Table Of Content士汀YJ市出品頃hA物研けLm内九L-キ仏ゆ広凸誌。
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Karyotype Analysis of Six Species of the Genus Ophiopogon
(Convallariaceae-Ophiopogoneae)
Jun YAMASHITA and Minoru N. TAMURA
Botanical Gardens，Gr aduate School of Science，Os aka City University，
Kisaichi 2000，Ka tano-shi，Os aka，57 6-0004J APAN
(Received on October 26，2 000)
Ophiopogon brev伊esfrom Doi Suthep，C hiang Mai Prov.，T hailand，O. jaburan
from Isls. Koshikijima and Isl. Kuroshima，K agoshima Pref.，Ja pan，O. planiscapus from
Mt. Sanjohgatake，N ara Pref.，Ja pan，a nd O. siamensis from Doi Inthanon，C hiang Mai
Prov.，Th ailand were found to be diploid with 2n =3 6，o. sylvicola from Mt. Wawushan，
Sichuan，C hina to be triploid with 2n = 54，O. iαrponicus var. japonicus from Minoo，
Osaka Pref.，Ja pan to be hypotetraploid with 2n =7 0，an d O. iαrponicus var. umbrosus
from Yao，Os aka Pref.，Ja pan to be tetraploid with 2n =72 . O. brevipes，O. siαmensis d
加
O. sylvicola were karyologically investigated for the first time in the present study. The
chromosome number 2n = 54 and 2n = 70 are new counts for the genus. The karyotypes
analyzed in the present study ecategorized into two groups: Jaburan-group with two
紅
pairs of medium-sized chromosomes and Brevipes-group with three pairs of medium-
sized chromosomes，al though the karyotypes of O. japonicus ecategorized into neither
訂
group. O. jaburan，O. planiscapus，O. siamensis and O. sylvicola with karyotypes of出e
Jab uran-group，紅edistributed in warm temperate to cool tempera臼 zones.O. brevipes
with ak aryotype of the Brevipes-group，is distributed in at ropical monsoon zone.
Key words: Chromosome number，C onvallariaceae，k aryotype，L iliaceae sensu lato，
Ophiopogon
Theg enus Ophiopogon was grouped with Ophiopogon differs from Peliosanthes in
Liriope and Peliosαnthes as Convallariaceae longitudinally whitish striped leaves without
-Ophiopogoneae by Dahlgren et a .l(1985) conspicuous transverse veins，a nd flowers
and Conran and Tamura (1998)，a nd the lacking corona.
grouping was confirmed by both rbcL and Ophiopogon comprises about 55 species，
trnK molecul phylogenetic trees which are distributed in India， the
紅
(Yamashita and Tamura 2000). The three Himalayas，I ndochina，M alesia，C hina and
genera share au nique synapomorphic char- Japan. Among the 55 species，2 3 species
acteristic: the fruit rupturing in early devel- have been karyologically revealed (Table 1).
opment，ex posing the seeds with as cotesta. Chromosome numbers of the remaining
紅
They share also x= 18 basic chromosome about 32 species are still unknown. The pur-
number (Tamura 1995). Ophiopogon differs pose of the present study is to accumulate
from Liriope in perigynous actinomorphic karyological informations of the genus
flowers，ve ry short filaments，an d blue seeds. Ophiopogon in order to understand more de-
-100-
April2001 Journal of Japanese Botany Vol. 76 No. 2 101
Fig. 1. Somatic metaphase chromosomes of Ophiopogon species. A，O . planiscapus from Pref. Nara: Mt.
