Table Of ContentPHYTOCHEMISTRY
Volume 3
Marine Sources, Industrial Applications,
and Recent Advances
PHYTOCHEMISTRY
Volume 3
Marine Sources, Industrial Applications,
and Recent Advances
Edited by
Chukwuebuka Egbuna
Jonathan Chinenye Ifemeje, PhD
Shashank Kumar, PhD
Nadia Sharif, PhD
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Phytochemistry, Volume 3: Marine Sources, Industrial Applications, and Recent Advances
International Standard Book Number-13: 978-1-77188-761-8 (Hardcover)
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Phytochemistry, 3-volume set
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Library and Archives Canada Cataloguing in Publication
Phytochemistry / edited by Chukwuebuka Egbuna, Jonathan Chinenye Ifemeje, PhD,
Shashank Kumar, PhD, Nadia Sharif, PhD.
Includes bibliographical references and indexes.
Contents: Volume 3. Marine sources, industrial applications, and recent advances.
Issued in print and electronic formats.
ISBN 978-1-77188-761-8 (v. 3 : hardcover).--ISBN 978-1-77188-762-5 (set : hardcover).--
ISBN 978-0-429-42615-5 (v. 3 : PDF).--ISBN 978-0-429-42627-8 (set : PDF)
1. Botanical chemistry. I. Egbuna, Chukwuebuka, editor II. Ifemeje, Jonathan Chinenye, editor
III. Kumar, Shashank, editor IV. Sharif, Nadia, editor
QK861.P65 2019 572’.2 C2018-904824-7 C2018-904875-1
CIP data on file with US Library of C ongress
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ABOUT THE EDITORS
Chukwuebuka Egbuna
Chukwuebuka Egbuna is a chartered chemist, a chemical analyst, and an
academic researcher. He is a member of the Institute of Chartered Chemists
of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular
Biology (NSBMB), the Royal Society of Chemistry (RSC), United Kingdom,
and the Society of Quality Assurance (SQA), USA. He has been engaged in
a number of roles at New Divine Favor Pharmaceutical Industry Limited,
Akuzor Nkpor, Anambra State, Nigeria, and Chukwuemeka Odumegwu
Ojukwu University (COOU), Nigeria. He has attended series of conferences
and workshops and has collaboratively worked and published quite a number
of research articles in the domain of phytochemistry. He has edited books
with top publishers such as Springer Nature and Elsevier. He is a reviewer
and an editorial board member for various journals, including serving as a
website administrator for the Tropical Journal of Applied Natural Sciences
(TJANS), a journal of the faculty of Natural Sciences, COOU. His primary
research interests are in phytochemistry, food and medicinal chemistry,
analytical chemistry, and nutrition and toxicology. He obtained his BSc
and MSc degrees in biochemistry at Chukwuemeka Odumegwu Ojukwu
University.
Jonathan Chinenye Ifemeje, PhD
Jonathan Chinenye Ifemeje, PhD, is an Associate Professor in the Department
of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu
Ojukwu University, Nigeria. He obtained his PhD in applied biochemistry
from Nnamdi Azikiwe University, Awka, Nigeria, and his MSc degree in
nutrition and toxicology from the University of Port-Harcourt, Nigeria.
He has to his credits over 40 publications in both local and international
journals. Dr. Ifemeje is currently the Coordinator, Students Industrial
Work Experience Scheme (SIWES), COOU, and has served as an external
examiner for various institutions. He is the Managing Editor of the Tropical
Journal of Applied Natural Sciences and is serving as a reviewer and an
editorial board member for various journals. He has worked extensively in
the area of phytochemistry, nutrition, and toxicology. He is a member of
various institutes, including the Institute of Chartered Chemists of Nigeria
vi About the Editors
(ICCON), the Nigerian Society of Biochemistry and Molecular Biology
(NSBMB), and the Society of Quality Assurance (SQA).
