Table Of ContentS
ID Drug Discovery Series/14
H
U
MEDICINAL CHEMISTRY •
G
E
Y
Phage Display in Biotechnology and Drug Discovery, Second ER
Edition provides a comprehensive view of the impact and
Phage
promise of phage display in drug discovery and biotechnology. P
h
Building on the success of its previous edition, the book dis-
a
cusses current theories, principles, and methods in the field g
e
and demonstrates applications for peptide phage display, Display in
D
protein phage display, and the development of novel antibodies.
i
s
p
Topics covered include:
l
a
Biotechnology
• Basic principles of phage display and methods y
developed for key applications in
• Applications for peptide phage display in the B
development of vaccines and high-throughput screens io and Drug
• Applications of phage display for protein characterization t
e
and drug discovery c
h
• Exploration of protein–protein interactions and enzyme n
o
substrate characteristics Discovery
l
o
• Engineering of protein stability, the identification and
g
mapping of protein–protein interactions, and the y
development of catalysts a
n
• Methods to construct and express libraries of peptides,
d
Second Edition
proteins, and antibodies
D
• Future challenges in the field r
u
g
The book provides readers with an overview of the amazing
D
breadth of the impact that phage display technology has had
i
s
on the study of proteins in general as well as the development
c
of proteins. It will be a valuable resource for those interested o EDITED BY
v
in using phage display and recombinant antibodies in basic e Sachdev S. Sidhu
r
research and drug discovery.
y
Clarence Ronald Geyer
SECOND
K11648 EDITION
ISBN: 978-1-4398-3649-1
90000
9 781439 836491
K11648_Cover.indd All Pages 1/26/15 11:45 AM
Phage
Display in
Biotechnology
and Drug
Discovery
Drug Discovery Series
Series Editor
Daniel Levy
1. Virtual Screening in Drug Discovery, edited by Juan Alvarez and
Brian Shoichet
2. Industrialization of Drug Discovery: From Target Selection Through Lead
Optimization, edited by Jeffrey S. Handen, Ph.D.
3. Phage Display in Biotechnology and Drug Discovery,
edited by Sachdev S. Sidhu
4. G Protein-Coupled Receptors in Drug Discovery, edited by Kenneth H.
Lundstrom and Mark L. Chiu
5. Handbook of Assay Development in Drug Discovery, edited by Lisa K. Minor
6. In Silico Technologies in Drug Target Identification and Validation, edited by
Darryl León and Scott Markel
7. Biochips as Pathways to Drug Discovery, edited by Andrew Carmen and
Gary Hardiman
8. Functional Protein Microarrays in Drug Discovery, edited by Paul F. Predki
9. Functional Informatics in Drug Discovery, edited by Sergey Ilyin
10. Methods in Microarray Normalization, edited by Phillip Stafford
11. Microarray Innovations: Technology and Experimentation,
edited by Gary Hardiman
12. Protein Discovery Technologies, edited by Renata Pasqualini and
Wadih Arap
13. Pharmacoeconomics: From Theory to Practice,
edited by Renée J. G. Arnold
14. Phage Display in Biotechnology and Drug Discovery, Second Edition,
edited by Sachdev S. Sidhu, Clarence Ronald Geyer
Phage
Display in
Biotechnology
and Drug
Discovery
Second Edition
EDITED BY
Sachdev S. Sidhu
Clarence Ronald Geyer
CRC Press
Taylor & Francis Group
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Boca Raton, FL 33487-2742
© 2015 by Taylor & Francis Group, LLC
CRC Press is an imprint of Taylor & Francis Group, an Informa business
No claim to original U.S. Government works
Version Date: 20150128
International Standard Book Number-13: 978-1-4398-3650-7 (eBook - PDF)
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Contents
Foreword ...................................................................................................................ix
Preface.......................................................................................................................xi
Contributors ...........................................................................................................xiii
Chapter 1
Filamentous Bacteriophage Structure and Biology ...................................................1
Diane J. Rodi, Suneeta Mandava, and Lee Makowski
Chapter 2
Vectors and Modes of Display .................................................................................43
Valery A. Petrenko and George P. Smith
Chapter 3
Methods for the Construction of Phage-Displayed Libraries ..................................75
Frederic A. Fellouse and Gabor Pal
Chapter 4
Selection and Screening Strategies ..........................................................................97
Mark S. Dennis
Chapter 5
Leveraging Synthetic Phage-Antibody Libraries for Panning on the
Mammalian Cell Surface .......................................................................................113
Jelena Tomic, Megan McLaughlin, Traver Hart, Sachdev S. Sidhu,
and Jason Moffat
Chapter 6
Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines ....123
Nienke E. van Houten and Jamie K. Scott
Chapter 7
Exploring Protein–Protein Interactions Using Peptide Libraries Displayed
on Phage .................................................................................................................181
Kurt Deshayes and Jacob Corn
v
vi Contents
Chapter 8
Substrate Phage Display .........................................................................................205
Shuichi Ohkubo
Chapter 9
Mapping Intracellular Protein Networks ...............................................................229
Malgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. Kay
Chapter 10
High-Throughput and High-Content Screening Using Peptides ...........................249
Robert O. Carlson, Robin Hyde-DeRuyscher, and Paul T. Hamilton
Chapter 11
Engineering Protein Folding and Stability ............................................................275
Mihriban Tuna and Derek N. Woolfson
Chapter 12
Identification of Natural Protein–Protein Interactions with cDNA Libraries .......295
Reto Crameri, Claudio Rhyner, Michael Weichel, Sabine Flückiger,
and Zoltan Konthur
Chapter 13
Mapping Protein Functional Epitopes ...................................................................311
Sara K. Avrantinis and Gregory A. Weiss
Chapter 14
Selections for Enzymatic Catalysts ........................................................................325
Julian Bertschinger, Christian Heinis, and Dario Neri
Chapter 15
Antibody Humanization and Affinity Maturation Using Phage Display ..............347
Jonathan S. Marvin and Henry B. Lowman
Chapter 16
Antibody Libraries from Immunized Repertoires .................................................373
Jody D. Berry and Mikhail Popkov
Contents vii
Chapter 17
Naive Antibody Libraries from Natural Repertoires .............................................455
Claire L. Dobson, Ralph R. Minter, and Celia P. Hart-Shorrock
Chapter 18
Synthetic Antibody Libraries .................................................................................495
Frederic A. Fellouse and Sachdev S. Sidhu
Chapter 19
Engineering Antibody Fragments for Intracellular Applications ..........................521
Jianghai Liu and Clarence Ronald Geyer
