Activation and Desensitization of Transducing Pathways

313 Pages·1990·8.023 MB·English

by  Melvin I. Simon (auth.), T. M. Konijn, M. D. Houslay, P. J. M. Van Haastert (eds.)

NATO ASI Series
Advanced Science Institutes Series
A series presenting the results of activities sponsored by the NATO Science
Committee, which aims at the dissemination of advanced scientific and
technological knowledge, with a view to strengthening links between scientific
communities.
The Series is published by an international board of publishers in conjunction with
the NATO Scientific Affairs Division
A Life Sciences Plenum Publishing Corporation
B Physics London and New York
C Mathematical and Kluwer Academic Publishers
Physical Sciences Dordrecht, Boston and London
D Behavioural and
Social Sciences
E Applied Sciences
F Computer and Springer-Verlag
Systems Sciences Berlin Heidelberg New York
G Ecological Sciences London Paris Tokyo Hong Kong
H Cell Biology
Series H: Cell Biology Vol. 44
The ASI Series Books Published as a Result of
Activities of the Special Programme on
CELL TO CELL SIGNALS IN PLANTS AND ANIMALS
This book contains the proceedings of a NATO Advanched Research Workshop held within the
activities of the NATO Special Programme on Cell to Cell Signals in Plants and Animals, running
from 1984 to 1989 under the auspices of the NATO Science Committee.
The books published as a result of the activities of the Special Programme are:
Vol. 1: Biology and Molecular Biology of Plant-Pathogen Interactions. Edited by JA Bailey. 1986.
Vol. 2: Glial-Neuronal Communication in Development and Regeneration.
Edited by H.H. Althaus and W Seifert. 1987.
Vol. 3: Nicotinic Acetylcholine Receptor: Structure and Function. Edited by A. Maelicke. 1986.
Vol. 4: Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions.
Edited by B. Lugtenberg. 1986.
Vol. 5: Mesenchymal-Epithelial Interactions in Neural Development.
Edited byJ.R. Wolff, J. Sievers, and M. Berry. 1987.
Vol. 6: Molecular Mechanisms of Desensitization to Signal Molecules.
Edited byTM. Konjin, P.J.M. Van Haastert, H. Van der Starre, H. Van derWel,
and MD. Houslay. 1987.
Vol. 7: Gangliosides and Modulation of Neuronal Functions. Edited by H. Rahmann. 1987.
Vol. 9: Modification of Cell to Cell Signals During Normal and Pathological Aging.
Edited By S. Govoni and F. Battaini. 1987.
Vol. 10: Plant Hormone Receptors. Edited by D. Klambt. 1987.
Vol. 11: Host-Parasite Cellular and Molecular Interactions in Protozoal Infections.
Edited by K.-P. Chang and D. Snary 1987.
Vol. 12 The Cell Surface in Signal Transduction. Edited by E. Wagner, H. Greppin, and B. Millet. 1987.
Vol. 19: Modulation of Synaptic Transmission and Plasticity in Nervous Systems.
Edited by G. Hertting and H.-G. Spatz. 1988.
Vol. 20: AminoAcid Availability and Brain Function in Health and Disease. Edited by G. Huether. 1988.
Vol. 21: Cellular and Molecular Basis of Synaptic Transmission. Edited by H. Zimmermann. 1988.
Vol. 23: The Semiotics of Cellular Communication in the Immune System.
Edited by E.E. Sercarz, F. Celada, NA Mitchison, and T Tada. 1988.
Vol. 24: Bacteria, Complement and the Phagocytic Cell. Edited by FC. Cabello and C. Pruzzo. 1988.
Vol. 25: Nicotinic Acetylcholine Receptors in the Nervous System.
Edited by F Celementi, C. Gotti, and E. Sher. 1988.
Vol. 26: Cell to Cell Signals in Mammalian Development.
