By P. VISHAL KUMAR BVSc. & AH Thesis Submitte

150 Pages·2016·2.01 MB·English

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EVALUATION OF SEMEN CHARACTERISTICS AND FERTILITY IN
NELLORE SHEEP (JODIPI)
By
P. VISHAL KUMAR
B.V.Sc. & A.H

Thesis Submitted to the
SRI VENKATESWARA VETERINARY UNIVERSITY
In partial fulfilment of the requirements
For the award of the degree of

MASTER OF VETERINARY SCIENCE
In the Faculty of Veterinary Science

DEPARTMENT OF VETERINARY GYNAECOLOGY
AND OBSTETRICS
COLLEGE OF VETERINARY SCIENCE
SRI VENKATESWARA VETERINARY UNIVERSITY
TIRUPATI – 517 502

SEPTEMBER, 2014
CERTIFICATE
P. Vishal Kumar has satisfactorily prosecuted the course of research
and that the thesis entitled “EVALUATION OF SEMEN
CHARACTERISTICS AND FERTILITY IN NELLORE SHEEP (JODIPI)”
submitted is the result of original research work and is of sufficiently high
standard to warrant its presentation to the examination. I also certify that the
thesis or part thereof has not been previously submitted by him for a degree of
any university.

Date : Chairman of Advisory Committee
Place : Tirupati (DR. K. VEERA BRAMHAIAH)
CERTIFICATE
This is to certify that the thesis entitled “Evaluation of semen
characteristics and fertility in Nellore sheep (Jodipi)” submitted in partial
fulfillment of the requirements for the degree of Master of Veterinary Science
of the Sri Venkateswara Veterinary University, Tirupati, is a record of the
bonafide research work carried out by P.Vishal Kumar under my guidance
and supervision. The subject of the thesis has been approved by the Student’s
Advisory Committee.
No part of the thesis has been submitted by the student for any other
degree or diploma. The published part has been fully acknowledged. All
assistance and help received during the course of the investigations have been
duly acknowledged by the author of the thesis.
Chairman of the Advisory
Committee
Thesis approved by the Student’s Advisory Committee
CHAIRMAN : Dr. K. Veerabramhaiah _________________
Professor
Department of Veterinary
Gynaecology and Obstetrics
College of Veterinary Science
Tirupati – 517 502

MEMBER : Dr. K. Mouli Krishna ________________
Professor & Head
Department of Veterinary
Gynaecology and Obstetrics
College of Veterinary Science
Tirupati – 517 502

MEMBER : Dr.A.Ravi _________________
Professor
Department of Animal Nutrition
College of Veterinary Science
Tirupati – 517 502

