Table Of ContentANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS
JAACT’97 NAGOYA
JAACT’97 Organizing Committee
Chairperson:
Yasuo KITAGAWA Nagoya University BioScience Center
Secretary General:
Tsukasa MATSUDA School of Agricultural Sciences, Nagoya University
Program Organizer:
Shinji IIJIMA School of Engineering, Nagoya University
Advisory Boards:
Takeshi KOBAYASHI School of Engineering, Nagoya University
Atsushi KAKINUMA School of Agricultural Sciences, Nagoya University
Shio MAKING School of AgriculturalSciences, Nagoya University
Norihiro TSUKAGOSHI School of Agricultural Sciences, Nagoya University
Organizing Boards:
Koji UCHIDA School of Agricultural Sciences, Nagoya University
Masamichi KAMIHIRA School of Engineering, Nagoya University
Kiyotaka HITOMI School of Agricultural Sciences, NagoyaUniversity
Fumihiko HORIO School of Agricultural Sciences, Nagoya University
Hiroyuki HONDA School of Engineering, Nagoya University
Tatsuo MURAMATSU School of Agricultural Sciences, Nagoya University
Hiroaki ODA School of Agricultural Sciences, NagoyaUniversity
Ken-ichi NISHIJIMA School of Engineering, Nagoya University
Kiyoshi MIKI Nagoya University BioScience Center
Katsuhide MIYAKE School of Engineering, Nagoya University
Ken KITAJIMA School of Agricultural Sciences, Nagoya University
Naohito AOKI School of Agricultural Sciences, Nagoya University
Program Committee:
Masuo AIZAWA Tokyo Institute of Technology
Toshihiro AKAIKE Tokyo Institute of Technology
John BARFORD University of Sydney
Shun'ichi DOSAKO Snow Brand Milk Products Co., Ltd
Hiroyoshi HOSHI Research Institute of Functional Peptides
Wei-Shou HU University of Minnesota
Koji IKURA Kyoto Institute of Technology
David BARNES Oregon State University
David JAYME Life Technologies Inc.
Shuichi KAMINOGAWA University of Tokyo
Kin-ichi KAWAMURA Komatsugawa Chemical Engineering Co., Ltd.
Yasuo KITAGAWA Nagoya University
Yoji MITSUI Nat. Inst. Biosci. Human-Tech.
Alan MOORE Microbiological Associates Inc.
Tadao OHNO Riken Gene Bank
Ralf PORTNER TechnischeUniversitat, Hamburg-Harburg
Ryuzo SASAKI Kyoto University
Kazuo SHIN Meiji Milk Products Co. ,Ltd.
Haiime UNNO Tokyo Institute of Technology
John WERENNE Universite Libre de Bruxelles
Animal Cell Technology:
Basic & Applied Aspects
Volume 10
Proceedings of the Tenth Annual Meeting of the
Japanese Association for Animal Cell Technology,
Nagoya, Japan, November 5–8, 1997
Edited by
Y. KITAGAWA
Bioscience Center, Nagoya University,
Nagoya, Japan
T. MATSUDA
Department of Applied Biological Sciences,
Graduate School of Agricultural Sciences,
Nagoya University, Nagoya, Japan
and
S IIJIMA
Department of Biotechnology,
Graduate School of Engineering,
Nagoya University, Nagoya, Japan
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Contents
Preface xiii
Symposium 1
Role of Heparan Sulfate Proteoglycan in Cell Growth and
Differentiation
THE FUNCTION OF DALLY, A DROSOPHILA GLYPICAN, IN DPP
SIGNALING
Nakato, H., Izumi, S. and Selleck, S. B. 1
Symposium 2
Glycoengineering
NOVEL FORMS OF PROTEIN O-GLYCOSYLATION
Haltiwanger, R. S. 7
SYNTHESIS OF GLYCOCONJUGATES: THEIR USE TO STUDY THE INTRACELLU-
LAR TRAFFIC OF ENDOGENOUS LECTINS AND TO DELIVER
OLIGONUCLEOTIDES AND GENES
Monsigny, M.,Quetard,C.,Bourgerie,S.,Carriere,V.,Duverger,E.,Altemayer,V., 15
Pichon, C., Mayer, R., Midoux, P. and Roche, A. C.