Sanjohgatake; B，O. japonicus var. japonicus from Osaka Pref.: Minoo. Detai1ed localities are shown in
Table 2. Bar = 5μm.
tailed karyotype diversity of the genus and to present study are given in Table 2. The
establish ab asis of more reliable classifica- plants，e xcept for O. sylvicola，w ere col-
tion of the genus. lected from their native habitats，tr ansplanted
into clay pots，a nd cultivated in Osaka City
Materials and島1ethods University. Root tip preparations were made
The sources of materials examined in the by using the squash method of Tamura
102 植物研究雑誌第76巻第2号 平成13年4月
Table 1. Present and previous karyo1ogical studies of the genus Ophiopogon
Present Previous
counts
Taxa counts References
(2n)
(n) (2n)
Ophiopogon brevipes Craib 36*
o. bockianus Diels 36 Yang et a .l(1990)
o. bodinieri Leveille 36 Yang et al. (1990)
o. chingii Wang & Tang 36 Yang et al. (1990)
o. clarkei Hook.f. 19 Sarkar et al. (1974)
38 Sarkar et al. (1974)
o. dracaenoides (Baker) Hook.f. 36 Yang et al. (1990)
o. griffithii (Baker) Hook.f. 36 Yang et al. (1990)
o. intermedius D.Don. 18 Sachdeva (1987)
(= O. aciformis Wang & Tang 36 Malik (1962)，La rsen (1963)，S harma & Chaudhuri
ex H.Li & Y.P.Yang) (1964)，S arkar et al. (1974)，R oy et al. (1988)，
(= o. wallichianus Hook工) Yang et al. (1990)
68 Sen (1973)
108 Malik (1962)，Sh eriff & Singh (1975)
112 Dudgeon (1923)
var. gracilipes Hook.f. 54 Vijayavalli & Mathew (1990a，1 990b)
108 Vijayavalli & Mathew (1990a，1 990b)
var. pauciflorus Hook.f. 54 Vijayavalli & Mathew (1990a，1 990b)
108 Vりayavalli& Mathew (1990a，1 990b)
0. jaburan (Kunth) Lodd. 36 36 Matsuura & Suto (1935)，S ato (1942)，
Hasegawa (1968)，K o et al. (1985)
o. japonicus (L.f.) Ker Gawl. 36 Sato (1942)
(= o. chekiangensis 67 Nagamatsu & Noda (1964，1 971)
K.Kimura & H.Migo) 68 Nagamatsu & Noda (1964，1 971)，
Liu et al. (1985)，G e et al. (1988)
70*
72 Sato (1942)，O inuma (1944，1 946，1 949)，S harma &
Chaudhuri (1964)，H asegawa (1968)，H su (1971)，
Ko et al. (1985)，Li ue t al. (1985)，Y ang et al. (1990)
var. umbrosus Maxim. 67 Nagamatsu & Noda (1971)
(= O. ohwii Okuyama) 68 Nagamatsu & Noda (1971)
72 72 Hasegawa (1968)，K o et al. (1985)
o. latifolius L.Rodrig. 36 Yang et al. (1990)
o. malcolmsonii Royle 36 Larsen (1963)
o. marmoratus Pierre ex L.Rodrig. 36 Yang et al. (1990)
o. megalanthus F.T.Wang & L.K.Dai 36 Yang et al. (1990)
o. micranthus Hook. f. 108 Malik (1961，1 962)
o. peliosanthoides Wang & Tang 36 Yang et al. (1990)
o. pingbienensis F.T.Wang & L.K.Dai 36 Yang et al. (1990)
o. planiscapus Nakai 18 Oinuma (1944)，H su (1971)
36 36 Oinuma (1946，1 949)，H asegawa (1968)，H su (1971)
72 Sato (1942)，O inuma (1944，1 946，1 949)
o. reptans Hook.f. 36 Larsen (1963)
o. revolutus F.T.Wang & L.K.Dai 36 Yang et al. (1990)
o. siamensis M.N.Tamura 36*
o. stenophyllus (Me凶11)L.Rodrig. 36 Yang et al. (1990)
o. sylvicola Wang & Tang 54*
0. tonkinesis L.Rodrig. 36 Yang et al. (1990)
o. tsaii Wang & Tang 36 Yang et al. (1990)
o. xylorrhizus F.T.Wang & L.K.Dai 36 Yang et al. (1990)，D ing et al. (1993)料
*These numbers were first counted.
