Table Of ContentTHUG & HSP70 BA MSE S PCR BM
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FoR PGI bel CLEANER. EERE EMRE T LACH,
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RRS, ico ESA A aR, soba: 2
Cloning and quantitative competitive PCR assay of HSP70 gene in
Tribolium castaneum (Coleoptera; Tenebrionidae)
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Thivesiy, Zherehon ASTOR, Chim, 2. Cellegm of Plat Pre tinn, Howat Agr alie Urine,
Zoeagans 45002, Ubina)
Abwtract: To sh the mp-nsinn vk pee ol lk sek price 0-4 highly eorero pic?
‘stoem eastanaun ater cxposue te Fest ses, ¢feyment of 681 bp ereodng for PTD was apitcd
aml uel Tron Ts caswuny "The Fv reid! 227 wn voids wit te CoB,
swceeion 15, UMR4S48, The result of bomelogy avlsis shee tant this fagneatebarod 5 dency
Sits 70 Toe 2. sweat (APBEOO1LT). Ghagraon af toe ealacd anit ail seers
ISIO mt, enstanauo wth three ie Caarrso eecomfcente, Mamaxto brent, Shwe
velonos wih Liriweyoe wave ilisal il they aout ewe Uo JAKE iene. "The ‘aoa
‘ampoticr wed o qoantian comet PCR ns oni hy RT HK, Tho dnostior peony 70
a ceased by AGH aap: asi Ue es que aol AINA a ss ote aera
oto true, The iva mye of atl gucon wae PL f22%= LAR {08 fh 9993. Thie
furly povides 2 vety couroaiect acta Jv the quacilioa of the Mp7 capression camapse Ja
cs al le he asi sas kr the pret ol ont ting eral aed tele
Kes veords; Zreliom eastnawe; ect aboek protein 725 quamitere compote PUIG: ‘oteroal
contr Ienlagsaasie
WAEEEAAGAR SN PMA TUR, SST] AI | ALLER IACI. So RULER
#. AAO SBR ORY Re, SO SMAD, CMR A
RU WES EHIOS. 72, 78, TET D ES Be, HIRAI, LAT HNO AR,
ACTEN, USITO HRA T AEC cbapoonss). RAEI, SLUR BERRA
STURN SESE RERE ACHE OML VET LALIT) TET. WLM PPR, WTA Fe Wal
Hes Dabb son) Rank, 2007, AGRA]. LATA, RUE aS
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eae. Bets el ESAS EE, Fl
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atte me.
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an DFE sense se
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(TEASER, 208),
SR ASMA. AE TEP eee
Bh BIRT ATT Ha MBIA REA
MNS AACE HEP LAK Tl,
INA ARLE Tal AL, MUTA PU
FERPA. Tish Wo
UGE ALS Nonlern BA SADAESS
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TIGA, AATEC AES, 20057
SET ELE POR Caption va mpation PER, QC
PIR) Rae PC a oust sh bee
AES. NMEA ARGH, AAR EMR
SION, Sh, CORE FMM,
TERRA. gba Bree CTCF, 2an, TT
Fost Pane T. ReHLAC HUA Ee
EE, LAS Id RS he
RR. ADT, ARAMA. DE
So, BR LEADE PCR 8h
TURK E MG, MILER RLS lo
AUTREMUTIEATS, TCIR ARAL Bes Hsp 70 |p
UE, BRE ARLE RE 70 ETE LPC A
PRON. SURO TA LCL GAR,
WREM SERRE UG Aid RMA, IT
aR PS Ue fH UL a ds
POORER: scnoASeML
1 SAR
La st
NA AEA AA MRC Pe
FOBHLARCACE. THER LATC, ACSA
759% Sse Pet A Tia: Baa)
A.2 WRAL: Weel WORE Ceo JST, EEF
ARAL AS OMRE F503, Tay ples DA EAH
(Saoe 2 a5 ANT, Saga
Hg Sang: 2s)
0
12 pe
Lan eae
1. POW FB ah
SEE ATP Rie Lis
HINA AL oC
Ae site PR a a9
PAO nin, RARE eS ARERR, EOE
ERR TALS ol dist, HI Trl GF
HEU NS
12.2 GAL aera POR Pa
FRNA 2H. AT WT rem! Fat Std DNA
Ssulia IG. Gok DOA BL, TUL ait
RAMEY fap H Rh, POW Rede Bet
OM UAEEEE S vin ARAM 20 TRIS. EG
946 1 in, SS¥ Lin, FAL AS wing AES 72
SAIN ir, RMR TORR H, HEEL
ADDS Rentink RB MLE SH
{Malt ot ay 2059 4 Sgon Ad
5 AP: § TECACAGGAAATCAPTTETA #7, AP 5
SICACAATACCAAAGATCEATE, PUR AREAL,
IH San Al HS ANAL aS,
12k LACH! ALS POR AOE AL
ECEMT wos IB { Pronegn Comp, Waka, TT,
TSO ESATA Dn, HU Fou
eee
12d SEAM PO IPA, STILL Hid
ARMIN SOU, SION PTL, Elbe
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ASLLEHHIL RE OWA HN ay 1
FAW, 80%. 842, 32%, 16,8
12S AGRIC RAS ICH GT HRS
SL A OR A Ror OIA CLI
ET RSA ARERR ARR, sh
SPO, PCI PARE CUATAEN Sh EH,
CSOT Pe MSL. A SE RS a
sain SHE PCH RSA AAR, SH aS
2 BRSae
2.1 aRHG2E MNYTO AEH ABE
ORME 5 POR YSERA
Sebi. HOARSE Poteau 1h dh
Re eAh ULE FDS RE TEE, 700 bp ih
HOUSE RE NERD] LRA A)
OSHS yO HUI,
2.2 USP" SRR ASH
HEM, BRIS Js MMSIORD 34
Feo, SHU 681 I, SEALIME 2,
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Pralictios sf Trmasentrae Regions ard Orenision
sath Goulash 1H let te a MNAALAARAURE HSPTO AS AT
REL Hhg TOW FATE MS FO IZ KIRGAEBSLE
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GAEEO IES B,C, CAL
SST FE POR MALLS
Sk, RAUL Sova ema ss
FMR 89S NEA POUT.
