Metabolism of Amino Acids and Amines Part A

723 Pages·1970·36.78 MB·English

by  Herbert Tabor

Contributors to Volume XVIIA
Article numbers are shown in parentheses following the names of contributors.
Affiliations listed are current.
ORUBAZOK ADACHI (81), Department of HPESOJ M. OVLAC (107), Department of Bio-
,yrtszmehcoiB Osaka University Dental School, chemistry, Cornell University, Ithaca, New
K ita-ku, Osaka,Japan York
IJNEK 1KA (111, 112, 113), lnstituteJor En- YOON SANG CHo-CHUNG (105), Department
zyme Research, School of Medicine, Toku- oJ Biological Sciences, Purdue University,
shima University, Tokushima, Japan West LaJayette, Indiana
LUAP M. NOSREDNA (22), Chemistry Depart- SEGROEG N. COHEN (91a, 91b, 93), Service
ment, Southern Illinois University, Carbon- de Physiologie Microbienne, lnstitut Pasteur,
dale, Illinois Paris, France
TRAUTS M. ARFIN (100), School of Medicine, PHILIP P. COHEN (21, 117), Department o
Department of Biochemistry, University of Physiological Chemistry, The University o
Pittsburgh, Pittsburgh, Pa. Wisconsin, Madison, Wisconsin
F. B. GNORTSMRA (102), Department of Bio- D. L. NAMELOC (17), The Jackson Laboratory,
chemistry, North Carolina State University, Bar Harbor, Maine
Raleigh, North Carolina OCSECNARF INOCNOC (145), Istituto di Chi-
TREBOR L. GNORTSMRA (2), Department oJ mica Biologica, University of Ferrara, Fer-
Biological Chemistry, University of Michigan, rara, Italy
Ann Arbor, Michigan DLAREJ L. VLLENNOC (114), Department o
NODROG B. YELIAB (116), RockeJeller Foun- Biochemistry, University of North Dakota,
dation, Bangkok, Thailand School of Medicine, Grand Forks, North
DORIS YKSNILAB (43c), The South dJrican Dakota
Institute Jor Medical Research, Johannes- RONIM J. COON (115), Department '?( Bio-
burg, South AJrica logical Chemistry, University of Michigan,
GuY T. YRRAB (10), Deputy Director, Division Ann Arbor, Michigan
o Technical Service, Bureau "?~ ,smot~.uC A. J. L. COOPER (addendum to 136), De-
Washington, D.C. partment 'Jo Biochemistry, Cornell Univer-
A. M. LEO C. ELLTAB (18, 19), Catedra de sity Medical College, New York, New York
Quimica Biologica, Facultad de Ciencias DRANOEL NAMROC ( 1 18), Assistant Proessor
Exactas y Naturales, Universidad ed Buenos o Biochemistry, TuJ~s University, School oJ
Aires, Buenos Aires, Argentina Medicine and Dental Medicine, Boston,
ARDNAS NEHTELB (39), Department oJChem- Massachusetts
istry, San Francisco State College, San R. G. H. NOTTOC (71), Royal Children~ Hos-
Francisco, CaliJbrnia pital Research Foundation, Parkville, Vic-
JOE A. NEDWOB (114), Department o Bio- toria, Australia
chemistry, University of Florida, College SAMOHT E. NOTHGIERC (46), MRC Labora-
of Medicine, Gainesville, Florida tory 0/' Molecular Biology, Cambridge, En-
NAHTAN BROT (82), Roche Institute of Mo- gland
lecular Biology, Nutley, New Jersey JUNE L. DAHL (139), Department oJ'Pharma-
ECURB MAHNRUB (14), Department of Chem- cology, University of Wisconsin, Medical
istry, Utah State University, Logan, Utah Center, Madison, Wisconsin
R. O. BURNS (108), Department of Micro- NAED J. RENNAD (85b, 114), St. Jude Chil-
ygoloib & Immunology, Duke University dren's Research Hospital, Memphis, Ten-
Medical Center, Durham, North Carolina
nessee
vi CONTRIBUTORS TO VOLUME XVIIA
ATNASARP DATTA (92), Department of Bio- A. M. ALOMARREF (18, 19), Catedra de Qai-
logical Chemistry, University of Michigan, mica Biologica, Facultad de Ciencias Exactas
Ann Arbor, Michigan y Naturales, Universidad de Buenos Aires,
DNALWOR H. DAVIS (4), Department "o Buenos Aires, Argentina
Botany, University °f Michigan, Ann Arbor, DLAREG R. FINK (3), Dept. of Genetics, Plant
Michigan Science Bldg., Cornell University, Ithaca, N.Y.
