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About Lab Report CRISPR Sample 1
This lab report summarizes an experiment investigating the efficacy and mechanism of the CRISPR-Cas9 gene editing system in E. coli bacteria. The experiment uses blue-white screening to observe the function of lacZ gene in E. coli in response to independently varying whether the gene is cut by Cas9 and whether the cut can be repaired. The results show that to observe lacZ knockout, both guide RNA and enzymes for homology-directed repair must be present. Additionally, the Cas9 enzyme reliably cuts the lacZ gene when guided by RNA, confirming the mechanism of CRISPR-Cas9.
Detailed Information
| Author: | ['mayagal1707'] |
|---|---|
| Publication Year: | 2024 |
| Pages: | 9 |
| Language: | English |
| Format: | |
| Price: | FREE |
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