Edited by SW. de Laat, J.G. Bluemink, and C.L. Mummery. 1989.
Vol. 27: Phytotoxinsand Plant Pathogenesis. Edited by A. Graniti, R.D. Durbin, and A. Ballio. 1989.
Vol. 31: Neurobiology of the Inner Retina. Edited by R. Weiler and N.N. Osborne. 1989.
Vol. 32: Molecular Biology of Neuroreceptors and Ion Channels. Edited by A. Maelicke. 1989.
Vol. 33: Regulatory Mechanisms of Neuron to Vessel Communication in the Brain.
Edited byF Battaini, S. Govoni, M.S. Magnoni, and M. Trabucchi.1989.
Vol. 35: Cell Separation in Plants: Physiology, Biochemistry and Molecular Biology.
Edited by D.J. Osborne and M.B. Jackson. 1989.
Vol. 36: Signal Molecules in Plants and Plant-Microbe Interactions. Edited by B.J.J. Lugtenberg. 1989.
Vol. 39: Chemosensory Information Processing. Edited by D. Schild. 1990.
Vol. 41: Recognition and Response in Plant-Virus Interactions. Edited by R.S.S. Fraser. 1990.
Vol. 43: Cellular and Molecular Biology of Myelination.
Edited by G. Jeserich, H. H. Althaus, and T V. Waehneldt. 1990.
Vol. 44: Activation and Desensitization ofTransducing Pathways.
Edited by T M. Konijn, M. D. Houslay, and P J. M. Van Haastert. 1990.
Activation and Desensitization
of Transducing Pathways
Edited by
T. M. Konijn
Zoblogisch Laboratorium, Kaiserstraat 63,
POB 9516,2300 RA Leiden, The Netherlands
M. D. Houslay
Institute of Biochemistry, The University
of Glasgow, Glasgow G12 800, Scotland
P. J. M. Van Haastert
Biochemisch Laboratorium, Nijenborgh 16,
POB 9747 AG Groningen, The Netherlands
Springer-Verlag Berlin Heidelberg New York
London Paris Tokyo Hong Kong
Published in cooperation with NATO Scientific Affairs Division
Proceedings of the NATO Advanced Research Workshop on Activation
and Desensitization of Transducing Pathways held at Noordwijkerhout,
The Netherlands, May 25-27,1989
ISBN-13: 978-3-642-83620-6 e-ISBN-13:978-3-642-83618-3
001: 10.1007/978-3-642-83618-3
Library of Congress Cataloging-in-Publication Data
NATO Advanced Research Workshop on Activation and Desensitization of Transducing Pathways (1989:
Noordwijkershout, Netherlands) Activation and desensitization of transducing pathways 1 edited by 1 M.
Konijn, M. D. Houslay, P. J. M. van Haastert. p. cm.-(NATO ASI series. Series H, Cell biology; vol. 44)
"Published in cooperation with NATO Scientific Affairs Division." "Proceedings of the NATO Advanced
Research Workshop on Activation and Desensitization of Transducing Pathways held at Noordwijkershout,
the Netherlands, May 25-27,1989"-Ip. verso.
Includes bibliographical references.
ISBN-13:978-3-642-83620-6 (U.S.: alk. paper)
1. Cellular signal transduction-Congresses. 2. Second messengers (Biochemistry)-Congresses. I. Konijn,
1 M. II. Houslay, Miles D. III. North Atlantic Treaty Organization. Scientific Affairs Division. IV. Haastert, P. J. M.
van. V. Title. VI. Series. QP517.C45N37 1989 674.87'6-dc20 90-9800
This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned,
specifically the rights of translation, reprinting, re-use of illustrations, recitation, broadcasting, reproduction on
microfilms or in other ways, and storage in data banks. Duplication of this publication or parts thereof is only
permitted under the provisions of the German Copyright Law of September 9, 1965, in its current version, and a
copyright fee must always be paid. Violations fall under the prosecution act of the German Copyright Law.
