Silica-based Antibacterial and Antifungal Nanoformulation DIV

21 Pages·2016·1 MB·English

by  Swadeshmukul Santra

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UCF Patents Technology Transfer
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Swadeshmukul Santra
University of Central Florida
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Santra, Swadeshmukul, "Silica-based Antibacterial and Antifungal Nanoformulation DIV" (2014). UCF
Patents. 515.
https://stars.library.ucf.edu/patents/515
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c12) United States Patent (10) Patent No.: US 8,632,811 Bl
San tr a (45) Date of Patent: Jan.21,2014
(54) SILICA-BASED ANTIBACTERIAL AND Yeshchenko, Oleg, Influence of Annealing Conditions on Structure
ANTIFUNGAL NANOFORMULATION and Optical Properties of Copper Nanoparticles Embedded in Silica
Matrix, 2006, Physics Department, National Taras Shevchenko Kyiv
University, Ukraine, pp. 1-25.*
(75) Inventor: Swadeshmukul Santra, Orlando, FL
H.W. Richardson, "Handbook of Copper Compounds and Applica
(US) tions" Copper Fungicides/batericides H.W. Richardson Editor, 1997,
Marcel Dekker, Inc.: New York, NY, pp. 93-122.
(73) Assignee: University of Central Florida Research Torgeson, D.C., ed. "Fungicides-An Advanced Treatise" Agricul
Foundation, Inc., Orlando, FL (US) tural and Industrial Applications and Enviromental Interaction, vol.
1, 1967, Academic Press: New York, NY, Ch. 6, p. 153-193 [chapter
( *) Notice: Subject to any disclaimer, the term ofthis Title: Formulation: Author: E. Somers.
Navarro, E., et al., In "Environmental behavior and ecotoxicity of
patent is extended or adjusted under 35
engineered nanoparticles to algae, plants, and fungi," Ecotoxicology,
U.S.C. 154(b) by 32 days. 2008, 17(5): pp. 372-386.
Oberdorster, G., et al., in Nanotoxicology: An Emerging Discipline
(21) Appl. No.: 13/527,691 Evolving from Studies ofUltrafine Particles, Environmental Health
Perspectives, 2005, 113(7): pp. 823-839.
(22) Filed: Jun.20,2012 S. Santra, et al., In "Fluorescence Lifetime Measurements to Deter
mine the Core-Shell Nanostructure of FITC-doped Silica
Nanoparticles: An Optical Approach to Evaluate Nanoparticle
Photo stability" Journal of Luminescence, 2006, 117(1) pp. 75-82.
Related U.S. Application Data Maniprasad, et al.; Novel Copper (Cu) Loaded Core-Shell Silica
Nanoparticles with Improved Cu Bioavailability: Synthesis, Charac
(62) Division of application No. 12/332,021, filed on Dec.
terization and Study of Antibacterial Properties; Journal of Biomedi
10, 2008, now Pat. No. 8,221,791. cal Nanotechnology; vol. 8, 1-9, 2012.
Maniprasad, et al.; Antimicrobial Properties of Copper and Silver
(51) Int. Cl. Loaded Silica Nanomaterials; Manuscript ID No. 1198620; to be
A61K9/14 (2006.01) submitted to the 36th International Conference on Advanced Ceram
AOJN59/20 (2006.01) ics and Composites (ICACC); Apr. 4, 2012.
(52) U.S. Cl. * cited by examiner
USPC ............ 424/484; 424/630; 514/499; 977/778
( 58) Field of Classification Search Primary Examiner - Sue Liu
USPC ................... 504/101; 424/484, 630; 514/499;
Assistant Examiner - Andriae M Holt
977/778
(74) Attorney, Agent, or Firm - Thomas Horstemeyer, LLP
See application file for complete search history.
(57) ABSTRACT
(56) References Cited
A silica-based nanoformulation and method is used to treat
U.S. PATENT DOCUMENTS citrus canker, inhibit the growth of mold and mildew, and add
nutrients to soil used for agricultural purposes. The nanotech
3,983,214 A 9/1976 Misato et al.
nology-enabled copper-loaded, silica nano formulation (CuS
3,992,146 A 1111976 Fazzalari
5,462,738 A 10/1995 LeFiles et al. iNP/NG) design is a "revolutionary re-invention" of Cu for
5,939,357 A 8/1999 Jones et al. safe application because it provides a formulation with maxi
6,471,976 Bl 10/2002 Taylor et al. mum abundance of ionic Cu, provides sustained and optimal
6,548,264 Bl 4/2003 Tan et al. Cu ion release for long-term disease protection, better adher
6,924,116 B2 8/2005 Tan et al.
ence to plant surfaces and structural surfaces due to gel-based
7,163,709 B2 1/2007 Cook et al.
7,226,610 B2 6/2007 Winniczuk nano structure of CuSiNG, thus avoiding multiple spray appli
7,332,351 B2 2/2008 Tan et al. cations and reducing the amount of Cu used in comparison to
2001/0051174 Al 12/2001 Staats existing Cu compounds without compromising antibacterial
2007 /0098806 Al 5/2007 Ismail et al.
activity. Thus, the silica-based nanoformulation releases cop
OTHER PUBLICATIONS per in non-toxic quantities to the environment and the silica
matrix provides an environmentally safe host material with a
Kikteva, T.A., Probing the Sol-Gel Conversion m the flexible design that is optimized to provide specific antifungal
Tetraethoxysilane/A lcohol/Water System with the Aid of Diffusion and antibacterial remediation using infrequent applications.
Controlled Fluorescence Quenching, 1997, Journal of Colloid and
Interface Science, vol. 193, pp. 163-166.* 6 Claims, 10 Drawing Sheets
U.S. Patent
Jan.21,2014 Sheet 1of10 US 8,632,811 Bl
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U.S. Patent
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U.S. Patent Jan.21,2014 Sheet 3of10 US 8,632,811 Bl
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U.S. Patent Jan.21,2014 Sheet 4of10 US 8,632,811 Bl
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Fig. 3
U.S. Patent Jan.21,2014 Sheet 5of10 US 8,632,811 Bl
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Cu loaded silica nanog.el
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U.S. Patent Jan.21,2014 Sheet 7of10 US 8,632,811 Bl
Fig. 6a Fig. 6b
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U.S. Patent Jan.21,2014 Sheet 8of10 US 8,632,811 Bl
Fig. 7a
Fig. 7b
50
Filter Paper Disc
Fig. 7c
No E.coli Growth
E.coli Colony