlrloolwe"d=byp
PO-Portulacaoleracea
TreatmentScheduleForRifampicinInduced GroupV:Hydroalcoholicextractof(Portulacaoleracea
Hepatotoxicity14 -140)-Theanimalsinthisgroupreceivedrifampicin
agvwCnererhioroimuecaupllpedsr.oeiIn:cnee4Nivooednrdimvaiadfidlstetedirclodl4neotd8sreowshl.ar.tTeCrharfeot(uelbrpmi-loo/cvThIeehOrmOenigicmagan,hlitmpeaefslratssiotmriiaanntlgi)tohonaissfs (To(mvIihIeg4nerm0.n/mbikgigg/ohh.ckythgdpe,rfemaoripsa.cotlorai.canl)loghewioosnaftls5iiamc%naaidtgemmixaoiutlnnmrssia.sacwtcteearrcoeeifda)dPioanontrefdtoaauuyfrltaeIdcrsi'ava4if8tdoeelhrdre.trdhaaoefcste3ee0a.r
gadGcroraonocueiupapa)frteIaeIctr:ei4dNv8aeeygdharto.rniiaevff.eatmeCpTriohconeivtnerbroi(nlIoiggcChmhrt/eokmfugia,pcsa-tplienTrgheseootrfiaamlnaaintiminimaoal5lnsss%.inwgetuhrimes CoaorfillrtfeeeoarrrmauapctcpiehecaeaiVn(3I-20(:28I80gm0Hmmi)gy/n/k.d-kgrg,To,hhapyeledprceroaroonrhaaoilolrcmlaoaliih)nlcos5lwiea%icxnstgertauxhadtmicmrstiaancgcitoarscfotoiuefa(rp)PePoadortrretditcnuauelylifaavoIcceu"adar,
GroupIII:PositivecontrolGroup-Theanimalsinthis divideddose.Thebiochemicalestimationsweredone
groupreceivedrifampicin(Igm/kg,peroralin5%gum after48hr.afterovernightfastingofanimals.
acacia)atdayoneafterthe30min.Silymarin(100mg/kg, Biochemicalestimations
opbveierorcnohireagmlh)itcwafalassteisantdgimmioanftiaisnotinemsraeldwse.irnefdoournediavfitdeerd4d8osher..aTftheer wanaismaOwlnist2whneddrreadwaanyneasbftytheertpiouzvneecdrtuwnriitighnhtganfreaesstttrhioen-tgoirocbfietatahnleirmpaallnsedxaublsllotbohdye
GroupIV:Hydroalcoholicextractof{Portulacaoleracea usingfineglasscapillarytubeandcollectedinplain
-kg7.0)p-erThorealaniinm5al%sginutmhiascgarcioau)partecdeaiyveId"raiftfearmptihcein30(Imgimn.' ssteeprairlaetceedntbryifcuegnetritfuubgeastiaonndatal7l0o0w0edrptmocfloort.15Semriun.mawta5s"
hydroalcoholicextractofPortulacaoleracea(70mg'kg. C.TheseparatedserumwasusedforestimationofAST,
peroral)wasadministeredinfourdivideddose.The ALT,ALP,TPandTB.
obvieorcnhiegmhitcaflasteisntgimoaftianoinmsalwse.redoneafter48hr.after HistOopnat2hnodlodgaiycatlheiannviemsatlisgawteironessacrificedandabdomen
Copyrightedmaterial
.
4 Kulkarnictal.
wascutopen,theliverwasdissectedoutandstoredin andregenerativeactivity;henceitcouldbeuseinthe
10%formalinsolution.Theliverwasprocessedforthe treatmentofliverdisorderslikeliverdysfunction,viral
histopathologicalinvestigations. hepatitisandvariousalcoholicliverdisorders.
Statisticalanalysis:
Tanhaelyrzeesdultbsyaornee-ewxapryesAsNedOVasAmfeoallnow±edS.bEy.MD.unDnaetttatweasts. REFERENCES
Valueofplessthan5%(i.e.p-0.05)wasconsidered
statisticallysignificant. 1 NadkarniK.M.,IndianMateriaMedica,.3"1ed.