© Springer-Verlag Berlin Heidelberg 1990
Softcover reprint of the h<rdcover 1st edition 1990
2131/3140-543210 - Printed on acid-Iree-paper
Contents
PARTICIPANTS ix
PREFACE xi
CHEMOTAXIS AND CHEMOSENSING
Activation and desensitization in bacterial chemotaxis
M.I. SIMON 3
Discussion 18
Adaptation of chemoattractant elicited responses in
Dictyostelium discoideum
R. VAUGHAN, R. JOHNSON, M. CATERINA and P. DEVREOTES 23
Discussion 37
The inositolcycle of Dictyostelium discoideum
P.J.M. VAN HAASTERT, A.A. BOMINAAR, J. VAN DER KAAY,
R. DRAIJER, L.C. PENNING, E. ROOVERS, M.M. DE VRIES,
A.A. VINK, F. KESBEKE, and B.E. SNAAR-JAGALSKA 41
Discussion 56
CYCLIC NUCLEOTIDE-COUPLED SYSTEMS
Glucagon activates two distinct signal transduction
systems in hepatocytes,which leads to the
desensitization of G-protein-regulated adenylate cyclase,
the phosphorylation and inactivation of G-2 and the
i
phosphorylation and stimulation of a specific cyclic AMP
phosphodiesterase
M.D. HOUSLAY, M. BUSHFIELD, E. KILGOUR, B. LAVAN, S.
GRIFFITHS, N.J. PYNE, E.K.-Y. TANG and G.J. MURPHY 63
Discussion 79
Multiple pathways for glucagon-induced heterologous
desensitization of liver adenylyl cyclase
R.T. PREMONT and R. IYENGAR 85
Discussion 95
The mechanisms of luteinizing hormone-induced
activation and desensitization of adenylate cyclase
B.A. COOKE, D.R.E. ABAYASEKERA, and M.P. ROSE 99
Discussion 110
VI
Role of protein kinases in the desensitization
sensitization of ~-adrenergic receptor and
prostaglandin receptor stimulation of adenylyl cyclase
R.B. CLARK, M.W. KUNKEL, J.A. JOHNSON, R. GOLDSTEIN,
and J. FRIEDMAN 115
Discussion 129
Direct involvement of the cyclic nucleotide binding
sites in the cyclic-nucleotide-induced charge shift of
protein kinases
L.WOLFE, T.A. WOODFORD, S.H. FRANCIS, and J.D. CORBIN 133
Discussion 148
INOSITOL PHOSPHOLIPID-COUPLED SYSTEMS
Heterogeneity amongst myo-inositol derivatives:
metabolism of second messengers and synthesis of
co-signals
C.P. DOWNES, L.R. STEPHENS, and P.T. HAWKINS 153
Discussion 162
Role of glycosyl-phosphatidylinositols in insulin
signalling
I. VARELA, J.F. ALVAREZ, J. PUERTA, R. CLEMENTE, A.
GUADANO, M. AVILA, F. ESTEVEZ, S. ALEMANY, and
J .M. MATO 167
Discussion 177
Agonist and guanine nucleotide regulation of P2Y-
purinergic receptor-linked phospholipase C
J.L. BOYER, M.W. MARTIN, C.L. COOPER, G.L. WALDO,
A.J. MORRIS, H.A. BROWN, R.A. JEFFS, J.R. HEPLER, C.P.
