Table Of ContentANTIBODY PRODUCTION IN MAN
In Vitro Synthesis and Clinical Implications
ACADEMIC PRESS RAPID MANUSCRIPT REPRODUCTION
Proceedings of the Catharijne Conference
on immunology held in Utrecht, Holland
on March 15-18, 1978.
ANTIBODY PRODUCTION IN MAN
In Vitro Synthesis and Clinical Implications
edited by
ANTHONY S. FAUCI
Clinical Physiology Section
Laboratory of Clinical Investigation
National Institute of Allergy and Infectious Diseases
National Institutes of Health
Bethesda, Maryland
and
RUDY BALLIEUX
Clinical Immunology
University Hospital
Utrecht, Holland
ACADEMIC PRESS New York San Francisco London 1979
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Library of Congress Cataloging in Publication Data
Main entry under title:
Antibody production in man.
Proceedings of a workshop held in Utrecht, Holland,
Mar. 15-18, 1978.
Includes index.
1. B cells—Congresses. 2. Immunoglobulins—
Congresses. 3. Human cell culture—Congresses.
4. Immune response—Regulation—Congresses.
5. Immunological deficiency syndromes—Congresses.
I. Fauci, Anthony S. II. Ballieux, R. E.
[DNLM: 1. Antibody formation—Congresses. QW575 C361a
Library of Congress Cataloging in Publication Data
1978]
QR185.8.L9A47 616.07'93 79-928
ISBN 0-12-249950-6
PRINTED IN THE UNITED STATES OF AMERICA
79 80 81 82 9 8 7 6 5 4 3 2 1
CONTENTS
Contributors ix
Preface xiii
1 Consideration of Antibody Deficiency Diseases in Relation to
Normal B-Cell Development 1
M. D. Cooper, E. R. Pearl, and A. R. Lawton
2 Pokeweed Mitogen-Induced Responses of Human Peripheral
Blood Lymphocytes: Regulation of B-Cell Triggering 17
Anthony 5. Fauci and Barton F. Haynes
3 Induction of Antibody Synthesis in Human Lymphocytes from
Different Organs by Different PBA Substances 35
B. Rynnel-Dagöö, Ί. Kunbri, O. Ringden, and E. Möller
4 The Plaque-Forming Cell Response of Human Blood Lympho-
cytes . Cocultivation of Lymphocytes from Different Individuals 49
Marc E. Weksler, Abby Schwartz, Jay Blum, and
Young Tai Kim
General Discussion Mitogen-Induced B-Cell Activation. I. 61
Moderator: Göran Möller
5 T—T Interactions in the Generation of Human Suppressor
Effector Cells in Vitro 69
Samuel Broder, Linda Muul, Mary Durm, Carolyn Goldman,
Dean Mann, and Thomas A. Waldmann
6 Biosynthesis and Secretion of Immunoglobuhns by Human
Peripheral Blood Lymphocytes 85
Yong Sung Choi, F. Kingsley Sanders, and Robert A. Good
VI Contents
7 Antigen-Specific and Antigen-Nonspecific Triggering of Human
B Lymphocytes 101
Raif S. Geha and Fuad B. Mudawwar
General Discussion Mitogen-Induced B-Cell Activation. II. 115
Moderator: Göran Möller
8 Antigen-Induced Regulation of the PFC Response in Man 121
Hans-Michael Dosch and Erwin W. Gelfand
9 Human B-Cell Activation in Vitro: Regulation by Antigen-
Specific Human Suppressor T Cells 141
F. UytdeHaag, CobiJ. Heijnen, C. H. Pot, and R. E. Ballieux
10 Primary in Vitro Antibody Response to TNP-PAA: Cellular
Basis 159
P. Galanaud, J. F. Delfraissy, and J. Dormont
General Discussion Antigen-Induced B-Cell Activation. I. 167
Moderator: Erwin W. Gelfand
11 T-B Collaboration in the in Vitro Ab Response to Diphtheria
Toxoid: Evidence Favoring a Role for B Cells in the Generation
ofT-CellHelp 173
T. Platts-Mills, G. Oldham, E. G. de la Concha, and
M. J. Snajdr
12 Antigen-Dependent PFC Induction in Cultures of Human
Peripheral Blood Lymphocytes 185
A. L. Luzzati
13 Human Antigen-Specific Helper T-Cell Factors in B-Cell
Responses 193
James N. Woody, Ann Rees, Nathan Zvaifler, Sarah Howie,
Aftab Ahmed, Michael Strong, Robert J. Hartzman, Fred
Kantor, and Marc Feldmann
General Discussion Antigen-Induced B-Cell Activation. II. 209
Moderator: Erwin W. Gelfand
Contents νπ
14 Clinical Relevance of Subpopulation of Human T
Lymphocytes 215
L. Moretta, D. Santoli, A. Moretta, M. C. Mingari,
P. Perlmann, and H. Koprowski
15 Further Studies on the IgM Receptors Located on the Surface
of B Cells 223
M. Ferrarini, Giovanna Viale, and Elisabetta Cosulich
16 Distinct Human T-Cell Subpopulations Regulating the