Mumbai:PopularPrakashan;2000.p.1005-1006
RESULTSANDDISCUSSION
:
Rifampicin.abroadspectrumantituberculardrugs, 2 RasheedA.N.,AfifiF.U.,DisiA.M.,Simpleevaluation
ishepatotoxiconchronicusage.Itismetabolisedtoan ofthewoundhealingactivityofacrudeextractof
activemetabolite.25-desacetylrifampicin.Itreducesdrug PortulacaoleraceaL.inMusmusculusJVI-1,
metabolisingenzymesandactivelyandspecificallybinds JournalofEthnopharmacology,2003;88:131-136.
toRNApolymeasesandtherebyinhibitsthesynthesis
ofallformsofRNAThus,byinhibitingnucleicacidand 3 SunJ.,GuoB..ZangH.,ZangJ.,ZangT.Chemical
proteinsynthesis itinduces fattyliverand finally compositionfromPortulacaoleraceaL.,Journalof.
cirrhosis.Theratsintoxicatedwithrifampicinelevated ChinesePharmaceuticalSciences,2004;4:150-157.
signiIfnictahnetlypr(eps<e0n.t01st)utdhye,stehreumASbTio,chAeLmiTcallevpealraimnectreerasse1'd. 4 Zhang,X.J.,Ji.Y.B.,Qii,Zh.Y..Xia,J.Ch.,Wang,L.,
byrifampicintreatmentwassignificantly(p<0.0l)reduced Experimentalstudieson antibioticfunctionsof
byhydroalcoholicextractofPortulacaoleracea(140 PortulacaoleraceaL.invitro,ChineseJMicroecol.
and280mg/kg)after48hr. 2002;14(5):277-280.
ALPactivity is related to functioningofthe
hepatocytecells.ThisALPlevelwasreducedsignificantly 5 Chan,K.,Islam,M.W.,Kamil,M.,Radhakrishnan,
byhydroalcoholicextractofPortulacaoleracea(140 R..Zakaria,M.N.M.Habibullah,M.andAttas,A.,
and280mg/kg)(p<0.01)after48hr. Theanalgesicandanti-inflammatoryeffectsof
Thetotalbilirubinandtotalproteinlevelwasalso PortulacaoleraceaL.subsp.Sativa(Haw.)Celak.J.
restoredtothenormallevelbythehydroalcoholicextract Ethnopharmacol.,2000,(73):445-451
ofPortulacaoleracea(140and280mg/kg)(p<0.01)after 6 OkwuasabaF.,EjikeC,ParryO.,Effectofan
48hr. aqueousextractofPortulacaOleraceaLinn,leaves
TheserumlevelofbiomarkersinPortulacaoleracea onsmoothmuscleandratbloodpressure.Journal
(140and280mg/kg)treatedgroupwasrestoredtonormal of.Ethnopharmacology,1988;22:33-44.
level. The result indicates that treatment with 7 RadhakrishnanR.,AttasA.,ChanK.,ChenH.B.,
hydroalcoholicextractofPortulacaoleracea(140and IslamM.W.,ZakariaMNM,Neuropharmacological
280mg/kg)protectsliveragainstrifampicininduced actionsofPortulacaoleraceaL.subsp.Sativa
hepatotoxicity. (Havvk.),JournalofEthnopharmacologv.76;2001:
Thehistopathologyofthelivergivestheevidence 171-176.
fortheprotectionimpartedbythehydroalcoholicextract 8 Rasheed,A.N.,Afifi,F.U.andDisi,A.M„Simple
andSilymarin.Controlgroupshowsnormalhepaticcells. evaluationofthewoundhealingactivityofacrude
Whereasadministrationofrifampicininnegativecontrol extractofPortulacaoleraceaL.inMusmusculus
groupshowedportalinfiltrationalongwithextensive JVI-1.J.Ethnopharmacology.,2003,88:131-136.
degenerationandnecrosis.TreatmentwithSilymarin 9 MalekF.,BoroushakiM.T.,BoskabadyM.H.,Tohidi
showednormalhistologicalappearancewithnosignof M.,Bronchodilatoryeffectof Portulacaoleracea
necrosis.WhereasPortulacaoleracea 140and280 L.inairwaysofasthmaticpatients.Journalof
treatedgroupshowedclosetonormal.Theoverall Ethnopharmacology..2004,93:57-62.
structurewasnormal. 10 TowatanaN.H.,ChaiyamuttiP.,MahabusarakamW.,
The Hepatoprotective activity of Portulaca PanthongK.andRukachaisirikulV,Antioxidative
oleraceamaybemediatedbyitsantioxidantsthatit andfreeradicalscavengingactivitiesofsomeplants
containssuchasVitaminE,VitaminC,Flavonoids,p- usedinThaifolkmedicine.PharmaceuticalBiology,
carotene,P-sitosteroIandomega-3fattyacid. 2006,44(3):221-228.