DOWNES, and T.K. HARDEN 181
Discussion 194
G-PROTEIN ACTIVATION
Mechanisms involved in G-protein activation by hormone
receptors
K.H.JAKOBS, T. WIELAND, and P. GIERSCHIK 199
Discussion 210
VISUAL TRANSDUCTION
Regulation and rapid inactivation of the light induced
cGMP phosphodiesterase activity in vertebrate retinal
rods
M. CHABRE, P. DETERRE, P. CATTY, and T.M. VUONG 215
Discussion 226
VII
AUXIN-COUPLED SYSTEMS
Characterization and modulation of the sensitivity of
plant protoplasts to auxin
H. BARBIER-BRYGOO, G. EPHRITIKHINE, W.H. SHEN, A.
DELBARRE, D . KLAMBT, and J. GUERN 231
Discussion 243
The auxin-binding-protein from maize coleoptiles
G. VIOLA, U. TILLMANN, T. HESSE, B. KNAUTH, K. PALME,
M. L~BLER, and D. KLAMBT 245
Discussion 251
Is a GTP-binding protein involved in the auxin-mediated
phosphoinositide response on plant cell membranes?
B. ZBELL, H. HOHENADEL, I. SCHWENDEMANN, and C. WALTER-
BACK 255
Discussion 264
ION CONDUCTANCE
Regulation of the desensitization of the acetylcholine
nicotinic receptor
C. MULLE, P. BENOIT, F. REVAH, AND J.P. CHANGEUX 269
Discussion 280
CELLULAR SYSTEMS
The control of human thyroid cell function,
proliferation and differentiation
S. REUSE, C. MAENHAUT, A. LEFORT, F. LIBERT, M.
PARMENTIER, E. RASPE, P. ROGER, B. CORVILAIN, E.
LAURENT, J. MOCKEL, F. LAMY, J. VAN SANDE, G. VASSART,
and J.E. DUMONT 285
Discussion 304
Activation of the regulated secretory pathway in
neuronal cells during in vitro differentiation
E. SHER, S. DENIS-DONINI, A. ZANINI, C. BISIANI, E.
BIANCARDI, and F. CLEMENTI 307
Discussion 319
Participants
NATO Workshop on Activation and Desensitization of Transducing
Pathways, held at Noordwijkerhout, The Netherlands, 24th-27th
May, 1989.
H. BARBIER-BRYGOO, Centre National de la Recherche
Scientifique, Physiologie cellulaire vegetale, Batiment 15,
Avenue de la Terrasse, F-91190 Gif-Sur-Yvette, France.
J.L. BOYER, Dept. of Pharmacology, Univ. of North Carolina,
School of Medicine, Chapel Hill, NC 27514, USA.
M.G. CARON, Dept. of Physiology, Duke Univ. Med. Center,
Durham, NC 27710, USA.
M. CHABRE, CNRS - Institut de Pharmacologie, Route des
Lucioles, Sophia Antipolis, F-06560 Valbonne, France.
R.B. CLARK, Graduate School of Biomedical Sciences, Univ. of
Texas Health Science Center, POB 20334, Houston, TX 77225,
USA.
F. CLEMENTI, Dipartimento di Farmacologia, Universita di
Milano, Via Vanvitelli 32, 1-20129 Milano, Italy.
B. COOKE, Dept. of Biochemistry, Royal Free Hospital School of
Medicine, Rowland Hill Street, London NW3 2PF, England.
J.D. CORBIN, Howard Hughes Medical Institute, Dept. of
Molecular Physiology and Biophysics, Vanderbilt School of
Medicine, Nashville, TN 37232, USA.
P.N. DEVREOTES, The Johns Hopkins University, School of
Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205,
USA.
C.P. DOWNES, Smith, Kline & French Research Ltd, The Fryt he,
Welwyn, Hertfordshire, England AL6 9AR.
J.E. DUMONT, Faculte de Medicine, Campus Hopital Erasme, Route
de Lennik 808, B-1070 Bruxelles, Belgium.
x
G. EPHRITIKHINE, Centre National de la Recherche Scientifique,
Physiologie cellulaire vegetale, Batiment 15, Avenue de la
Terrasse, F-91190 Gif-Sur-Yvette, France
M.D. HOUSLAY, Inst. of Biochemistry, The Univ. of Glasgow,
Glasgow G12 8QQ, Scotland.