**They may be misspe11ed O. xylorrhizus as O. sylorrhizus.
April2001 Joumal of Japanese Botany Vol. 76 No. 2 103
Table 2. Sources of materials
Taxa Localities Voucher specimens
Ophiopogon brevipes Thailand: Doi Suthep，P rov. Chiang M. N. Tamura，J. Yamashita
Mai，N ，5 20 m alt. & K. Sri-Ngemyuang 6004
O. jaburan Japan: Mt. Odake，Is ls. Koshikijima， M. N. Tamura & J. Yamashita 9905
Pref. Kagoshima，5 30 m alt.
Japan: Mt. Yaguradake，Is l. Kuroshima， J. Yamashita 3176
Pref. Kagoshima，4 00 m alt.
o. japonicus var.japonicus Japan::品1inoo，Pref. Osaka，2 90 m alt. J. Yamashita 3183
O. japonicus var. umbrosus Japan: Yao，P ref. Osaka，8 0 m alt. J. Yamashita 3016
O. planiscapus Japan: Mt. Sanjohgatake，Pr ef. Nara， J. Yamashita 3291
520 m alt.
O. siωnensis Thailand: Doi Inthanon，P rov. Chiang M. N. Tamura & J. Yamashita 6007
，
Mai，N ，1 690 m alt.
O. sylvicola China: Mt. Wawushan，H ongya， M. N. Tamura，1. Yamashita，Y. F. Yuan，
Sichuan，2 400 m alt. C. L. Liu，P. L. Wang & F. D. PU 8658
(1990). As for o. sylvicola，r oot tips were Among the 14 pairs of short chromosomes，
excised in the native habitat，a nd prepared 13 pairs have centromeres at the median re-
according to the option for Chinese plants of gion，a nd the other has centromeres at the
Tamura (1993). Nomenclature for centro- submedian region. One pair of short chromo-
meric position on chromosomes follows somes with median centromeres (the 9th
Levan et al. (1964). Voucher specimens e pair) has secondary constrictions at the cen-
紅
preserved in KYO. tral region of the short arms. This plant can
be reg紅白dkaryologically to be diploid with
x= 18 basic chromosome number.
Results 2. Ophiopogon jaburan (Kunth) Lodd.
Karyological characteristics of the six spe- (Figs. 2，6 ，T ables 4，5 )
cies and two varieties of the genus Plants collected from two localities， .i e.，
Ophiopogon examined are as follows. Is1s. Koshik討imaand Isl. Kuroshima in
1. Ophiopogon brevipes Craib (Figs. 2，6 ， Kagoshima Pref.，we re examined. They have
Table 3) the same chromosome number of 2n = 36
The chromosome number of o. brevipes is and similar karyotypes. Each karyotype is
2n = 36. The karyotype is composed of one composed of one pair of long chromosomes
pair of long chromosomes (ca. 4.8μm in (ca. 8.8μm)，t wo pairs of medium-sized
length)，th ree pairs of medium-sized chromo- chromosomes (ca. 5.7開n-ca. 5.9μm) and
somes (ca. 3.7開n-ca. 3.9μm) and 14 pairs 15 pairs of short chromosomes (ca. 2.3
of short chromosomes (ca. 2.3μm -ca. 3.2 μm- ca. 4.3μm). The pair of long chromo-