DIVAD M. NOSWAD (143), Department o ETIREUGRAM FLING (77), Department oJ
Neurology, Harvard Medical ,loohcS Boston, Biology, Cal!/brnia Institute of ,ygolonhceT
sttesuhcassaM Pasadena, California
JEAN NAVAD (70a, 70b), Columbia University J. E. FOLK (27), Laboratory o/ ,yrtsimehcoiB
College of Physicians and Surgeons, Depart- National Institute oJ Dental Research, Na-
ment of ,yrtsimehcoiB New York, New York tional Institutes of Health, Bethesda, Mary-
JOHN A. DEMOSS (48), Department ,ygoloiBJo land
University of ,ainrbJilaC La Jolla, ainrbJilaC SELRAHC A. FREE (10), Department of Bio-
RETLAW B. DEMPSEV (116), Biochemistry chemical Pharmacology, Squibb Institute Jbr
Research, VA Hospital, Dallas, Texas Medical Research, New Brunswick, New
G~ZA SEN~D (5, 28, 29, 30), Institute of Medi- Jersey
cal Chemistry, University Medical School, TSNRE ESEERF (11), Laboratory of Molecular
Budapest, Hungary Biology, National Institute of Neurological
NALA W. DENNIS (43C), Department of Bio- Diseases and Blindness, National Institutes
chemistry, University of Toronto, Toronto, of Health, Bethesda, Maryland
Canada MLEHLIW R. LLESIRF (141), Department o/
MLOCLAM DIXON (94), Department of Bio- ,yrtsimehcoiB New Jersey College of Medicine
chemistry, University 0 Cambridge, Cam- and Dentistry, Newark, New Jersey
,egdirb England M. AKOIJUF (73), Department oJBiochemistry,
AMLED DOHERTY (119), Biochemistry De- Medical School, Osaka University, Osaka,
partment, University College, London, Gower Japan
St., London, W.C.I, England HITOSHI AWASIJUF (66), Department of Medi-
J. G. EBBON (20), Department o Chemical cal Chemistry, Kyoto University, Faculty of
,ygolohtaP St. Marys Hospital Medical ,loohcS Medicine, Sakyo-ku, Kyoto,J apan
St. Marys Hospital, London, England KNARF RENTREAG (48), Graduate School oJ
DRAHRrBE EBNER (131), Biochemisches ln- Biomedical Sciences, Biology Division, Oak
stitut, D-78 Freiburg im Breisgau, West Ridge National Laboratory (ORNL), Oak
Germany Ridge, Tenn.
A. F. EGAN (47), Department of yrtsimehcoiB SOLRAC ODECNAG (131), Centro de Investiga-
and Molecular Biology, Biological Labora- ciones Biologicas, Instituto de Enzimologia,
,seirot Harvard University, Cambridge, Mas- Madrid, Spain
sttesuhcas JOHN NESUAHSREMREG (49), Department oJ
W. H. TTOILLE (15), rosseJorP of ,yrtsimehcoiB Biology and McCollum Pratt Institute, The
Department of Biochemistry, The University Johns Hopkins University, Baltimore, Mary-
of Adelaide, Adelaide, South Australia land
SNAH E. REGREBNEPPE (143), lnstitut de Bio- DRAWOH GEST (92), Department of Micro-
chimie de L'Universit~, Neuchhtel, Switzer- ,ygoloib Indiana University, Bloomington,
land Indiana
DRANOEL A. FAHIEN (ll7), Department of R. G. GIBBs (142), Department of Agricultural
Pharmacology, University of Wisconsin, and Forest Zoology, University College of N.