MEMBER : Dr.B.Ekambaram _________________
Principal scientist and Head
Livestock Research station
Palamaner, 517 408
Chittoor dist.
LIST OF CONTENTS
Chapter Page
Title
No. No.
I Introduction
II Review of literature
III Materials and Methods
IV Results
V Discussion
VI Summary
Literature cited
LIST OF ILLUSTRATIONS
FIG.
NAME OF THE ILLUSTRATION
No
1 Photograph of Nellore Jodipi Ram
2 Photograph of Nellore Jodipi Ewe
3 Photograph of Artificial vagina parts
4 Photograph of Semen collection from Nellore Jodipi ram
5 Microphotograph of Nellore Jodipi live and dead sperms
6 Microphotograph of Nellore Jodipi pear shaped head abnormality
7 Microphotograph of Nellore Jodipi micro head sperm abnormality
8 Microphotograph of Nellore Jodipi sperm bent tail abnormality
Microphotograph of Nellore Jodipi sperm bent and coiled tail
9
abnormality
Microphotograph of Nellore Jodipi sperm with detached tail
10
abnormality
11 Microphotograph of Nellore Jodipi sperm intact acrosome
12 Microphotograph of Nellore Jodipi sperm with detached acrosome
13 Microphotograph of Nellore Jodipi sperm with damaged acrosome
14 Microphotograph of Nellore Jodipi sperm with damaged acrosome
15 Photograph of Artificial Insemination equipment
Photograph of cervical insemination in Nellore Jodipi ewe at
16
detected estrus
Proportion of individual motility of Nellore Jodipi ram in fresh
17 semen, TCFY diluted semen (0 h) and chilled semen stored at 5⁰C
(24 and 48 h)
Proportion of viability of Nellore Jodipi ram in fresh semen, TCFY
18
diluted semen (0 h) and chilled semen stored at 5⁰C (24 and 48 h)
Proportion of normal sperm percentage of Nellore Jodipi ram in
19 fresh semen, TCFY diluted semen (0 h) and chilled semen stored at
5⁰C (24 and 48 h)
Proportion of sperm abnormalities of Nellore Jodipi ram in fresh
20 semen, TCFY diluted semen (0 h) and chilled semen stored at 5⁰C
(24 and 48 h)
Proportion of acrosome integrity of Nellore Jodipi ram in fresh
21 semen, TCFY diluted semen (0 h) and chilled semen stored at 5⁰C
(24 and 48 h)
Proportion of fertility in Nellore Jodipi ewes after insemination
22 with TCFY diluted semen (0 h) and chilled semen stored at 5⁰C (24
and 48 h)
LIST OF TABLES
TABLE PAGE
TITLE
NO. NO.
Mean (±SE) semen characteristics of Nellore Jodipi ram
1
before and after dilution with TCFY dilutor
Mean (±SE) values of semen characteristics of TCFY
2 diluted semen (0 h) and chilled semen stored at 5⁰C (24
and 48 h) in Nellore Jodipi rams
Analysis of variance of Individual Motility of
3 spermatozoa in Nellore Jodipi ram TCFY diluted semen
(0 h) and chilled semen stored at 5⁰C (24 and 48 h)
Analysis of variance of live sperms in Nellore Jodipi
4 ram TCFY diluted semen (0 h) and chilled semen stored
at 5⁰C (24 and 48 h)
Analysis of variance of normal sperms in Nellore Jodipi
5 ram TCFY diluted semen (0 h) and chilled semen stored
at 5⁰C (24 and 48 h)
Analysis of variance of head abnormalities in Nellore
6 Jodipi ram TCFY diluted semen (0 h) and chilled semen
stored at 5⁰C (24 and 48 h)
Analysis of variance of mid piece abnormalities in
7 Nellore Jodipi ram TCFY diluted semen (0 h) and chilled
semen stored at 5⁰C (24 and 48 h)
Analysis of variance of tail abnormalities in Nellore
8 Jodipi ram TCFY diluted semen (0 h) and chilled semen
stored at 5⁰C (24 and 48 h)
Analysis of variance of acrosome integrity in Nellore
9 Jodipi ram TCFY diluted semen (0 h) and chilled semen
stored at 5⁰C (24 and 48 h)
Fertility rate in Nellore ewes after cervical insemination
10 of TCFY diluted semen (0 h) and chilled semen stored at
5⁰C (24 and 48 h)
ACKNOWLEDGMENTS