R. IYENGAR, Dept. of Pharmacology, Mount Sinai School of
Medicine, One Gustave Levy Place, New York, NY 10029, USA.
K.H. JAKOBS, Pharmakologisches Inst. der Univ. Heidelberg, Im
Neuenheimer Feld 366, 0-6900 Heidelberg, FRG.
D. KLAMBT, Botanisches Institut der Univ. Bonn, Meckenheimer
Allee 170, 0-5300 Bonn 1, FRG.
T.M. KONIJN, Zoologisch Laboratorium, Kaiserstraat 63, POB
9516, 2300 RA Leiden, The Netherlands.
K.R. LIBBENGA, Botanisch Laboratorium, Nonnenstreeg 3, POB
9516, 2300 RA Leiden, The Netherlands.
J. MATO, Metabolismo Nutrici6n y Hormonas, Fundaci6n Jimenes
Diaz, Avda de los Reyes Cat61icos 2, 28040 Madrid 3, Spain.
C. MULLE, Molecular Neurobiology, Institut Pasteur, 28 Rue du
Dr. Roux, 75724 Paris Cedex 15, France.
R. PREMONT, Dept. of Pharmacology, Mount Sinai School of
Medicine, One Gustave Levy Place, New York, NY 10029, USA.
P. SCHAAP, Zoologisch Laboratorium, Kaiserstraat 63, POB 9516,
2300 RA Leiden, The Netherlands.
M.I. SIMON, Calif. Inst. of Technology, Division of Biology
147-75, Pasadena, CA 91125, USA
B.E. SNAAR-JAGALSKA, Zoologisch Laboratorium, Kaiserstraat 63,
POB 9516, 2300 RA Leiden, The Netherlands.
P.J.M. VAN HAASTERT, Biochemisch Laboratorium, Nijenborgh 16,
9747 AG Groningen, The Netherlands.
B. ZBELL, Botanisches Institut der Universitat, Im Neuenheimer
Feld 360, 0-6900 Heidelberg, FRG.
Hostess: Eva Luderus
Projection: Cor Schoen
Recording discussions: Bert Van Duijn, Fanja Kesbeke and Ton
Bominaar
Mieke Rozenboom
Secreta~:
Preface
This book reports the proceedings of a small NATO-supported workshop
held at Noordwijkerhout, in the Netherlands, during 25-27th. May 1989.
The aim of this workshop was for a panel of experts to present data and
analyse future developments in the rapidly expanding field of signal
transduction processes in biological systems. This field will have
considerable importance for the burgeoning biotechnology and biomedical/
health-care areas, well into the 21st century. Its pervasive nature was
explored in the various representatives of the workshop, who were involved
in investigating a diverse array of creatures from micro-organisms and slime
moulds through to plants and animal cells. In all such creatures we find
that cellular signalling systems are of crucial importance in allowing such
creatures to survive, adapt, grow and differentiate. Intriguingly, the
fundamental components of the signalling systems employed show a high
degree of conservation, although their usage is clearly individually tailored to
particular creatures and Situations.
One clear observation, has been the major impact that molecular biology
has made to this field over the past few years. This has provided the nascent
beginnings of relating the fine structure of components of signal
transduction systems to their particular functions. The proteins involved in
signal transduction processes are normally found in vanishingly small
quantities, which makes for distinct difficulties in their analysis. However,
molecular biological techniques coupled with new developments in dealing
with membrane proteins should, in the future, allow us to look forward to
producing considerable quantities of these proteins for X-ray
crystallographic analysis.
We have known for some time that a diverse array ofp roteins was
involved in the various signal transduction processes. However, molecular
biological analyses have indicated that the true diversity of such systems is
far greater than we ever envisaged. The functional significance of these
observations and the mechanisms whereby signal transduction systems
interact together, to form networked arrays, will provide a conSiderable
challenge for the future.
The Editors May 1989