OLIGO/POLYSIALIC ACIDS IN SEA URCHIN: A DEVELOPMENTALLY REGULATED
SEAURCHIN POLYSIALYLTRANSFERASE
Cho, J. W. 23
CLONING AND APPLICATION OF GLYCOSYLTRANSFERASES INVOLVED IN THE
BIOSYNTHESIS OF SELECTIN LIGANDS
Sasaki, K., Kurata-Miura, K., Hasegawa, M. and Nishi, T. 27
INDUCTION OF GANGLIOSIDE BIOSYNTHESIS, NEURITE OUTGROWTH AND
FUNCTIONAL SYNAPSE FORMATION BY A SYNTHETIC CERAMIDE ANALOG
Inokuchi, J. 33
Symposium 3
Artificial Organ and Cell Therapy
HUMAN CYTOTOXIC T LYMPHOCYTES FOR TUMOR THERAPY
Ohno, T. 37
ORGANOID FORMATION WITH BRANCHED CAPILLARY-LIKE NETWORKS
Takezawa, T. and Yoshizato, K. 43
v
vi
Symposium 4
Immunological Manipulation
CYTOTOXIC ANTIBODIES TO ADIPOCYTES: AGRICULTURAL AND CLINICAL
POTENTIAL
Kestin, S., Futter, C. E., Tonner, E., Kennedy, R.., Butterwith, S., Rhind, S. M., 49
Griffin, H., Cryer, A. and Flint, D. J.
IMMUNOLOGICAL MANIPULATION OF LACTATION
Wilde, C. J., Addey, C. V. P., Campbell, F. M. and Peaker, M. 55
LIGHT CHAIN SHIFTING-CHARACTERIZATION OF LIGHT CHAIN REPLACE-
MENT IN HUMAN PLASMA B CELLS
Haruta, H., Tachibana, H. and Yamada, K. 63
Symposium 5
DT40 Cells as a System of Homologous Gene Recombination
CREATION OF HYBRIDOMAS FROM MICE EXPRESSING HUMAN ANTIBODY BY
INTRODUCTION OF A HUMAN CHROMOSOME
Yoshida, H., Tomizuka, K., Uejima, H., Satoh, K., Ohguma, A., Oshimura, M. and 69
Ishida, I..
Symposium 6
Transgenic Livestock
TRANSGENICALLY PRODUCED BIOPHARMACEUTICALS
Young, M. W. 75
OPTIMISATION OF TRANSGENE EXPRESSION IN THE MAMMARY GLAND
Whitelaw, C. B. A. and Clark, A. J. 81
Symposium 7
Transgenic Fish: Standardization of Technology in the Model
System and Aquaculture
INTRODUCTORY REMARK: A DECADE OF TRANSGENIC FISH
Ozato, K. 91
TRANSGENIC MEDAKA FISH STRAINS WITH LIGHTENED BODY COLOR AND
BIOLUMINESCENCE
Kinoshita, M. 93
vii
TRANSGENIC ZEBRAFISH AS A BIOSENSOR
Yamashita, M. 97
TRANSGENIC SALMON FOR AQUACULTURE
Fletcher, G. L. and Hew, C. L. 101
Symposium 8
Growth, Immortality and Death of Cells in Culture: Present and
Future Impact fortheDevelopmentofHealth Care Products
FOOD COMPONENTSWITH POTENTIALITIES TO SUPPRESS PROLIFERATION
AND INVASION OF CANCER CELLS
Yagasaki, K. and Miura, Y. 107
DEVELOPMENT OF THROMBOPOIETIN: ITS STRUCTURE AND FUNCTION
Ohgami, K. 113
Symposium 9
Gene Transfer to Cells and Tissues of Living Animals: Applica-
tion to Production and Therapeutics
BACULOVIRUS NOT ONLY AS AN INSECT EXPRESSION VECTOR BUT AS A GENE
TRANSFER VECTOR
Kobayashi, J. 119
IN VIVO GENE ELECTROPORATION: MANIPULATIONS OF CELLS TO WHOLE
BODY OF LIVING ANIMALS
Muramatsu, T. 125
Oral Session
Cell Culture for Assessment of Physiologically Functional
Substances
IN VITRO STIMULATION OF TUMOR NECROSIS FACTOR PRODUCTION FROM