μm). The pair of long chromosomes has somes has centromeres at the median region，
cenomeresat the median region. Among and the two pairs of medium-sized chromo-
住
the three pairs of mediumsizedchromo- somes have centromeres at the submedian re-
四
somes，t wo pairs have centromeres at the gion. Among the 15 pairs of short
median region， and the other has chromosomes，o ne pair (the 6th pair) has
centromeres at the submedian region. centromeres at the subterminal region，a nd
104 植物研究雑誌 第76巻 第2号 平成13年4月
Table 3. Measurernents of sornatic rnetaphase chrornosornes of Ophiopogon brevipes
frorn Thailand: Doi Suthep. The detailed locality is shown in Table 2. rn，sr n and st
indicate chrornosornes with the centrornere in the rnedian， subrnedian and
subterminal regions，re spectively
Pair no. ぽLrenngsthm oμfm Tm。lEL---ゐdm Size Arm ratio Centrornere
目
2.3+2.5 40 L 11 m
0
2.3+2.5 40 L 11 m
0
2 1.5+2.4 3Q M 16 m
J
1.5+2.4 3Qノ M 16 m
3 1.3+2.4 3ヴ， M 18 m
1.4+2.4 3O M 17 m
0
4 1.7+2.1 300 M 12 m
1.6+2.0 3ぷU M 13 m
5 1.3+2.1 3λ斗 s 16 m
1.3+1 .7 3A s 13 m
U
6 1.5+1.7 34 s 11 m
L
1.5+1.7 3うん s 11 m
7 1.5+1.6 31 s 11 m
1
1.5+1.6 311 s 11 m
8 1.3+1 .7 3Aυ s 13 m
1.2+1.8 3ハU s 15 m
9 (0.7 +0.6)+ 1.8 3Z且 s 14 m
(0.6+0.6)+ 1.6 20 s 13 m
0
・・・4AハU 1.3+1.6 201 s 12 m
1.2+1.6 20 s 13 m
0
11 0.9+2.0 2Q s 22 m
J
1.0+1 .7 2'勺 s 17 m
12 1.2+1.6 2o s 13 m
o
1.1+1.7 2o s 15 m
O
13 1.2+1.4 2ぷU s 12 m
1.2+1.5 2I『 s 13 m
14 1.3+1.4 2勺J s 11 m
1.2+1.4 2ぷU s 12 m
15 1.2+1.4 2ぷU s 12 m
1.2+1.4 2ζU s 12 m
16 1.2+1.4 2υ兵 s 12 m
1.2+1 .4 2ζU s 12 m
17 1.2+1.4 2ぷU s 12 m
1.2+1.4 2ζU s 12 m
18 1.0+1.3 23司 s 13 m
1.0+1.2 2今中 s 12 m
the others have centromeres at the median or x= 1 8.
submedian region. One pair of short chromo- 3. Ophiopogon japonicus v japonicus
紅 .
somes with median centromeres (the 10th (L.f.) Ker Gaw .l(Figs. 1，3 ，6 ，Ta bles 6，7)
pair) has secondary constrictions at the Different two cells of the same individual
proximal region of the short arms. These collected from Minoo，O saka Pref. were ex-
plants can be regarded to be diploid based on amined. The chromosome numbers of the
April2001 Jourt1al of Japanese Botany Vo .l76 No. 2 105
It
i 持 " I
A 警 護 差 益 義 襲 撃 事 事 ね 襲 撃 襲 撃 警 警
2 3 4 5 6 7 8 9 10 11 12
議襲 撃警 護憲 議義 憲襲 撃警
13 14 15 16 17 18
“"“
81111'111"
11 " 11 .1
2 3 4 5 6 7 8 9 10 11 12
挙運護憲襲撃馨襲撃事懇馨
13 14 15 16 17 18
c 議 盆 盤 轟
蓮華 墨竃審 離器準 轟饗唖骨 轟轟響 総轄柵轡 轟轟閣胃 傘還難暢 畿郵轡嚇 盛事 畿難事 総醸柵曹 嘩龍也軍 畿轟蜘噛 畿盤叩嚇 轟難聴 機轟叩V 錨鵬響 織灘轡 畿圏曹 組事鎗事
2 3 4 5 6 7 8 9 10 11 12
藤襲撃毒事襲撃襲撃襲撃事 .
.
13 14 15 16 17 18 .