Madison, Wisconsin Wales, Bangor, N. Wales
ESIOCNARF YLLEK-ZOCLAF (91b), Service de KNARF GIBSON (45, 47, 71), Department of
Physiologie Microbienne, lnstitut Pasteur, ,yrtsimehcoiB John Curtin School of Medical
Paris, France Research, Canberra City, A. .C T., Australia
CONTRIBUTORS TO VOLUME XVIIA vii
NNA GINSBURG (132), National Institutes 'Jo EGROEG HORN (77), Department of ,ygoloiB
Health, Department ?* Health, Education, Calibrnia Institute of Technology, Pasa-
and We(are, Public Health Service, Bethesda, dena, ainrbJilaC
Maryland DRANREB ZTIWOROH (97), Department of Bio-
LuIS RESALG (124), Department 'o Biological ,yrtsimehc Cornell University Medical Col-
Chemistry, Washington University School of ,egel New York, New York
Medicine, St. Louis, Missouri NAMRON H. ZTIWOROH (77), Department ff~
E. BULLOG (42), Columbia University, Depart- Biology, CaliJbrnia Institute of ,ygolonhceT
ment of Biochemistry, New York, New York Pasadena, ainroJilaC
D. K. RENNARG (80), Department oJMedicine, ARIKA ARAHIHCI (11 , 112, 113), Institute
University of Iowa College of Medicine, Iowa .or Enzyme Research, School ~ Medicine,
,ytiC Iowa Tokushima University, Tokushima, Japan
OCIRNE GRAZI (145), lstituto di Chimica ATARA ICnIYAMA (56, 58), Department of
Biologica, University of Ferrara, Ferrara, Medical Chemistry, Kyoto University, Faculty
Italy of Medicine, Sakyo-ku, ,otoyK Japan
M. NIETSNIRG (18, 19), Catedra de Quimica DNIVRA RADMANI (118), Instructor in Medi-
Biologica, Facultad de Ciencias Exactas y cine (Biochemistry), Tufts University School
Naturales, Universidad de Buenos Aires, of Medicine, Circulation Laboratory, Boston
Buenos Aires, Argentina City Hospital, Boston, sttw~uhcassaM
NOSMAS R. SSORG (104, 106, 107), Depart- E. A. IRVING (15), Institute of Medical and
ment oJ ,yrtsimehcoiB Duke University Medi- Veterinary Science, Adelaide, South Australia
cal Center, Durham, North Carolina URUZUY ARUMIHSI (52), Department o/Medi-
NODROG FFORUG (74), Section no Intermediary cal Chemistry, Kyoto University, Faculty of
Metabolism, Laboratory of Biomedical Sci- Medicine, Sakyo-ku, ,otoyK Japan
ences, National Institute of Child Health OUZAK IKAZI (12), Department of Agricultural
and Human Development, Bethesda, Mary- ,yrtsimehC Faculty "Jo Agriculture, Tohoku
land ,ytisrevinU Sendal,Japan
LLEWOL P. HAgER (84, 120), Biochemistry MAILLIW B. YBOKAJ (60), Section on Enzymes
Department, University of Illinois, Urbana, and National Institute oJ Arthritis and
Illinois Metabolic Diseases, N.I.H., Bethesda, Mary-
HTENNEK R. NOSNAH (72a), The Connecticut land
Agricultural Experiment Station, New Haven, W. YRRET SNIKNEJ (7, 9, 31), Department oJ
tucitcennoC ,yrtsimehC Indiana University, Bloomington,
NIGEL DROFRAH (2), Department of ,ygoloiB Indiana
Princeton University, Princeton, New Jersey NAVE E. JONES (26), Laboratory of Nutritional
HSIDNATS C. NAMTRAH (134), Department ,yrtsimehcoiB Department of Animal Science,
of Chemistry, Boston University, Boston, North Carolina State University, Raleigh,
sttesuhcassaM North Carolina
NYLEVE A. HAVre (72a), The Connecticut YRAM NELLE JONES (32), Department of Bio-
Agricultural Experiment Station, New Haven, ,yrtsimehc The University of North Carolina,
Connecticut Chapel Hill, North Carolina
UMASO IHSIAYAH (53, 58, 61), Department CRAM JULIUS (10), Carter-Wallace Inc., Cran-
of Medical Chemistry, Kyoto University, ,yrub New Jersey
Faculty of Medicine, Sakyo-ku, Kyoto, Japan RHIDA BASSAK (144), Department of Bio-
EOROEG D. NAMEGEH (88, 89, 93), Depart- ,yrtsimehc College de France, Paris, France
ment of Bacteriology and Immunology, Uni- DRAWDE KATZ (68), Department 'Jo Micro-
versity of California, Berkeley, Calijbrnia ,ygoloib Georgetown University School of
TUMLEH REZLOH ( 131 ), Biochemisches Insti- Medicine, Washington, D.C.