RAT SPLENOCYTES BY CABBAGE EXTRACT
Komatsu, W., Miura, Y. and Yagasaki, K. 131
EFFECTS OF GREEN, OOLONG AND BLACK TEAS AND RELATED COMPONENTS
ON PROLIFERATION AND INVASION OF HEPATOMA CELLS
Zhang, G. Y., Miura, Y. and Yagasaki, K. 135
Expression of Recombinant Animal Genes in Cultured Animal Cells
viii
MILK SECRETION IN CULTURED MAMMARY EPITHELIAL CELLS
Blatchford, D. R., Wilde, C. J., Matsuda, T. and Aoki, N. 141
HYPERTHERMIC INDUCTION OF GENE EXPRESSION USING STRESSINDUCIBLE
PROMOTER
Bouhon, I. A., Ito, A., Shinkai, M., Honda, H. and Kobayashi, T. 147
Cell Culture Engineering
ENHANCEMENT OF PRODUCTIVITY OF RECOMBINANT ENZYME
BY LOW TEMPERATURE CULTURE
Furukawa, K. and Ohsuye, K. 153
DIALYSIS-FIXED-BED CULTURES FOR HIGHLY EFFICIENT PRODUCTION OF
CHIMERIC FAB-FRAGMENTS WITH TRANSFECTOMA CELLS IN PROTEIN-FREE
MEDIUM
Pörtner, R.,Lüdemann, I.,Reher, K.,Neumaier, M.andMärkl, H. 159
Cost Performance for Producing Biochemicals
MONITORINGDRUGEFFECTS ON HUMANPRIMARYTISSUES UTILIZINGFIBRE
OTICAL SENSORS
Maerz, H. 165
Establishment of Functional Cell Lines for Production of Biologicals
INCREASING ANTIBODY PRODUCTION BY SUPPRESSING CELLPROLIFERA-
TION WITH CONTROLLABLE EXPRESSION OF CYCLIN INHIBITOR P21 AND P27
Fujita, T., Terada, S., Ueda, H., Nagamune, T. and Suzuki, E. 171
ESTABLISHMENT OF AN APOPTOSIS-RESISTANT AND GROWTH-CONTROL-
LABLECELLLINE BYTRANSFECTING WITH INDUCIBLE ANTISENSE c-JUN
GENE
Kim,Y. H.,Iida,T.,Prochownik,E.V.andSuzuki,E. 177
IMPROVEMENT OFCELLSURVIVAL BY INHIBITION OFCASPASES PLAYING
KEYROLESINAPOPTOSIS
Terada, S.,Yata,T.,Fukuoka, K.,Aizawa, H.,Fujita,T.,Nagamune,T.and Suzuki, 185
E.
Application of HumanMonoclonalAntibody
IN VITRO IMMUNIZATION OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES
WITH 14-16 kDa RICE ALLERGEN PROTEINS
Ichikawa, A.,Katakura,Y.,Teruya, K.,Hashizume, S.,Matsuda, T.andShirahata, S. 191
ix
Cell Characterization and Validation
OPTIONS TO ELIMINATE ANIMAL-DERIVED COMPONENTS OF CELL CULTURE
MEDIA
Jayme, D. W. and Smith, S. R.
199
CHARACTERIZATION OF THE SUBPOPULATIONS OF RAT ASCITES HEPATOMA
AH109ACELLSWITHDIFFERENTINVASIVEANDMETASTATICACTIVITIES
Miura, Y., Miyauchi, M., Kubota, K., Li, Q., Tachikawa, H., Fujimoto, D. and
Yagasaki, K. 205
FUNCTIONALEXPRESSION OFNMDARECEPTORCHANNELS INDIFFERENTIAT-
INGNEURALPRECURSORCELLS
Yamada, K., Hisatsune,T., Uchino,S., Nakamura,T.,Kudo,Y. and Kaminogawa, S. 211
INDUCESCELLSENESCENCEINADENOCARCINOMACELL
LINE
Katakura, Y.,Nakata,E.,Miura,T. andShirahata,S. 217
Poster Session
Cell Culture Engineering
EVALUATIONOFAFIXED-BEDTYPEBIOREACTOR SYSTEM USINGRECOMBI-
NANT CHOCELLS PRODUCING HUMANTRANSFERRIN
Nemoto, A.,Hirota-Iwai,Y.,Miyazaki,Y.,Nakamichi, N.,Ito,M.,Tokiwa, T., 223
Kodama, M. andMatsumura,T.
