Table Of ContentStandard Operational Procedures in
Reproductive Medicine
Laboratory and Clinical Practice
REPRODUCTIVE MEDICINE AND ASSISTED REPRODUCTIVE
TECHNIQUES SERIES
David Gardner
University of Melbourne, Australia
Zeev Shoham
Kaplan Hospital, Rehovot, Israel
Kay Elder, Jacques Cohen
Human Preimplantation Embryo Selection, ISBN: 9780415399739
John D Aplin, Asgerally T Fazleabas, Stanley R Glasser, Linda C Giudice
The Endometrium, Second Edition, ISBN: 9780415385831
Nick Macklon, Ian Greer, Eric Steegers
Textbook of Periconceptional Medicine, ISBN: 9780415458924
Andrea Borini, Giovanni Coticchio
Preservation of Human Oocytes, ISBN: 9780415476799
Steven R Bayer, Michael M Alper, Alan S Penzias
The Boston IVF Handbook of Infertility, Third Edition, ISBN: 9781841848105
Ben Cohlen, Willem Ombelet
Intra-Uterine Insemination: Evidence Based Guidelines for Daily Practice,
ISBN: 9781841849881
Adam H. Balen
Infertility in Practice, Fourth Edition, ISBN: 9781841848495
Nick Macklon
IVF in the Medically Complicated Patient, Second Edition:
A Guide to Management, ISBN: 9781482206692
Michael Tucker, Juergen Liebermann
Vitrification in Assisted Reproduction, ISBN: 9780415408820
Ben J Cohlen, Evert J P van Santbrink, Joop S E Laven
Ovulation Induction: Evidence Based Guidelines for Daily Practice,
ISBN: 9781498704076
Botros Rizk, Markus Montag
Standard Operational Procedures in Reproductive Medicine:
Laboratory and Clinical Practice,
ISBN: 9781498719216
Standard Operational Procedures in
Reproductive Medicine
Laboratory and Clinical Practice
Edited by
Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS
Professor and Head of Reproductive Endocrinology and Infertility
Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction
University of South Alabama
Mobile, Alabama, USA
Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility
Obstetrics and Gynecology
King Abdulaziz University
Jeddah, Kingdom of Saudi Arabia
Distinguished Adjunct Scientific Director
IVF Michigan
Rochester Hills, Michigan, USA
Markus Montag, PhD
Professor and CEO
ilabcomm GmbH
Sankt Augustin, NRW, Germany
CRC Press
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© 2017 by Taylor & Francis Group, LLC
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International Standard Book Number-13: 978-1-4987-1921-6 (Paperback)
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To my father, Mitry Botros Rizk, 1916–2003, a great and most caring man
who inspired me every day of my life and lived to do that.
Contents
About the Editors .....................................................................................................................................................................xi
List of Contributors ................................................................................................................................................................xiii
Section I Laboratory Procedures
1. The Documentation System .............................................................................................................................................2
Markus Montag and Lars Johansson
2. Culture Media Techniques ..............................................................................................................................................4
Lars Johansson
3. IVF Incubator Handling .................................................................................................................................................8
Jason E. Swain and Alex Lagunov
4. Preparation for Follicle Aspiration and Isolation of Cumulus-Oocyte-Complexes (COC) ....................................12
Markus Montag
5. Semen Collection and Sample Reception .....................................................................................................................14
Carin Huyser
6. Spermiogramm, Makler Chamber, Neubauer Chamber, CASA ..............................................................................18
Verena Nordhoff
7. Sperm Viability Testing .................................................................................................................................................20
Markus Montag
8. Sperm Preparation—Swim Up, Density Gradient, Migration Chamber, Zeta Method .........................................22
Thomas Ebner
9. Sperm Selection Using Hyaluronan Binding ...............................................................................................................24
Denny Sakkas
10. IVF—Insemination.........................................................................................................................................................26
Markus Montag
11. ICSI ..................................................................................................................................................................................28
Thomas Freour
12. Oocyte In Vitro Maturation—Isolation of COCs and Culture for Maturation .......................................................30
Mariabeatrice Dal Canto and Giovanni Coticchio
13. Artificial Oocyte Activation ..........................................................................................................................................32
Markus Montag
14. Cryopreservation—Sperm TESE and MESA .............................................................................................................34
Borut Kovačič
15. Vitrification—Oocytes, 2PN, Embryos, Blastocysts ...................................................................................................36
Ana Cobo and Aila Coello
viii Contents
16. Slow Freezing: Principles, Oocytes, 2PN, Embryos, Blastocysts ..............................................................................38
Etienne Van den Abbeel
17. Oocyte Selection .............................................................................................................................................................40
Basak Balaban
18. Zygote Selection ..............................................................................................................................................................42
Martin Greuner
19. Selection and Grading of Embryos on Day 2/3 and Blastocysts on Day 5/6 .............................................................44