CONCLUSION 11 KarimiG.,EttehadN.,HosseinzadehH..Evaluation
thhyedrTopaarlkecsioenhngotliiinctnoveexcstotrniasgciatdtiehoranas,tiaoitndectfahinneitbreeeshucleotpsnatcoolbputrdaoeitdnecetdtihvaietn oo2lf00et4rh.aec1e8ga(a6s)Lt:.ri4ce8x4ta-rn4at8ci7tus.lcienromgiecnei,cPehfyfetcotthoefrpPyorRteuslearccah.,
Copyrightedmaterial
HepaloprotecliveActivityofPortulacaOleraceaL. 5
12 BoroushakiM.T.,BoskabadyM.H..MalckI. 14 BafnaA.R.,MishraS.H..Effectofmethanolic
Antitussiveeffectoff'ortulacaoleraceaL.inguinea extractofAchyranthesasperaLinn,onrifampicin
pigs,IranianJournalofPharmaceuticalResearch, inducedhepatotoxicityinrats,Ars.Pharm.2004,
2004;3:187-190. 45:(4):343-351.
13 XiangL..DingY,DuL,WangR,WangW.andXing 15 BowmanW.C.,RandM.J..Textbookof
D.,AlkaloidsfromPortulacaoleraceaL.,Journalof pharmacology,Blackwellscientificpublication,
Kthnopharmacology.2005.66:57-62. OxfordLondon,1982;2~,ed.p.34-39.
Copyrightedmaterial
ThePharmacist2(2)7-10(2007)
InvitroScreeningofZiziphusjujubafortheirAntioxidantand
Anti-adipogenicactivity
A.R.Srividya* P.HariBalaji,II.RaghuandP.Vijayan
DepartmentofPharmaceuticalBiotechnology,J.S.S.CollegeofPharmacy,Ooty-643001
ABSTRACT
oafntlieoaxviedsanotIfnacZttihizeviippthyruewssiejtnhutjEuwboarwk,hvaasvlaurebieooeufns2c.sa5tr1udrii±eesd0.so5uu0ct3haZsizaminlpthiouExstihjdaaunnjotul,bicacyetxoltteroaaxfcitce,xotfraanZctitizmieipxchhruiosbbiijtaueljdu,abpcatoitvleientatyf
showedgoodcytotoxicactivityagainstnormal,cancerandadipocytecellline. Thisleafextract
showedmoderateinhibitoryactivityagainsttheselectedmicroorganisms TheMICvalueswere
foundtobebetween500 1000',-4gml
Keywords Antioxidant,antimicrobial,anti-adipogenic.Cytotoxicity.
INTRODUCTION AandjujuninsB,vitaminC. Seedscontainspinosin,
Zizipusjujuba plantwascollectedfrom theroad swertisin,sinapolyspinosin, p-coumaloylspinosin,
sidesin Erode inthemonthofMay2005. Itwas glycosylisoswertisin,vicenin-2flavanoid-apigenin
aMuetdhiecnitniaclatpeldanbtysDsru.rvSe.yRaanjdan,colBloetcatniiocnalunistu.rvGeoyveorfnImnedinat, MATERIALSANDMETHODS
ArtsCollege.Ootacamund.Tamilnadu,India. Ziziphus Thecollected leaves wereseparated fromtherest
jcCturuhejliatuntiabv.ehateAeabuddsetalirconahnldegirasybaitnlloadinotdAuhsfsernieicnsfasa..ImnidTilrTaya,hdeiRtshibroaainmrlaaknlnlakyicas,eraouAosefte.gdh'isant:uoissetItdtraentai,ots wpwmaaeercrrteeeroafectxotiathorrensaecplmltyaeendtpthosoweaddpn.eadrreasTdthhe.aeldyeTehxudtesrriiapencdogt.wsd5Twe0her%ereendetcltheoheanavcndeeorsnlitemrdabatytelerecdiaoavlletdsso
buosields,asulacsetrrs,ingdeynstenitnergyingainvidtidsi.arrThheea.leTahveesbaarrekuissedalstoo dtermypneersastuurend'erThreedreuscueltdspwreersesutraebulaantdedtihneTacbolnetr-ollI.ed
treatbiliousness,diarrheaandantipyretic,reduces Forthisleafextract phytochemicalconstituentssuchas
obesity,scabiesandotherskindiseases. Thesmoke totalphenoliccontent",totalflavonolcontent''7were
