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About Practical Hand Outs-Molecular Biology (BIO302)
This document provides protocols for various molecular biology techniques, including: 1. DNA extraction from whole blood, involving cell lysis, protein separation, and DNA purification through steps like incubation with detergent and protease, salting out proteins, and alcohol precipitation. 2. Preparation of common reagents used in molecular biology like TE buffer, EDTA, NaCl solutions, ethidium bromide, and electrophoresis buffers. 3. Protocols for techniques like PCR, agarose gel electrophoresis, restriction digestion, transformation of bacteria, and real-time PCR. Safety considerations and use of controls are also discussed.
Detailed Information
| Author: | ['Anaya Malik'] |
|---|---|
| Publication Year: | 2019 |
| Pages: | 33 |
| Language: | English |
| Format: | |
| Price: | FREE |
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