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SARS- and Other Coronaviruses: Laboratory Protocols PDF

334 Pages·2008·8.986 MB·English
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About SARS- and Other Coronaviruses: Laboratory Protocols

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Detailed Information

Author:Leen Vijgen PhD, Elien Moës, Els Keyaerts PhD, Sandra Li, Marc Van Ranst MD, PhD (auth.), Dave Cavanagh (eds.)
Publication Year:2008
ISBN:9781597451819
Pages:334
Language:English
File Size:8.986
Format:PDF
Price:FREE
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