I wish to record my sincere and heartfelt regards to
Dr.K.Veerabramhaiah, Professor, Major advisor and Chairman,
Dr.K.Moulikrishna, Professor and Head and Member student`s advisory
committee, Department of Veterinary Gynaecology and Obstetrics, Dr.A.Ravi,
Professor, Department of Animal Nutrition, College of Veterinary Science,
Tirupati and Dr.B.Ekambaram, Principal scientist and Head Livestock
Research Station, Palamaner for their inspiring and affectionate guidance,
during the course of my work..
I avail this opportunity to express my hearty thanks to Dr. M.Srinivas,
Assoc. Professor & Head, TVCC, Tirupati, Dr.A.Teja, Assistant Professor of
this department, Dr.M.M.Rajesh, Farm manager, Livestock Research Station,
Palamaner, Dr.Selvaraju, Sr. Scientist, NIANP, Bangalore, and
Dr.Sri.G.Padmanabham, Sri.P.Jagannath Reddy, Sri.Y.Pavani, Kalyan,
Smt.Geetha Reddy, Sri.Edukondalu, Sri.Hemkumar and Sri.Ramalakshmi
PG students of this department and Sri.K.Siva Sagar Reddy, PhD student of
Veterinary Physiology for their co-operation during my work. My special
hearty thanks to all of my friends and juniors whose company brought
pleasure for me during my study.
I affectionately acknowledge the help and cooperation received from
non-teaching staff Smt.V.Syamala Devi, Sri. M. Chandrasekhar,
Smt.K.Rajyalakshmi, Sri.B.Ankaiah, Sri.J.Anand, Sri.Chengalrayudu,
Sri.Ramana and Smt.Dhanamma Department of Veterinary Gynaecology
and Obstetrics, College of Veterinary Science, Tirupati and Harikrishna,
Sudharsan Reddy, Divya, Yeshwanth, Polytechnic students and other farm
workers of LRS, Palamner.
Finally, I wish to extend my heartfelt gratitude to my father
Sri.P.Venkateshwarlu and my mother Praphulla Kumari. I am also
privileged to extend my sincere thanks to brother P.Prashanth, Grand
Parents and Relatives for their moral support.

P.Vishal Kumar
DECLARATION
I, P.Vishal Kumar, hereby declare that the thesis entitled
“EVALUATION OF SEMEN CHARACTERISTICS AND FERTILITY IN
NELLORE SHEEP (JODIPI)” submitted to Sri Venkateswara Veterinary
University for the Degree of Master of Veterinary Science is a result of
original research work done by me. It is further declared that the thesis or any
part thereof has not been published earlier in any manner.

Date : Signature of the candidate
Place : Tirupati (P.VISHAL KUMAR)
Name of the Author : P.Vishal Kumar
Title of the thesis : EVALUATION OF SEMEN CHARACTERISTICS
AND FERTILITY IN NELLORE SHEEP (JODIPI
Degree to which it is : Master of Veterinary Science
Submitted
Faculty : Faculty of Veterinary Science
Department of Veterinary Gynaecology and
Department :
Obstetrics, College of Veterinary Science, Tirupati -
517502.
Guide : Dr. K. Veera bramhaiah
Professor
University : Sri Venkateswara Veterinary University, Tirupati.
Year : september, 2014
ABSTRACT
In this study the semen characteristics immediately after collection,
after dilution (0 h) and after chilling the semen at 5⁰C for 24 and 48 h
including fertility rates after cervical insemination were studied.
The overall mean ejaculate volume, mass activity (0-5 scale), sperm
concentration in Nellore Jodipi sheep was 0.62±0.03 ml, 3.98 ± 0.08 and
4037.66 ± 42.75 million per ml, respectively. While, the mean percentage of
individual motility, live spermatozoa, normal sperm, head, mid piece and tail
abnormalities and intact acrosomes in fresh semen was 70.75 ± 0.53, 63.22 ±
0.48, 93.83 ± 0.30, 1.02 ± 0.11, 0.10 ±0.04, 5.08 ± 0.22 and 95.47 ± 0.20,
respectively.
The average mean percentage of sperm individual motility, percentage
of live spermatozoa, normal spermatozoa, head, mid piece and tail
abnormalities and intact acrosomes in Nellore ram semen extended with
TCFY dilutor (0 h) was 69.33 ± 0.51, 63.12 ± 0.40, 93.72 ± 0.23, 1.09 ± 0.12,
0.13 ± 0.04, 5.19 ± 0.16 and 95.18 ± 0.14, respectively. The difference in the
mean percentage of above parameters between fresh semen and diluted semen