tut, Universitat Freiburg im Breisgau, 78 RUOMYZS NAMFUAK (75, 76), Laboratory oJ
Freiburg im Breisgau, West Germany ,yrtsimehcorueN National Institutes of Health,
VIII ,°° CONTRIBUTORS TO VOLUME XVIIA
National Institute of Mental Health, Be- M. NOIRRAC-ZENITRAM (9), Department of
,adseht Maryland ,yrtsimehC University of Notre Dame, Notre
REGOR H. TTENNEK (2), Department oBiol- ,emaD Indiana
ogy, University of ,ainrbJilaC San ,ogeiD DRAWOH S. NOSAM (79), Department of Bio-
La Jolla, ainroilaC ,yrtsimehc University of Oregon, School of
K. KIRITANI (99, 101), Department of Bac- ,enicideM ,dnaltroP nogerO
,ygoloiret Medical School, Kanazawa Uni- OERAT AWAZUSTAM (6), Department of En-
,ytisrev ,awazanaK Japan emyz ,yrtsimehC Institute of Enzyme ,hcraeseR
HIROSm KITA (83), The Institute of dooF loohcS of Medicine, Tokushima ,ytisrevinU
,yrtsimehC Kitaku, Osaka,Japan ,amihsukoT Japan
S. M. KLEIN (140), Department "fo Micro- NOTLA MEIs'rER (22, 90a, 97, 121, 122, 126,
,ygoloib Brigham Young University, Provo, 129, 135. Addendum to 136.) tnemtrapeD
hatU of ,yrtsimehcoiB UenroC University Medical
RETEP F. SELWONK (43a, 44), Astbury .tpeD ,egelloC New ,kroY N. Y.
of ,scisyhpoiB University of Leeds, England TREBOR G. REYEM (49), Department oJ Bio-
W. ENEGUE XONK (50, 136), Cancer hcraeseR ,yrtsimehc University of Washington, loohcS
Institute, New England ssenocaeD ,latipsoH of ,enicideM ,elttaeS notgnihsaW
,notsoB sttesuhcassaM EDITH WILSON MILES (90a, 90b), National
SHUHE! mSAVABOK (61), Institute Jbr Virus Institutes of Health, Department of Health,
,hcraeseR Kyoto University, ,otoyK Japan Education and Welfare, Public Health Ser-
RETNVG B. WAHLHOK (103), Department of ,eciv ,adsehteB Maryland
,yrtsimehcoiB Purdue University, ,etteyaJaL R. C. NACILLIM (43a),* National Institutes of
Indiana Health, ,adsehteB Maryland
TstmE KOSUGE (55), Department of Plant KlvlZ EVADLOM (135), Department of Bio-
,ygolohtaP egelloC of ,erutlucirgA ytisrevinU ,yrtsimehc University of ,hgrubsttiP loohcS of
of ,ainrofilaC Davis, ainrbJilaC ,enicideM ,hgrubsttiP Pennsylvania
R. PREM KUMAR (62), Department of Bio- K. MOORE (63), Department of ,ygoloiB
,yrtsimehc Indian Institute of ,ecneicS Ban- s'pohsiB ,ytisrevinU ,ellivxonniL P.Q., adanaC
,erolag India TREBOR NAGROM (93), Department of Bac-
TREBREH Z. KIHCPUK (136), Mallory Institute ,ygoloiret University of ,ainrbJilaC ,yelekreB
ro' ,ygolohtaP Boston City Hospital, ,notsoB ainrbJilaC
sttesuhcassaM Y. MORINO (54, 73), Department of -mehcoiB
T. R. YRAEL (103), Department of -mehcoiB istry, Osaka University Medical ,loohcS
istry, Purdue University, West ,etteyafaL K itaku, napaJ,akasO
Indiana J. G. MORRIS (142), Department of -mehcoiB
ECURB GREBNEVEL (23, 37a, 125), Depart- ,yrtsi University of ,retsecieL ,retsecieL En-
ment of lacigoloiB ,yrtsimehC University of .dnalg
Michigan, School of Medicine, Ann ,robrA NITRAM NOSIRROM (85a, 85b), St.Jude -lihC
Michigan dren's Research Hospital, Memphis, Ten-
J. G. LEWN (44), National Institutes of ,htlaeH eessen
.adsehteB Maryland MivoKo ARUMAKAN (32), lacimehcoiB Insti-
OMSOC G. EIZNEKCAM (141), Department of tute, Kyoto Prefectural University, ,otoyK