Fig. 2. Serial rangementsof somatic metaphase chromosomes of Ophiopogon species. A，O . brevipes
紅
from Thailand，D oi Suthep; B，O . jaburan from Kagoshima Pre ，.fIsls. Koshikijima; C，O. jaburan from
Kagoshima Pref.，Is l. Kuroshima. Detailed localities are shown in Table 2. Bar = 5μm.
both cells are identical，2 n = 70. The karyo- constrictions at the proximal region of the
type is composed of two p泊rsof long chro- long訂ms. This plant can be reg紅ded
mosomes (ca. 7.4阿n-ca. 8.8μm)，f ive karyologically to be hypotetraploid based on
pairs of medium-sized chromosomes (ca. 4.9 x= 18.
仰n-ca. 5.9阿n)and 28 pairs of short chro- 4. Ophiopogon japonicus var. umbrosus
mosomes (ca. 1.5阿n-ca. 4.5μm). The two Maxim. (Figs. 4，6 ，Ta ble 8)
pairs of long chromosomes have centromeres Chromosome number of the plant exam-
at the median region. Among the five pairs ined is 2n = 72. The karyotype is composed
of medium-sized chromosomes，t wo pairs of one pair of long chromosomes (ca. 8.1
have centromeres at the submedian region， μm)，f ive pairs of medium-sized chromo-
and the others have cenomeresat the somes (ca. 3.7μm-ca.5.2μm) and 30 pairs
住
subterminal region. Among the 28 pairs of of short chromosomes (ca. 1.4μm- ca. 3.4
short chromosomes，1 4 pairs have cen位0・ 阿n).The p泊rof long chromosomes has
meres at the median region，m ost of the re cenomeresat the median region. Among
“ 佐
maining 14 pairs have centromeres at the the five pairs of medium-sized chromo-
submedian region，a nd a few pairs have somes，o ne pair has cenomeresat the me-
佐
centromeres at the subterminal region. One dian region， other three pairs have
pair of short chromosomes with submedian centromeres at the submedian region，an d the
centromeres (the 9th pair) has secondary remaining one pair has cenomeresat the
住
106 植物研究雑誌 第76巻 第2号 平成13年4月
Table 4. Measurements of somatic metaphase chromosomes of Ophiopogon jaburan
from Kagoshima Pr e，.fIsl. Koshikijima
Pair no. aLnvγi戸し略目由旧owrin mTotドaml Size Arm ratio Centromere
3.8+4.0 78 L 11 m
1
3.5+3.7 72 L 1' m
1
2 1.7+3.6 53 M 22 m
1
1.7+3.6 53 M 21 m
1
3 1.4+4.1 55 M 2A m
Y
1.2+3.3 45 M 200 m
4 1.5+2.7 42 s 100 m
1.5+2.6 41 s 1f勺 m
5 1.5+2.5 40 s 1I勺 m
1.2+2.4 36 s 2AU m
6 0.5+2.8 33 s 5ζU u
0.7+2.6 33 s 3I司 は
7 0.9+2.2 31 s 2A斗 m
1.0+2.3 33 s 22 m
J
8 1.3+1 .9 32 s 1ζ m
J
1.2+1.7 29 s 1A7品 m
9 1.4+1.5 29 s 11 m
1
1.4+1.6 30 s 11 m
1
EI唱ハU (0.9+0.4)+ 1.6 29 s 1L今 m
(0.9+0.5)+ 1.5 29 s 1ti m
11 1.0+1 .7 27 s 1f勺 m
1.0+1.8 28 s 1O m
O
12 0.7+1.9 26 s 2f勺 m
0.7+2.0 27 s 2n m
y
13 0.9+1.7 26 s 1n m
y
1.0+1.7 27 s 1I勺 m
14 1.3+1 .4 27 s 1tl m
1.2+1.4 26 s 1今ゐ m
15 1.2+1 .4 26 s 1&司， m
1.2+1.3 25 s 111 m
16 0.7+1.8 25 s 2ζU m
0.8+1.8 26 s 23司 m
17 1.2+1.3 25 s 111 m
1.1+ 1. 3 24 s 1B今， m
18 1.1+1.2 23 s 111 m
1.1+ 1. 2 23 s 1'I m
subterminal region. Among the 30 pairs of constrictions at the proximal region of the
short chromosomes， 16 pairs have long arms. This plant can be regarded as a
centromeres at the median region，o ther 12 te佐aploidwith x = 18 basic chromosome
pairs have centromeres at the submedian re- number.