aafpnrhodrmocdtoihusegidahrc.i,edtTolhneeaivcersliapixesatifinrvhueai,tlediisnfvocirogootlrhiaenttigrn,egaitanmndeidngtersoetfimcboolvleed, eDmsePttiPhmHoatdemd"e..tHhyAodndrtoi\xoyxlNiidtraranidtcicoaslxtuisddcieeasvreawndeigcriaenlgaialncshtoiivbpiiettyriofbnoyramPcetNdivDibtAyy
catbbosoilnnloisiloctoad,iuxpasaaatnpntiehdivsreosoandlaihlansaandiyvsdaicant,bphglipcreosustoteri.dnezseTaerdthysiie,esoendsar,iseeseemetsdhao.sisvrease,Ttrhecievomaocpudmrugriirhydtitiaafinrsnegutdsiht,msfawagrre,ooeeomttudhiisstrehhsid,etn (strHphacyde-adivclrnaeeoilantgfgbrewiyonensrgaoelpdkmeiaaerlmltsoieonhxteiohsddycDelrMeneraSnanaeOdindildcimfaneolesrt)chta11homvedeeiArnt"BghC.iyToFnt.dSogthtao"orn'xf,oaildcSsiiicucctpayaelevxbrteyrcnaoagMcxttiiiTndooTgnef
"vata"burning,J Assaymethod14andsulphordamineB(SRB)Method'\
Thisplantcontainsfragufolinealkaloid:Matiritines Theextractsoftheleaveswerescreened fortheir
A-H. saponninsjujubosideA,B;alkaloidsnummulurine antimicrobialactivityagainsttheselectedmicroorganism
D-H,K;amphibinesB.DandFhyodricanineAjubanine bytwofolddilutionmethodtodeterminetheminimum
AjubanineB:yuziphines. Barkandrootcontains inhibitoryconcentration"'.lixtractsweretestedfortheir
triterpenoidzizyberanelicacid. Barkcontainalkaloids- anti adipogenicactivity in373- l.l cell line.The
MauritinesA-F:amphibinesB-DandP.Frangufolin.Stem concentrationoftheextractchosenforthetestingwere
ccdfryoeucnmilttmoaJapciureonjapnuttnbsaieeaid.spneaospgasaloetpknenoapiinlhnnoias,innd,iszIn,-ifezl.fIaoIrIvgNa.ae-nnnJgoiuuninfjdo.ousrl-bmiLonzuegeciaeizvnaifleipeknsrhaiilnngcoelo,iydncsatosa-isimijnidubejRoduul.ntiiinnneAs., dewmbseeeatlasxiosamwuimanrtedetethuddehciaeaurtdzsCio4b'nn9yIge0CVIoniaBlmnMvdraXeldiu(neO3s-ualsniitsdnao.intbiwuntoTgyh.ldeilITu-fhtaeiMtoenatf.bhosyAroldmriabxptaaoinngotcenhenaenwwseaai)ssS.
'Correspondingauthor-ll-mad:pharmarsrividyaayahoo.com
Copyrightedmaterial
!
Srividyaelal.
Table1
PercentageofYieldofTheLthanolicExtractofZiziphusJujuba
Nairnoftheplant Partoftheplant weightofthe weightofthe Percentageofyield
Sample(g) crudeextract
Obtained(g)
Xiziphus
jujuba leaves 225 32.95 14.6%
Table2
Phyto-ChemicalConstituentsofZiziplmsJujubaLeaves
Test ZiziplmsjujubaLeaves
Glycosides +
Tannins
Saponines +
Flavonoids +
Steroids
Terpenoids
Carbohydrates
Protein
TotalPhenoliccontentin 51.35
Percentage
Totalflavonolcontentmg/g 7.7
Table3
AntioxidantActivitybyVariousMethodsforZiziphusJujuba
Methods ICjoug'ml
FortheZiziphusjujubeleaves
DPPIImethod:
Standard: 30.5±5.26
Ascorbicacid 2.69±0.05
Rutin 3.91±0.1
NitricoxideMethod
Standard: 365±10.52
Rutin 68.44±2.56
PNDAmethod
Standard: >I000
Ascorbicacid >1000
Rutin >I000
Hydrogenperoxidemethod
Standard: >1000
Ascorbicacid 187.33±3.93
Rutin 36.16±0.16
ABISmethod
Standard: 2.51±0.503
Ascorbicacid 11.25±0.49
Rutin 0.51±0.01
AlkalineDMSOMethod
Standard: >I000
Ascorbicacid >1000
Rutin >1000
Totalantioxidantactivity
Phosphomolybdenum 0.96±0.03
Method.