,yrtsimehcoiB University of odaroloC Medical Japan
,loohcS Denver, odaroloC UBONEGIHS ARUMAKAN (56, 59), tnemtrapeD
WILHAM L. NALLELGM (24b, 25, 27), -eD of ,scirtaireG Kyoto University Faculty of
partment of ,ygolohtaP egelloC of snaicisyhP Medicine, Yoshida, Sakyo-ku, Kyoto, Japan
and Surgeons, Columbia University, New URONIM ONAKAN (86), Department of Bio-
,kroY New kroY ,yrtsimehc School of Medicine, Gunma Uni-
TERAGRAM LLAHSRAM (21), Department of ,ytisrev ihsabeaM Gunma,Japan
lacigoloisyhP ,yrtsimehC University of Wis-
,nisnoc Madison, nisnocsiW *deceased.
CONTRIBUTORS TO VOLUME XVIIA ix
ATSUSHI AWAZAKAN (64), Department of Molecular Biology, Vanderbilt University,
Biology, The Johns Hopkins University, Nashville, Tennessee
Baltimore, Maryland RUOMYES H. ZTNAREMOP (78), Department
TERUKO AWAZARAN (64), Department oj' oJ Biochemistry, University Jo Maryland,
Medical Chemistry, Faculty of Medicine, School of Medicine, Baltimore, Maryland
Kyoto University, Kyoto, Japan ENNA-ESIUOL LEDARP (144), Department oJ
ENNAEJ A. NOSLEN (97), Department of Physi- Biochemistry, College de France, Paris, France
ological Chemistry, Johns Hopkins University P. V. ABBUS OAR (62, 63, 69, 72b), Depart-
School of Medicine, Baltimore, Maryland ment of Biochemistry, Indian Institute of
RITA M. NOSLEN (79), Department of Bio- Science, Bangalore-I 2, India
chemistry, University of Oregon Medical HARAS RENTAR (34, 35), Department of Bio-
School, Portland, Oregon chemistry, The Public Health Research Insti-
A. REGREBUEN (I 3, 20), Department of Chem- tute of the City of New York, New York, New
ical Pathology, St. Marys Hospital Medical York
School, St. Mary's Hospital, London, England ENNAOJ M. LEVAR (96), Department of Chem-
IMOTUSAY AKUZIHSIN (58, 59), Department istry, The University of Texas, Austin, Texas
of Biochemistry, Kobe University School "Jo AEL VEtrSER (50), Hebrew University, Hadas-
Medicine, I kutaku, K ,ebo Japan sah Medical School, Jerasalem, Israel
GNAEURNUB NROPMOYIN (139), Department K. E. NOSDRAHCIR (8), Department oPhysio-
of Biochemistry, Siriraj Hospital Medical logical Chemistry, Tile Ohio State University,
School, Bangkok, Thailand Columbus, Ohio
MAHARBA YKSDORGOVON (90a), Weiszman TREBOR A. OXZNOR (129), Department of Bio-
Institute, Rehoboth, Israel chemistry, Michigan State University, Lan-
MITSUHmO NOZAm (65), Department of sing, Michigan
Medical Chemistry, Kyoto University, Faculty TREBOR J. RooN (37a), Department of Bio-
of Medicine, Sakyo-ku, K ,otoy Japan chemistry, University of Cal!brnia, Berkeley,
Koicm OGATA (41), Department of Agricul- CaliJbrnia
tural Chemistry, Kyoto University, Sakyo, DLAREG A. LAHTNESOR (22), Department of
K yoto, J apan Biology, Case Western Reserve University,
IHSORIH OTOMAKO (57), Department of Medi- Cleveland, Ohio
cal Chemistry, Kyoto University, Faculty Jo J. R. ROTrt (1), Department of Molecular
Medicine, Sakyo-ku, K ,otoy Japan Biology, University of California, Berkeley,
CaliJbrnia
ANNA IKCUMOLO (40), Department of Bio-
chemistry, College de France, Paris, France W. BRUCE ROWE (129), Department of Bio-
chemistry, Cornell University Medical Center,