Shashank Kumar, PhD
Shashank Kumar, PhD, is working as Assistant Professor at the Center for
Biochemistry and Microbial Sciences, Central University of Punjab, Bathinda,
India. He obtained his BSc, MSc, and PhD Biochemistry from the Department
of Biochemistry, University of Allahabad, India. He worked as Postdoctoral
Fellow at the Department of Biochemistry, King George’s Medical University,
Lucknow, India. Dr. Kumar has about 60 published scientific papers/reviews/
editorial articles/book chapters in various national and international peer-
reviewed journals and has been cited more than 1200 times. He has edited
several books on topics such as the “concepts in cell signaling,” “carbohydrate
metabolism: theory and practical approach,” and so forth. He has expertise
in the areas of free radical biology, cancer biology, characterization of plant
natural products, xenobiotic metabolism, and microbiology. He is familiar
with many biochemical techniques such as spectrophotometry, enzyme-linked
immunosorbent assay, electrophoresis, polymerase chain reaction, real-time
polymerase chain reaction, flow cytometry, thin-layer chromatography,
high-performance liquid chromatography, liquid chromatography–mass
spectrometry, cell culture, and microbiological techniques. He has presented
his research findings at more than 25 national/international conferences and
attended about 30 workshops at different universities and medical colleges
throughout the India. Dr. Kumar is a life time member of Italo-Latin American
Society of Ethnomedicine, and the Indian Sciences Congress Association, and
member of the Asian Council of Science Editors, Dubai, UAE, and Publication
Integrity and Ethics, London. He has been awarded the Junior/Senior and
Research Associate Fellowships formulated and funded by various Indian
agencies, such as Indian Council of Medical Research, University Grants
Commission, Council of Scientific and Industrial Research India. Dr. Kumar
laboratory has been funded by the University Grant Commission, India, and
the Department of Science and Technology, India, for working on effects of
various phytochemicals on cancer cell signaling pathway inhibition.
Nadia Sharif, PhD
Nadia Sharif, PhD, is currently working as a Visiting Lecturer at the Biotech-
nology Department of Lahore College for Women University, Lahore,
Pakistan. She obtained her MSc degree in Zoology from Bahauddin Zakariya
About the Editors vii
University, Multan, Pakistan, and did her PhD in Biotechnology, Department
of Lahore College for Women University, Lahore, Pakistan. She has been
awarded the Indigenous and International Fellowship (IRSIP) by the Higher
Education Commission, Pakistan. She has visited the Chemical and Biochem-
ical Engineering Department of The Johns Hopkins University, USA, for her
PhD research. She has about 20 published scientific papers/reviews/book
chapters in various national/international peer-reviewed journals. Dr Nadia
Sharif is serving as a reviewer to the British Microbiology Research Journal,
African Journal of Microbiology Research and Natural Product Journal. She
has expertise in areas of phycology, industrial biotechnology, microbiology,
molecular biology, biostatistics, and biochemistry. She is familiar with many
biochemical techniques such as spectrophotometry, electrophoresis, PCR,
ASE, TLC, HPLC, GC-MS, cell culture, and microbiological techniques. She
has a great interest in working for the sustainable use of natural resources.
She has presented her research findings in more than ten national conferences
and attended about 30 workshops at the majority of major universities and
colleges throughout Pakistan.
CONTENTS
Contributors ................................................................................................xiii
Abbreviations .............................................................................................xvii
Foreword .....................................................................................................xxi
Preface ......................................................................................................xxiii
PART I: Marine Sources of Secondary Metabolites ..............................1
1. Phytochemicals of Marine Origins ................................................................3
Nadia Sharif, Sana Nayab, Syeda Nazish Arshad, Neelma Munir, and Shagufta Naz
2. Marine Sponge Alkaloids: A Source of Novel Anticancer Agents .............35
Musarat Amina and Hanan M. Al-Yousef
3. Marine Antioxidants and Assay Methods ...................................................65
Onyeka Kingsley Nwosu and David Okechukwu Okeke
4. Extraction of Marine Phytochemicals: Methods and Techniques ............89
Nadia Sharif, Neelma Munir, and Shagufta Naz
PART II: Industrial and Medicinal Applications of
Phytochemicals ..............................................................................105
5. Biotechnology Approach to the Production of Phytochemicals:
An Introduction ...........................................................................................107
Hameed Shah, Andrew G. Mtewa, Chukwuebuka Egbuna, Anywar Godwin,
and Duncan C. Sesaazi
6. Secondary Metabolites Accumulation and Production Through
In Vitro Cultures ..........................................................................................131
Hussien M. Daffalla and Azza Migdam Elsheikh
7. Practical Processes Involved in the Production of Phytochemicals by
Plant Tissue Cultures ..................................................................................187
Hanan M. Al-Yousef
8. Medicinal and Industrial Applications of Bromelain ..............................205
Ramesh Kumar and Abhay K. Pandey
