Table Of ContentHormone Binding and
Target Cell Activation
in the Testis
CURRENT TOPICS IN MOLECULAR ENDOCRINOLOGY
Series Editors: Bert W. O'Malley and Anthony R. Means
Department of Cell Biology
Baylor College of Medicine
Houston, Texas
Volume 1; Hormone Binding and Target Cell Activation in the Testis
Edited by Maria L. Dufau and Anthony R. Means
A Continuation Order Plan is available for tbis series. A continuation order will bring
delivery of each new volume immediately upon publication. Volumes are billed only upon
actual shipment. For furtber information please contact tbe publisher.
Hormone Binding anti
Target Cell Activation
in the Testis
Edited by
Maria L. Dufau
Reproduction Research Branch
National Institute of Child Health and Human Development
National Institutes of Health
Bethesda, Maryland
and
Anthony R. Means
Department of Cell Biology
Baylor College of Medicine
Houston, Texas
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
Main entry under title:
Hormone bin ding and target cell activation in the testis.
(Current topics in molecular endocrinology; v. 1)
"Proceedings of the workshop conference held at Baylor College of Medicine,
Houston, Texas, February 11-13, 1974, sponsored by the National Institute of Child
Health and Human Development and the Baylor Center for Population Research and
Reproductive Biology."
Includes bibliographical references and index.
1. Testicle-Congresses. 2. Hormones, Sex-Congresses. I. Dufau, Maria L.,
ed. II. Means, Anthony R., ed. III. United States. National Institute of Child
Health and Human Development. IV. Baylor University, Waco, Tex. College of
Medicine, Houston. Center for Population Research and Reproductive Biology.
V. Series. [DNLM: 1. Binding sites-Congresses. 2. Receptors, Hormone
Congresses. 3. Testis-Metabolism-Congresses. Wl CU821 v. 1 1974 / WJ830
B612 1974)
QP255.B54 612.6'1 74-23709
ISBN-13: 978-1-4684-2597-0 e-ISBN-13: 978-1-4684-2595-6
DOI: 10.1007/978-1-4684-2595-6
Proceedings of the Workshop Conference held at
Baylor College of Medicine, Houston, Texas, February 11-13, 1974
sponsored by the National Institute of Child Health and Human Development,
and the Baylor Center for Population Research and Reproductive Biology
© 1974 Plenum Press, New York
Softcover reprint ofthe hardcover 1st edition 1974
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y. 10011
United Kingdom edition published by Plenum Press, London
A Division of Plenum Publishing Company, Ltd.
4a Lower J ohn Street, London, Wl R 3PD, England
All rights reserved
No part of this book rnay be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
Foreword
It is curious that research in endocrinology has largely
ignored the testis until quite recently. There were two impor
tant reasons for this neglect; first, methods of study were
difficult, and second, sperinatogenesis was considered to be
the concern of the urologist or cell biologist but not the
endocrinologist. Since it is now almost an ethical imperative
that we develop a male contraceptive, and since a host of new
techniques can be brought to bear on problems of testis function,
research in male reproductive biology has effloresced. In fact,
it has become possible to project aseries of workshops on the
testis, each dealing with discrete aspects of biochemistry,
physiology and pathology.
It is fitting that this first Workshop should be on Binding and
Activation, since this area is one of the frontiers in endocrinol
ogy. At our present rate of progress it is probable that each
of the succeeding workshops will likewise bring together leaders
in a rapidly developing area. The National Institute of Child
Health and Human Development has the major Federal respon
sibility in reproductive biology, and has therefore agreed to
sponsor this and succeeding workshops. On behalf of the
Institute and for those members of the Committee who have
organized this meeting, I welcome you. I am quite sure that
this first Workshop on the Testis will initiate aseries of
important contributions to scientific thought in male reproduc
tive biology.
Mortimer B. Lipsett, M. D.
February 1974
v
Preface
The Conference which this book represents would not have
been possible without the support of Dr. Mortimer B. Lipsett
and the NICHD. We are especially grateful to Dr. Lipsett for
his central role in the organization and planning of the Confer
ence. The format of this volume follows the order of presenta
tions during the meeting, in which three basic areas are
obvious. The first is concerned with the binding of LH and hCG
to target tissues, and with some of the early metabolie actions
of these gonadotropic hormones. The second area is concerned
with gene rally similar information concerning the binding and
actions of FSH. The final topics are concerned with the binding
of steroid hormones within specific testicular cell types. This
program and format resulted from the recommendations of an
organizing committee which consisted of several of the scien
tists who served as the chairpersons of the various sessions
during the Conference. For their services we are most appre
ciative.
The editors also wish to express their sincere thanks to
the secretarial staff of the Department of Cell Biology for
making our stay in Houston a pleasant one. The participants
themselves must be commended for their timely and valuable
contributions which made possible both the conference and
this book. Finally. we owe a debt of gratitude to Ms. Terri
Sellner for the preparation and typing of the manuscripts.
and to Plenum Press for maintaining a high priority for the
volume in regard to the time of publication.