MAma OaLowsm (122, 126), Department
New York, New York
o Biochemistry, Cornell University Medical
College, New York, New York DRAHCIR D. SREGAS (140), Department of
Microbiology, Brigham Young University,
L. N. ORNSTON (67), Biology Department,
Provo, Utah
Kline Biology Tower, Yale University, New
NOTLIM SAIER, JR. (7), Department of Bio-
Haven, Conn.
chemistry, University of CaliJornia, Berkeley,
LUAP M. NAMKCAP (60), Department of Psy- CaliJbrnia
chiatry, Washington University School of
H. A. HeXVOCNAS (19), Catedra de Quimica
Medicine, St. Louis, Missouri
Biologica, Facultad de Ciencias Exactas y
HARAS J. SNOSRAP (108), Department of Naturales, Universidad de Buenos Aires,
Microbiology, Duke University Medical Cen- Buenos Aires, Argentina
ter, Durham, North Carolina
B. D. LAWNAS (109), University of Toronto,
JEAN PEH-CHEN LI (78), Department of Bio- Department of Cell Biology, Medical Sciences
chemistry, University ?~ Maryland, School o Building, Toronto, Ontario
Medicine, Baltimore, Maryland
TREBOR T. EKMIHCS (33, 36, 37, 38, 51),
EIRAM L. ST. PIERRE (2), Department Jo Department of Pharmacology, StanJord Uni-
X CONTRIBUTORS TO VOLUME XVIIA
versity, School of Medicine, Palo Alto, Cali- Institute of Biochemistry, Munich, West
fornia Germany
DLORAH L. LAGES (6),Department of ,ygoloiB LEWIS D. STEGINK (115), Department of
State University of New York, Buffalo, New Pediatrics, College of Medicine, University
York of lowa, Iowa City, Iowa
Stud SENOH (83), The Central Research In- KCAJ L. REGNIMORTS (12, 95, 139), Harvard
stitute, Suntory Limited, Kitaku, Osaka, University, Biological Laboratories, Cam-
Japan ,egdirb sttesuhcassaM
KCIREDERF j. ED SERRES (4), Biology Division, P. V. ABBUS OAR (62, 63, 69, 72b), Depart-
Oak Ridge National Laboratory, Oak Ridge, ment of Biochemistry, Indian Institute of
Tennessee ,ecneicS Bangalore, India
TTENNEB M. ORIPAHS ( 130, 133), yrotarobaL UROBON AKOEUS (2), Departments of Bio-
of ,yrtsimehcoiB National Institutes of Health, yrtsimehc and Biology, Princeton University,
Bethesda, Maryland Princeton, New Jersey
W. V. SHAW (123), Department oflnfectious DNUMGIS R. DNXKSUS (49), McCollum Pratt
,sesaesiD Veterans Administration Hospital, Institute, The Johns Hopkins University,
New York, New York Baltimore, Maryland
DIVAD SHEMIN (16), Biochemistry Division, AILEC WHITE TABOR (137), National Insti-
Department of Chemistry, Northwestern Uni- tutes of Health, Bethesda, Maryland
,ytisrev Evanston, Illinois G. H. TAIT (20), Department of Chemical
Ross NAMIHS (76), Department of Biological ,ygolohtaP St. M arys Hospital Medical ,loohcS
,yrtsimehC The M. S. Hershey Medical Cen- St. M syra Hospital, London, England
ter, Pennsylvania State University, Hershey, ORIHSOY ADEKAT (81), Department of -DiB
Pennsylvania ,yrtsimehc Osaka University Dental School,
E. SNELL (54), Department of ,yrtsimehcoiB Kitaku, Osaka,Japan
University of California, Berkeley, California HASSADAH TAMIR (48a), Department of -DiB
IJNEK ADOS (41, 90a), Institute for Chemical ,yrtsimehc College of Physicians and Sur-
Research, Kyoto University, Uji, Kyoto-fu, geons, Columbia University, New York, N.Y.