Thorir Hardarson and Julius Hreinsson
20. Time-Lapse Imaging ......................................................................................................................................................46
Cristina Hickman
21. Assisted Hatching ...........................................................................................................................................................50
Christian De Geyter and Maria De Geyter
22. Zona Manipulation at Various Embryo Stages: Mechanical/Acidic Medium .........................................................52
Marius Meintjes
23. Biopsy: Polar Body, Blastomere, Trophectoderm .......................................................................................................56
Hakan Yelke and Semra Kahraman
24. Sampling of Cells after Biopsy for aCGH ....................................................................................................................60
Barry Behr
25. Embryo Transfer—Standard and Ultrasound Guided ..............................................................................................62
Bettina Toth
26. Hygienic Standards in the IVF Laboratory ................................................................................................................64
Vera Baukloh
27. Quality Control of IVF Laboratory Supplies—Mouse Embryo Assay (MEA) ........................................................68
Dean E. Morbeck
28. Troubleshooting in the IVF Laboratory ......................................................................................................................70
David Mortimer and Sharon T. Mortimer
29. Microscopy ......................................................................................................................................................................72
Markus Montag
30. Fertility Preservation .....................................................................................................................................................74
Markus Montag and Jana Liebenthron
Section II Clinical Procedures
31. Ultrasound Evaluation in Treatment Management ....................................................................................................78
Ippokratis Sarris and Geeta Nargund
32. Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies ...............................................................80
Magued Adel Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk
33. Infertility Ultrasound Evaluation: One-Stop Procedure ...........................................................................................82
Spiros A. Liatsikos and Geeta Nargund
Contents ix
34. Office Hysteroscopy ........................................................................................................................................................86
Linda D. Bradley
35. Hysteroscopic Management of Uterine Septum ..........................................................................................................88
Omar Abuzeid and Mostafa Abuzeid
36. Hysteroscopic Lysis of Intrauterine Adhesions ...........................................................................................................92
Bolonduro Oluwamuyiwa and Mostafa Abuzeid
37. Hysteroscopic Management of Submucosal Fibroids .................................................................................................94
Salem Joseph and Mostafa Abuzeid
38. Saline Infusion Sonohysterography ..............................................................................................................................98
Alan Bolnick and Mostafa Abuzeid
39. General Principles of Laparoscopy ............................................................................................................................100
Rubin Raju and Mostafa Abuzeid
40. Microlaparoscopy .........................................................................................................................................................104
Oscar D. Almeida, Jr.
41. Laparoscopic Myomectomy and Mini-Laparotomy: An Alternative Approach for Myomectomy .....................106
Ahmed Abdelaziz and Mostafa Abuzeid
42. Laparoscopic Excision of a Large Endometrioma ....................................................................................................108
Rubin Raju and Mostafa Abuzeid
43. Laparoscopic Management of Subtle Fimbrial Pathology Secondary to Endometriosis .....................................110
Omar Abuzeid and Mostafa Abuzeid
44. Laparoscopic Management of Mature Cystic Teratoma..........................................................................................112
Rahima Sanya and Mostafa Abuzeid
45. Tubal Cannulation for Proximal Tubal Disease ........................................................................................................114
Wael Salem, Osama A.H. Abu Zinadah, Joshua Ekladios, and Botros Rizk
46. Robotic Assisted Laparoscopic Myomectomy ...........................................................................................................116
Hashem Lotfy, Arsany Bassily, Lauren Mann, and Botros Rizk
47. Robotic Assisted Tubal Anastomosis after Tubal Sterilization ...............................................................................118
Hashem Lotfy, Rola Turki, and Botros Rizk
48. Unexplained Infertility ................................................................................................................................................120
Hassan N. Sallam
49. Male Evaluation for Infertility ....................................................................................................................................122
Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal
50. Hysterosalpingography ................................................................................................................................................124
Islam Fahmi, Mostafa Abuzeid, and Shawky Z.A. Badawy
51. Intrauterine Insemination: Clinical Perspectives .....................................................................................................128
Willem Ombelet
52. GnRH Agonist to Trigger Final Follicular Maturation in IVF...............................................................................132
Nayana Talukdar and Robert F. Casper