Anthony R. Means, Ph. D.
Maria L. Dufau, M. D.
August 1974
vii
Contents
1. Gonadotropin binding and activation of the interstitial
cells of the testis . • • . • • . • . . . • . . • • • • 1
Kevin Catt. Tsuneo Tsuruhara. Carole Mendelson.
Jean-Marie Ketelslegers and Maria L. Dufau
2. Computer analysis of the binding reaction between hCG
and gonadotropin receptors of the rat testis . • • • . • 31
Jean-Marie Ketelslegers. Gary D. Knott and Kevin
J. Catt
3. Characteristics of soluble gonadotropin receptors for
LH and hCG .•...••••...•..• 47
Maria L. Dufau. Eduardo H. Charreau. David W.
Ryan and Kevin J. Catt
4. Apparent positive cooperativity in the mechanism of
action of luteinizing hormone . • . • • • • . • • . • 79
David Rodbard and J. Ramanchandran
5. Functional groups in ovine luteinizing hormone and their
receptor interactions . • • . . . . . • . • • . . . • • 89
Wan-Kyng Liu. Kuo-Pao Yang. Bruce D. Burleigh
and Darrell N. Ward
6. Conformational and metabolie aspects of gonadotropins .• 109
David Puett. Mario Ascoli and Leslie A. Holladay
7. The role of carbohydrate in the biologie al function of
human chorionic gonadotropin. • . . . • • . • • • . . . . 125
Om P. Bahl. Leopold Marz and William R. Moyle
8. Coupled events in the early biochemical actions of
FSH on the Sertoli cells of the testis • • . • . • • . • •• 145
Anthony R. Means and Claire Huckins
9. The production of androgen binding protein by Sertoli
cells ..................... ,. . .. 167
Donald J. Tindall. W illiam T. Schrader and
Anthony R. Means
ix
x CONTENTS
10 FSH binding in rat testes during maturation and
0
following hypophyseetomy. Cellular loealization
of FSH reeeptors . . • . . . . . . . . . . . • . 177
Anna Steinberger, K. J. Tanki and B. Siegal
11. Binding of human FSH and its subunits to rat testis ... 193
David Rabin
12. FSH reeeptors in rat testes. Chemieal properties and
solubilization studies ..............••.. 201
V. K. Bhalla and Leo E. Reiehert
13. In Vitro binding and autoradiographie loealization of
human ehorionie gonadotropin and follicle stimulating
hormone in rat testes during development ........ 221
Claude Desjardins, Anthony J. Zeleznik, A. Rees
Midgley, Jr. and Leo E. Reiehert, Jr.
14. The effeets of FSH on eell preparations from the rat
testis . . . . . . . . . . .. • ...•......• 237
J ennifer H. Dorrington, N. F. Roller and Irving
B. Fritz
15. Evidenee for multiple, eell specifie, distinetive
adenylate eylease systems in rat testis .......... 243
Theodor Braun
16. Androgen transport and reeeptor meehanism in testis
and epididymis . . . . . . . . . . . • . . . . . . . . . • 265
Frank S. Freneh, W. S. MeLean, A. A. Smith,
Donald J. Tindall, Samuel C. Weddington, Peter
Peter Petrusz, Madha,.bananda Sar, Walter E.
Stumpf, Shihadeh N. Nayfeh, Vidar Hansson,
O. Trygstad and E. Martin Ritzen
17. FSH stimulation of testieular androgen binding protein
(ABP) . . . . . . . . . . . . . . . . . . . . . . . . • . 287
Vidar Hansson, Frank S. Freneh, Samuel Wedding
ton, Shihadeh N. Nayfeh a~d Eo Martin Ritzen
18. Properties of rat testieular androgen bin ding
propertie s . • . . . . . . . . • . . . • • . . . . . . . . 291
Barbara M. Sanborn, J ohn S. H. Elkington
and Emil Steinberger
19. Effeets of FSH on levels of androgen binding protein in
the testis. . . . . . . . . . . • • . . • . . . . .. • 311
Irving B Fritz, Bozena Kopee, Kam Lam and
o
Riehard G. Vernon
CONTENTS xi
20. Studies on the correlation between androgen binding and
activation of rat epididymal tubules in culture. . • • • . • 329
Jorge A. Blaquier. MQnica S. Cameo and Ricardo
Calandra
21. Testicular estradiol receptors in the rat ••••••••• 343
E. Mulder. W. M. O. van Beurden-Lamers. W. De
Boer. A. O. Brinkman and H. J. van der Molen
. . . .
22. Conference Summary ••.••..•..•• • • • 357
William T. Schrader and Bert O'Malley
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . 365