Japan HSERUS S. TATE (90a), Department of -DiB
YASDNIL GALE WORRAPS (90b), C.SJ.R.O., ,yrtsimehc Cornell University Medical Col-
Division of Protein Chemistry, Parkville, ,egel New York, New York
,airotciV Australia TREBOR T. ROLYAT (110), BiD-Medical Divi-
DIVAD B. NOSNIRPS (42, 43a, 44, 70a, 70b), sion, Lawrence Radiation Laboratory, Liver-
Columbia University, College of Physicians ,erom California
and Surgeons, Dept. of Biochemistry, New JOHN F. NOSPMOHT (128), .U S. Department
York of Agriculture, Agricultural Research Ser-
N. .S SREELEE~ (62), Department of -mehcoiB vice, .U S. Plant, Soil and Nutrition Labora-
,yrtsi Indian Institute of Science, Bangalore, ,yrot Ithaca, New York
India J. S. NOSPMOHT (8), Instrument Products Divi-
P. R. NASAVINIRS (48a), Dept. of Biochem- sion, E. I. Du Pont De Nemours & Company,
istry, College of Physicians & Surgeons, Wilmington, Delaware
Columbia University, New York, N.Y. G. M. SNIKMOT (80), Department of Biochem-
E. R. NAMTDATS (123, 130), Laboratory of ,yrtsi University of California Medical Cen-
,yrtsimehcoiB National Heart and Lung In- ,ret San ,ocsicnarF California
,etutits National Institutes of Health, ,adsehteB G. H. N. SREWOT (63, 72b), Department of
Maryland Botany, University of British Columbia,
ASSERHT C, NAMTDATS (138), Laboratory of Vancouver, Canada
,yrtsimehcoiB National Heart and Lung In- OLOAP IHCAB-AFFURT (91a), Service de Phys-
stitute, Bethesda, Maryland iolige Microbienne, lnstitut Pasteur, Paris,
RETLAW L. REUABNEDUATS (95), Max Planck France
CONTRIBUTORS TO VOLUME XVIIA xi
HSIN TSAI (31), Department of Molecular ARIAV P. RENLLEW (22, 129), Department of
,ygoloiB Max Planck Institute for Experi- ,yrtsimehcoiB Cornell University Medical -loC
latnem Medicine, ,negnittbG ynamreG ,egel New York, New York
JOHN M. TURNER (13), yrtsimehcoiB Depart- JOHN C. WRISTON, JR. (98), Department of
ment, University of Liverpool, ,loopreviL ,yrtsimehC University of ,erawaleD Newark,
England erawaleD
H. E. REGRABMU (105), Department of Bio- Saozo OTOMAMAY (53), Department of Medi-
lacigol Sciences, Purdue University, Lafay- lac Chemistry, Kyoto University Faculty of
,ette Indiana ,enicideM ,uk-oykaS ,otoyK Japan
C. S. NAHTANAYDIAV (62, 69), Department SELRAHC YKSFONAY (46), Department of Bio-
of ,yrtsimehcoiB Indian Institute of ,ecneicS lacigol Sciences, Stanford University, Stan-
,erolagnaB India ford, ainrofilaC
NEYUGN NAV THOAI (40, 144), Department of SENGA YP (50), Rutgers Medical ,loohcS Rut-
,yrtsimehcoiB egelloC ed France, Paris, ecnarF gers The State University, Department of
YRNEH J. LEGOV (24a, 24b, 25, 26, 27), -eD ,yrtsimehcoiB New Brunswick, New Jersey
partment of ,ygolohtaP egelloC of snaicisyhP USTXMAKAT YORIFUJI (41), Department of
and Surgeons of aibmuloC University, New Agricultural ,yrtsimehC Shinshu ,ytisrevinU
,kroY N. .Y Kamiina-Gun, Nagano-Ken,Japan
Hmosm WAnA (73), Department of Bio- ARIKA ADXttSOY (11), Department of ,enicideM
,yrtsimehc Osaka University School of Medi- noisiviD of Medical ,sciteneG loohcS of Medi-
,enic Osaka,Japan cine, University of Washington, Seattle,
R. P. RENGAW (99, 101, 102), Department of notgnihsaW
,ygolooZ The University of Texas at Austin, H. NIKLAZ (42), Department of ,yrtsimehcoiB
