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About Of Brachypodium Distachyon: Agrobacterium-Mediated Transformation
This document describes a highly efficient Agrobacterium-mediated transformation protocol for Brachypodium distachyon. The protocol uses callus induced from immature embryos and takes 20-30 weeks from dissecting the embryos to harvesting transgenic T1 seeds. Transformation efficiencies are typically 40-70% when using hygromycin or paromomycin selection. The protocol has been optimized for B. distachyon lines Bd21 and Bd21-3 and has been used to create over 22,000 T-DNA insertional mutants.
Detailed Information
| Author: | ['Luis Gómez'] |
|---|---|
| Publication Year: | 2019 |
| Pages: | 16 |
| Language: | English |
| Format: | |
| Price: | FREE |
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