FORMATION OFCYLINDRICALMULTICELLULARAGGREGATE(CYLINDROID)
OF RATHEPATOCYTESONPRESSED SHEETOFPOLYURETHANE FOAM
Mizumoto,H., Kaneko, M.,Nakazawa, K.,Ijima,H.,Matsushita,T. andFunatsu, 229
K.
PRODUCTION OFA NOVEL FUSIONPROTEINFOR
USE INSERUM-FREEMEDIA
Simula, A. P., Barone, C.,Priebe, I. K.,Francis, G., Ballard, F.J.,Wheldrake, J. and 235
Goddard, C.
EFFECT OF THE MACROMOLECULAR METABOLISM OF MUR1NE HYBRIDOMA
CELLS ON ANTIBODY PRODUCTIVITY IN BATCH AND FED-BATCH CULTURE
Jang,J. D. andBarford,J. P. 241
EFFECT OF GLYCINE BETAINE AS OSMOPROTECTANT ON THE PRODUCTION
OF ERYTHROPOIETIN BY CHO CELLS IN HYPEROSMOTIC SERUM FREE MEDIA
CULTURE
Ahn,Y.-H.,Han, K.,Yoon,S. K. and Song, J.-Y. 247
X
Expression of Recombinant AnimalGenes in Cultured AnimalCells
ESTABLISHMENTOFRECOMBINANTCHOCELLLINESPRODUCINGWILD-TYPE
AND MUTANT HUMAN
Nakamichi, N., Shimizu, M., Ito, M., Ikai, A. and Matsumura, T. 251
EVALUATION OF STABILITY IN THE DHFR GENE AMPLIFICATION SYSTEM
USING FLUORESCENCE IN SITU HYBRIDIZATION
Nakanishi, F., Yoshikawa, T., Itami, S., Omasa, T., Katakura, Y., Kishimoto, M. and 259
Suga, K.
EXPRESSION AND CHARACTERIZATION OF HUMAN CALPAIN AND
CALPASTATIN USING BACULOVIRUS SYSTEM
Hitomi, K. and Maki, M. 265
Cell Characterization and Validation
EVALUATION OF SPIM INSECT CELLS USING A NOVEL BACULOVIRUS EXPRES-
SION VECTOR SYSTEM EMPLOYING THE HYPHANTRIA CUNEA NPV
Takenaka, Y., Kobayashi, J., Matsuda, Y., Gong, C., Nagaya, M., Miyajima, S. and 271
Yoshimura, T.
Transplantation and Artificial Organs
DEVELOPMENT OF A HYBRID ARTIFICIAL LIVER SUPPORT SYSTEM WITH
PORCINE HEPATOCYTE SPHEROIDS FOR PRECLINICAL USE
Kaneko, M., Ito, T., Nakazawa, K., Ijima, H., Matsushita, T., Gion, T., Shimada, M., 277
Shirabe, K., Hasegawa, H., Takenaka, K., Sugimachi, K. and Funatsu, K.
IN VITRO SELF-ORGANIZATION OF LIVER CELLS USING ARTIFICIAL MATRIX
Yamada, K., Kamihira, M., Hamamoto, R. and Iijima S. 283
THYROID TRANSPLANTATION: POSSIBILITY OF APPLICATION FOR THE TREAT-
MENT OF PERSISTENT HYPOTHYROIDISM AND STUDY OF MECHANISMS OF
INTERACTION BETWEEN GRAFT AND HYPOTHALAMIC-PITUITARY AXIS OF
RECIPIENT
Pasteur, I. P., Tronko, N. D., Drozdovich, I. I., Turchin, I. S. and Balla, I. A. 289
FIXED-BED REACTORS AS A TOOL FOR ARTIFICIAL ORGANS
Pörtner, R., Rössing, S., Stange, J. and Fassnacht, D. 295
Transgenic Technology
TRANSCRIPTIONAL ACTIVITIES OF VIRAL AND CELLULAR PROMOTERS IN
VIVO IN THE OVIDUCT OF LIVING CHICKENS
Park, H.-M., Okumura, J. and Muramatsu, T. 299