Austin, Texas Purdue ,ytisrevinU ,etteyafaL Indiana
SEMAJ B. REKLAW (146), Department of Biol- TNECNXV G. INONNAZ (87), Department of
,ygo W. M. Rice University, Houston, Texas ,ygolocamrahP New York University Medical
TERAGRAM S. REKLAW (146), Department of ,retneC New York, New York
,ygoloiB Rice University, Houston, Texas M. W. ZINK (109), Department of ,ygoloiB
TREBREH HCABSSXEW (68, 82), Roche Insti- ytisrevinU of ,nawehctaksaS ,nootaksaS Can-
tute "o Molecular ,ygoloiB Nutley, New yesreJ ada
Preface
Volume XVII is designed to provide a comprehensive coverage of the
recent enzymological developments in the field of amino acids and
amines. With the extensive development of enzymological techniques,
the literature concerned with this area has increased rapidly. Due to
the large amount of material, it has been necessary to divide Volume
XVII into two parts.
The first section of Volume XVIIA includes an extensive description
of various microbiological and genetic techniques that have become
major tools in the study of amino acid metabolism in recent years. Since
there has been a paucity of manuals giving specific details of these
methods, we hope that the articles published here will be of considerable
value. The remainder of the material is arranged in sections which are
in a roughly alphabetical order: alanine to guanido compounds in
XVIIA and the remaining amino acids and amine metabolism sections
in XVIIB. The alphabetical arrangement is necessarily arbitrary as
there is considerable crossover among the sections, and, in some cases,
several amino acids (such as valine, leucine, and isoleucine) are covered
together under a single heading (such as branched chain amino acids).
We hope that the cross-references at the beginning of each section and
in the index will alleviate some of the resultant problems.
Recent methodological developments have involved automated
techniques for amino acid analyses. For the most part, these procedures
have not been described in this volume since these techniques, as well
as a number of other related analytical procedures, have been covered
in Volume XI ("Enzyme Structure," C. H. Werner Hirs, ed.).
We wish to thank all of the contributors for their articles and for
their many helpful suggestions.
xiii
METHODS IN ENZYMOLOGY
DETIDE YB
Sidney P. Colowiek and Nathan O. I~plan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xxix
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan O. Kaplan
VOLUME VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD AND VICTOR GINSBURG
VOLUME IX. Carbohydrate Metabolism
Edited yb WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited yb RONALD W. ESTABROOK AND MAYNARD E. PULLMAN
EMULOV XI. Enzyme Structure
Edited yb C. H. W. HIRS
EMULOV XlI. Nucleic Acids (in two volumes)
Edited yb NAMSSORG ECNERWAL DNA KIVlE EVADLOM
EMULOV XlII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
In Preparation
EMULOV XVI. Fast Reactions
Edited yb HTENNEK NITSUK
VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb HERBERT TABOR AND CELIA WHITE TABOR
VOLUME XVIII. Vitamins and Coenzymes (Parts AI B, and C)
Edited yb DLANOD B. McCoRMICK DNA LEMUEL D. WRIGHT
XXX