Download Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis PDF Free - Full Version
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About Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis
Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!
Detailed Information
| Author: | Susanne Brakmann, Kai Johnsson |
|---|---|
| Publication Year: | 2002 |
| ISBN: | 3527301550 |
| Pages: | 359 |
| Language: | English |
| File Size: | 5.881 |
| Format: | |
| Price: | FREE |
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