Antigen-Induced Antibody Response 231
Cobi J. Heijnen, F. UytdeHaag, I. Dollekamp, and
R. E. Bailieux
General Discussion Subpopulations of Lumphocytes Involved in
the in Vitro Regulation of B-Cell Function. I. 253
Moderator: Max D. Cooper
17 Generation of Plaque-Forming Cells: Allogenic Effect and
Significance of Monocyte Removal 259
Nicholas Chiorazzi, Manlio Ferrarini, Ghodrat Montazeri,
Thomas Hoffman, and Shu Man Fu
18 Regulation of Human B-Cell Differentiation by T-Cell
Subclasses 273
Steven M. Friedman, Oscar H. Irigoyen, and Leonard Chess
19 Effect of Hydrocortisone on the Kinetics and Function of
Peripheral Blood Immunoregulatory Cells in Man 291
Barton F. Haynes, Paul Katz, and Anthony S. Fauci
General Discussion Subpopulations of Lymphocytes Involved in
the in Vitro Regulation of B-Cell Function. II. 303
Moderator: Max D. Cooper
20 In Vitro Functional Heterogeneity of Humoral and Cellular
Immune Deficiency States 309
Erwin W. Gelfand and Hans-Michael Dosch
Vlll Contents
21 Immoglobulin Secreting Cells in the Circulating Blood
and Mitogen-Stimulated Cultures from Patients with
Immunodeficiency Diseases 325
R. Michael Blaese, E. Clinton Lawrence, Andrew V.
Muchmore, Jane Grayson, Nancy J. Dooley, and
Irma R. Koski
22 Polyclonal B-Cell Activation in the Analysis of
Immunodeficiency States 333
C. Möller and C. Fernandez
General Discussion Clinical Implications in the Study of in Vitro
B-Cell Function. I. 345
Moderator: Anthony S. Fauci
23 Aberrant Regulation of B-Cell Function in Immunologically
Mediated Diseases: Systemic Lupus Erythematosus and
Sarcoidosis 351
Paul Καίζ, Barton F. Haynes, and Anthony S. Fauci
24 The Cellular Defeat in IgA Deficiency: Possible Links to
Hypogammaglobulinemia 367
T. A. E. Platts-Mills, G. Oldham, J. T. Cassidy,
A. D. B. Webster, and E. G. de la Concha
25 Subpopulation of Human T Lymphocytes: Functional
Characteristics 379
L. Moretta, M. C. Mingari, A. Moretta, G. W. Canonica, and
M. D. Cooper
General Discussion Clinical Implications in the Study of in Vitro
B-Cell Function. II. 389
Moderator: Anthony S. Fauci
Summary of Workshop Human B-Cell Function: Recent
Advances, Unanswered Questions,
and Future Directions 393
Anthony S. Fauci and Rudy E. Ballieux
Index 397
CONTRIBUTORS
Numbers in parentheses indicate the pages on which authors' contributions begin.
AFT AB AHMED (193), Department of Immunology, Naval Medical Re-
search Institute, Bethesda, Maryland
R. E. BALLIEUX (141, 231), University Children's Hospital, Het Wilhel-
mina Kinderziekenhuis, and University Hospital, Utrecht, The Nether-
lands
R. MICHAEL BLAESE (325), Cellular Immunology Section, Metabolism
Branch, National Cancer Institute, National Institutes of Health,
Bethesda, Maryland
JAY BLUM (49), The Department of Medicine, Cornell University Medical
College, New York
SAMUEL BRODER (69), Metabolism Branch, National Cancer Institute,
National Institutes of Health, Bethesda, Maryland
G. W. CANONICA (379), Istituto di Microbiologia, University ofGenova,
Genova, Italy
J. T. CASSIDY (367), The Arthritis Division, University Hospital, The Uni-
versity of Michigan, Ann Arbor, Michigan 48109
LEONARD CHESS (273), Department of Medicine, College of Physicians
and Surgeons, Columbia University, New York
NICHOLAS CHIORAZZI (259), The Rockefeller University, New York
YONG SUNG CHOI (85), Sloan-Kettering Institute for Cancer Research,
Rye, New York
E. G. DE LA CONCHA (173, 367), Centro Especial "Ramon Y. Cajal,"
Apartado 37, Madrid 34, Spain
M. D. COOPER (1, 379), The Cellular Immunobiology Unit of the Tumor
Institute, Departments of Pediatrics and Microbiology and the Com-
prehensive Cancer Center, University of Alabama, Birmingham,
Alabama
ELISABETTA COSULICH (223), Istituto di Chimica Biologica, Cattedra
di Immunologia Clinica, Universita di Genova, Genova, Italy
J. F. DELFRAISSY (159), Universite Paris-Sud and INSERM U 131, Höpi-
tal Antoine Beclere, Clamart, France
I. DOLLEKAMP (231), University Children's Hospital, Het Wilhelmina
Kinderziekenhuis, Utrecht, The Netherlands
ix