gion，a nd the remaining two pairs have 5. Ophiopogon planiscapus Nakai (Figs.
cenomeresat the subterminal region. One 1，4 ，6 ，T able 9)
住
pair of short chromosomes with submedian Chromosome number of the plant exam-
centromeres (the 9th pair) has secondary ined is 2n = 36. The karyotype is composed
April2001 Journal of Japanese Botany Vol. 76 No. 2 107
Table 5. Measurements of somatic metaphase chromosomes of Ophiopogon jaburan
from Kagoshima Pre ，.fIsl. Kuroshima
Pair no. um、。。:ica 白旧om Tmol・也』・凶φdm Size Arm ratio Centromere
5.0+5.7 mY L 11 m
4.5+5.0 虫5 L 11 m
2 2.2+4.2 ι4 M 19 m
2.3+4.3 ι6 M 19 m
3 1.7+4.7 ι4 M 28 m
1.7+4.7 ι4 M 28 m
4 1.6+3.1 47 s 19 m
1.3+2.8 41 s 22 m
5 1.7+2.5 42 s 15 m
1.7+2.7 44 s 16 m
6 0.8+3.3 41 s 41 は
0.9+2.8 17 s 31 は
7 1.3+2.2 15 s 17 m
1.1+2 .5 16 s 23 m
8 1.3+2.0 13 s 15 m
1.4+1.9 13 s 14 m
9 1.5+1 .7 12 s 11 m
1.5+1.7 12 s 11 m
10 (0.8+0.7)+ 1.7 12 s 11 m
(0.7+0.5)+ 1.7 Z9 s 14 m
11 1.1+2.1 12 s 19 m
1.0+1 .8 Z8 s 18 m
12 0.8+2.3 11 s 29 m
0.8+2.2 10 s 28 m
13 1.2+1.9 11 s 16 m
1.2+1.8 10 s 15 m
14 1.4+1.6 10 s 11 m
1.4+1 .7 11 s 12 m
15 1.3+1.6 Z9 s 12 m
1.4+1.6 10 s 11 m
16 1.1+1.7 z8 s 15 m
1.1+1.7 z8 s 15 m
17 1.2+1.4 z6 s 12 m
1.3+1.4 z7 s 11 m
18 0.9+1.2 z1 s 13 m
1.1+1.4 z5 s 13 m
of one pair of long chromosomes (ca. 5.6 Among the 15 pairs of short chromosomes，
阿n)，two pairs of medium-sized chromo- 10 pairs have cen佐omeresat the median
somes (ca. 4.1μm-ca.4.3阿n)and 15 pairs region，a nd the others have centromeres at
of short chromosomes (ca. 2.0μm -ca. 3.1 the submedian region. One pair of short
戸n).The pair of long chromosomes has chromosomes with median cen位omeres(the
cenomeresat the median region. The two 7th pair) has secondary constrictions at the
住
pairs of medium-sized chromosomes have proximal region of the long ms.This plant
紅
cen位omeres at the submedian region. can be regarded as ad iploid based on x= 18.