Fa POR PN, HRI TLL 1100 bp BY
RATNER PCR MTR HR,
S80 sae Sal yet ey amet
2.4 SeAHE PCR ABI HNP ROMA HE POR A
smote
PAGE Se MAL AE
UNA AR) whi aity PO, GASSES
stot, RASA RUN TURE
A. SEAM 0 Sink, de TW HIT 100
ln A eve ARID, BAS: PCH AIL
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EMA TS IE OCHO RSE ROE
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SRW Pea AeA (Caper oo, 1987s Daugsna
sl, 2007) LRU an FLAVA ACRE Le HE
wr. SRE. Jt RE
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Hip. 6 Ue ao QL,
DS 2T REE UNA we ahs Se AKER
oa ato, 2. 1616 eS OU ELS
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Fig 7 The atsbal ref cmt PER,
Tie ASLAM Raab oc od 2008) Ha
SCRE ASAELAE Pero sara SAD Sg |
RDS ALARA MRE 1722-250
GARD, gesM Ad [At 3 FEF Kota al Tallaonte
Bosoraks, 2009), 970 19 RAITT
MER PDD PE. WLULZ USPS ERENT
See, AEN ABR TNO
PL chee ta XM) LAGIE
Tisai Peak KC NAHE. APP ATT
SPIO < ILS a WEN". EAGLE
Aca ic hae ELE. eT RSE,
BEEP EIEIO G07 HEL, Sea
SALARY. HERE MALE Mia
AREAS hi LRA (2 989%
Bio, EAU ATA Fa APE
FAL, BELL UREN RS
CGaalbnk aS, ANTOOEUD AE SY ge TEE
TWEELG, WS. LE lee OE,
ey AOC onlank BE, AYTHEOG (8 EARLE
ite 2 FEMME 6 Bin 6 RES KA 28)
ARRBAPIRES fap7OE Cena Bk HSI
FA AOE 7 Fel HE SOY IR 6 -
18640 ME, SREP NEPA RR 1
Ho hyp70 Wag sRSt ach,
ES ATL AL oh BAAS Tea
ARG. UMA TLR DRA R
BARD RNA FEAL J RCBSE ML PCR, Nonhom
inving? 4° TER Ria BTR. APE
ARTEL TOR RASS TRG
2h arb as, SLE RST TMH
er Hist, MART. Aes MAMA GD
BOWL, RGR NU BRAM A
OBR) LAE, by CREAT A, BEAT
HOUSE ARACC Cot Reem), oe
KREG nC feb abort, DNA AAA, Here
Abeta BILE DNA SAH
(Ties EIR EL CARMA MERC ITSE T,
2000). TRAE HH RIE LT 100 bp)
APrHGE: 681 bp). SAF IEA Et
SHOE 400 bp, AICTE eae dc eB
WUT. DIRT DEEN TSI0 CRANE
HER RAIUAIEULT 4 RE SOROS
DW. Jes SIODE, PeORHETEAIRD
Ae SNE REP ARAL SLATER VIR KE
Pr EAT Go OTE A
45.25). WEE, ETRE AOE
AAG, MSMR OSA AR BS
SENSE RT ALAR p70 1 81 bp JV, Ia
SET SEP UK NES. WET ARIA
HUSTON EER ACR LORRI, RATE
SOLA ZI ay SAL
ita,
ep
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an DFE sense se
ft Kamy , tea (. Sihe AE. Steaie
re
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Letra UL LAR SEP. GAP Dee. FONE
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TIN 2. LARAAENOT EM, UTA
aa
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ern nt
