Antigen Retrieval Immunohistochemistry Based Research and Diags - S. Shi, C. Taylor (Wiley, 2010) WW

470 Pages·2010·15.24 MB·English

ANTIGEN RETRIEVAL
IMMUNOHISTOCHEMISTRY
BASED RESEARCH AND
DIAGNOSTICS

WILEY SERIES IN BIOMEDICAL ENGINEERING AND
MULTI-DISCIPLINARY INTEGRATED SYSTEMS

Kai Chang, Series Editor

Advances in Optical Imaging for Clinical Medicine Nicusor Iftimia,
William R. Brugge, and Daniel X. Hammer

Antigen Retrieval Immunohistochemistry Based Research and
Diagnostics Shan-Rong Shi and Clive R. Taylor

ANTIGEN RETRIEVAL
IMMUNOHISTOCHEMISTRY
BASED RESEARCH AND
DIAGNOSTICS

Edited by

SHAN-RONG SHI

CLIVE R. TAYLOR

A JOHN WILEY & SONS, INC., PUBLICATION

1

Copyright © 2010 John Wiley & Sons, Inc. All rights reserved.

Published by John Wiley & Sons, Inc., Hoboken, New Jersey.
Published simultaneously in Canada.

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Library of Congress Cataloging-in-Publication Data:

Antigen retrieval immunohistochemistry based research and diagnostics / [edited by]
Shan-Rong Shi, Clive R. Taylor.
p. cm.—(Wiley series in biomedical engineering and multi-disciplinary integrated
systems. ; 1)
Summary: “An antigen is a substance that prompts the generation of antibodies and can
cause an immune response. The antigen retrieval (AR) technique is used worldwide and
has resulted in a revolution in immunohistochemistry (IHC). Featuring contributors who are
distinguished experts and researchers in the field, this book discusses several scientific
approaches to the standardization of quantifiable IHC. It summarizes the key problems in
the four fields of antigen retrieval and provides practical methods and protocols in AR-IHC.
Clinical pathologists, molecular cell biologists, basic research scientists, technicians, and
graduate students, will benefit from this fully up-to-date work”—Provided by publisher.
Summary: “This book is based on the development and application of AR by the editors,
one of whom is the inventor of AR, together with members of a world-leading research center
of AR”—Provided by publisher.
ISBN 978-0-470-62452-4 (hardback)
1. Immunohistochemistry. 2. Antigens. I. Shi, Shan-Rong, 1936– II. Taylor, C. R.
(Clive Roy)
QR183.6.A577 2010
616.07'56–dc22

2010024561

Printed in Singapore

10 9 8 7 6 5 4 3 2 1

v

CONTENTS

PREFACE ix
CONTRIBUTORS xv

PART I
RECENT ADVANCES IN ANTIGEN
RETRIEVAL TECHNIQUES AND
ITS APPLICATION
1

1 STANDARDIZATION OF ANTIGEN RETRIEVAL
TECHNIQUES BASED ON THE TEST BATTERY
APPROACH 3
Shan-Rong Shi and Clive R. Taylor

2 EXTENDED APPLICATION OF ANTIGEN RETRIEVAL
TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU
HYBRIDIZATION 25
Shan-Rong Shi and Clive R. Taylor

3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED,
PARAFFIN-EMBEDDED TISSUE BASED ON THE ANTIGEN
RETRIEVAL PRINCIPLE
47
Shan-Rong Shi and Clive R. Taylor

PART II
STANDARDIZATION OF
IMMUNOHISTOCHEMISTRY 73

4 KEY ISSUES AND STRATEGIES OF STANDARDIZATION
FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY
75
Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor

vi
CONTENTS

5 STANDARDIZATION OF IMMUNOHISTOCHEMISTRY
BASED ON ANTIGEN RETRIEVAL TECHNIQUE
87
Shan-Rong Shi and Clive R. Taylor

6 STANDARD REFERENCE MATERIAL: CELL LINE
DEVELOPMENT AND USE OF REFERENCE CELL
LINES AS STANDARDS FOR EXTERNAL QUALITY
ASSURANCE OF HER2 IHC AND ISH TESTING
101
Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker,
Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim,
and Keith Miller

7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS
123
Steven A. Bogen and Seshi R. Sompuram

8 STANDARD REFERENCE MATERIAL: PROTEIN-
EMBEDDING TECHNIQUE AND DESIGN OF
BAR CODE
141
Shan-Rong Shi, Jiang Gu, and Clive R. Taylor

9 THE PROS AND CONS OF AUTOMATION FOR
IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE
OF THE PATHOLOGY LABORATORY
151
David G. Hicks and Loralee McMahon

10 IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY
165
Alton D. Floyd

PART III
TISSUE/CELL SAMPLE PREPARATION
187

11 TISSUE CELL SAMPLE PREPARATION: LESSONS
FROM THE ANTIGEN RETRIEVAL TECHNIQUE
189
Shan-Rong Shi and Clive R. Taylor

12 MECHANISMS OF ACTION AND PROPER USE OF
COMMON FIXATIVES
195
Richard W. Dapson

13 CELL SAMPLE PREPARATION FOR CLINICAL
CYTOPATHOLOGY: CURRENT STATUS AND FUTURE
DEVELOPMENT 219
Yan Shi and Patricia G. Wasserman

14 DESIGN OF A TISSUE SURROGATE TO EXAMINE
ACCURACY OF PROTEOMIC ANALYSIS
235
Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O’leary

CONTENTS vii

PART IV MOLECULAR MECHANISM OF
ANTIGEN RETRIEVAL TECHNIQUE
251

15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED
ANTIGEN RETRIEVAL
253
Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O’leary

16 A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL
287
Steven A. Bogen and Seshi R. Sompuram

17
PH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL
SOLUTION: A POTENTIAL ROLE FOR REFOLDING
DURING HEAT TREATMENT
303
Shuji Yamashita

18 COMMENTARY: FUTURE DIRECTIONS
323
Timothy J. O’Leary, Carol B. Fowler, David L. Evers,
Robert E. Cunningham, and Jeffrey T. Mason

PART V
PROTEOMIC ANALYSIS OF PROTEIN
EXTRACTED FROM TISSUE/CELLS
333

19 TECHNIQUES OF PROTEIN EXTRACTION FROM
FFPE TISSUE/CELLS FOR MASS SPECTROMETRY
335
Carol B. Fowler, Timothy J. O’Leary, and Jeffrey T. Mason

20 APPLICATION OF SHOTGUN PROTEOMICS TO
FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES
347
Brian M. Balgley

21 VISUALIZING PROTEIN MAPS IN TISSUE
369
Masahiro Mukai and Mitsutoshi Setou

22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND
PROTEOMICS: MARCHING TO A NEW ERA
391
Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor

APPENDIX RELATED LABORATORY PROTOCOLS
399

INDEX 413