108 植物研究雑誌 第76巻 第2号 平成13年4月
Table 6. Measurements of somatic metaphase chromosomes of Ophiopogon japonicus
V紅.japonicus from Osaka Pre ，.fMinoo
M -d Total
Pair no. m Size Arm ratio Centromere
m mm mμm
42+54 96 L 13 m
4A上4lh7 91 L 11 m
2 3353F+34・}L86 7833 LL 1112 mm
3 1844h:2 60 M 23 m
4 111821・++445hlh..142 556969.••••••MMM••..•.••233•371•••...••••mはは•••••••
5 1ユ+4l 2 54 M 35 託
67 11111318213+上F+++433433hIh--lh.08408 5555435229 MMMMM 32133129356 mm前託は
119810 1111111nMAA2nM2+++++++3rti132・・・21nu4739599++ L4今，今・1u'l 、‘，、，rJ 44544347704791 sssssss 23222227619947.•••••....•mdMmmmmm•••••....•••••.....••••.....•••••.•...••••••
12 111AM3n+++222732 333762 sss 212823 mmm
13 0M+26 34 s 36 は
0S+25 32 s 39 坑
14 0Y+23 31 s 28 m
18+20 31 s 11 m
15 11+26 31 s 11 m
16 115AA++1166 3300 ss 11A1 mm
1A+15 29 s 11 m
17 11+18 29 s 16 m
13+17 30 s 13 m
18 09+18 27 s 20 m
19 100nMY后++++1122912 222988 sss 1339073 mdM
20 12+16 28 s 15 M
2212 111013389++++till7559 22228887 ssss 11122241 mmmm
09+-9 28 s 21 m
23 o9+19 28 s 20 m
o1+18 27 s 25 m
24 -1+16 27 s 15 m
-Y+16 21 s 16 m
2265 oooo998nM++++11118776 2222A5后后 ssss 21129905 mmmm
27 lA+15 25 s 16 m
223333819302 --1111101000M2222.DM9nM988凸+++++++++++++1111111111111644331111233 22222222222255jinMil--后后必 ssssssssssss 11111111111124LITAI121366 mmmmmmmmmmmmm
01+11 18 s 16 m
34 05+10 15 s 20 m
0后+10 1后 s 1Iマ m
35 0Y+08 15 s 1t- m
0后+08 14 s 1-J m
April2001 Joumal of Japanese Botany Vo .l76 No. 2 109
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襲撃襲撃善事譲事襲撃襲撃護毒事襲撃襲撃襲 撃襲 撃事
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襲撃襲撃事懇暴挙毒警襲撃襲撃奪車聾襲 撃撃事事
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Fig. 3. Serial a汀angementsof somatic metaphase chromosomes of Ophiopogon species. D，E. O. japonicus
var. japonicus from Osaka Pref.，M inoo (D and Ew ere obtained from the different cells of the same in-
dividual). Bar = 5μm.
6. Ophiopogon siamensis M.N.Tamura ined is 2n = 54. The karyotype is composed
(Figs. 5，6 ，Ta ble 10) of one pair of long chromosomes (ca. 7.3
The chromosome number of o. siamensis 仰n)，two pairs of medium-sized chromo-
is 2n = 36. The karyotype is composed of somes (ca. 5.1阿n-ca. 5.3仰n)and 15 pairs
one pair of long chromosomes (ca. 8.2凹n)， of short chromosomes (ca. 1.9μm- ca. 3.8
two pairs of medium-sized chromosomes 仰n).The pair of long chromosomes has
(ca. 5.5開n-ca. 6.1戸n)and 15 pairs of centromeres at the median region. The two
short chromosomes (ca. 2.4μm -ca. 4.8 pairs of medium-sized chromosomes have
阿n).The pair of long chromosomes has cen佐omeres at the submedian region.
centromeres at the median region. The two Among the 15 pairs of short Chromosomes，
p泊rsof medium-sized chromosomes have nine pairs have cen住omeresat the median
centromeres at the submedian region. region，a nd the others have centromeres at
Among the 15 pairs of short chromosomes， the submedian region. One pair of short
nine pairs have cenomeresat the median chromosomes with submedian centromeres
佐
region，a nd the others have cenomeresat (the 4th pair) has secondary constrictions at
佐
the submedian region. This plant can be re- the proximal region of the long紅官1S.This
g紅白das ad iploid based on x= 18. plant can be reg紅白dkaryologically to be
7. Ophiopogon sylvicola Wang & Tang triploid based on x= 18.
(Figs. 5，6 ，T able 11)
Chromosome number of the plant exam-
