Shafayat Ahmad Beigh

203 Pages·2017·16.68 MB·English

STUDIES ON METABOLIC PROFILE AND OXIDATIVE
STRESS IN CROSS BRED DAIRY CATTLE
By
Shafayat Ahmad Beigh
(J-10-D-26-V)
Thesis submitted to Faculty of Postgraduate Studies
In partial fulfillment of requirements
For the degree of
DOCTOR OF PHILOSOPHY
IN
VETERINARY CLINICAL MEDICINE AND
JURISPRUDENCE
Je^fel'^.
Division of Veterinary Medicine
Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu
Main Campus, Chatha, Jammu 180009
2014
CERTIFICATE-I
This is to certify that the thesis entitled "Studies on Metabolic Profile and
Oxidative Stress in Cross Bred Dairy Cattle" submitted in partial fulfilment of the
requirements for the degree of Doctor of Philosophy in Veterinary Clinical
Medicine and Jurisprudence to the Faculty of Post-Graduate Studies, Sher-e-
Kashmir University of Agricultural Sciences and Technology of Jammu, is record of
bonafide research, carried out by Mr. Shafayat Ahmad Beigh, Registration Number
J-10-D-26-V, under my supervision and guidance. No part of the thesis has been
submitted for any other degree or diploma.
It is further certified that help and assistance received during the course of
thesis investigations have been duly acknowledged.
DrXRajivSingh
(Major Advisor)
Place: R.S.Pura, Jammu
Date: 55^- ^- ^»lt|
(Dr.'^ajivlSingh)
Heat
Division of Veterinary Medicine
Date: O&^OS- ^^^'M
CERTIFICATE-II
We, the members of Advisory Committee of Mr. Shafayat Ahmad Beigh,
Registration Number J-10-D-26-V, a candidate for the degree of Doctor of
Philosophy in Veterinary Clinical Medicine and Jurisprudence, have gone
through the manuscript of the thesis entitled "Studies on Metabolic Profile and
Oxidative Stress in Cross Bred Dairy Cattle" and recommend that it may be
submitted by the student in partial fulfilment of the requirements for the degree.
>mgh
Major Advisor & Chairman
Advisory Committee
Place: R. S. Pura, Jammu
Date: c5^S- «=>2-- SLo I 4
Advisory Committee Members
Dr. S.K. Gupta
Dean
F.V.Sc. & A.H., SKUAST-J, R.S. Pura
Dr. Rajinder Raina
Professor
Division of Veterinary Pharmacology and Toxicology
Dr. Utsav Sharma
Associate Professor and Head
Division of Veterinary Gynaecology and Obstetrics
Dr. J.S. Soodan (D E Nominee)
Professor and Head
Division of TVCC
CERTIFICATE-III
This is to certify that the thesis entitled "Studies on Metabolic Profile and
Oxidative Stress in Cross Bred Dairy Cattle" submitted by Mr. Shafayat Ahmad
Beigh, Registration Number J-10-D-26-V, to the Faculty of Post-Graduate Studies,
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, in
partial fulfilment of the requirements for the degree of Doctor of Philosophy in
Veterinary Clinical Medicine and Jurisprudence, was examined and approved by
the Advisory Committee and External Examiner on \A <k.M 9^ A /X^)M
fernal Examiner » /. /
Dr. S.N.S. Randhawa ^(?~|5| I J/
Director Research-cum-Dean PGS,
Directorate of Research, GADVASU,
Ludhiana-141 004 (Pb)
Singh
tajor Advisor
Dr. Rajiv Singh
Head
Division of Veterinary Medicine
Director Ed'ucation
CERTIFICATE- IV
Certified that all the necessary corrections as suggested by the external
examiner/evaluator and the advisory committee have been duly incorporated in the
thesis entitled "Studies on Metabolic Profile and Oxidative Stress in Cross Bred
Dairy Cattle" submitted by Mr. Shafayat Ahmad Beigh, Registration Number J-
10-D-26-V.
Dr.VRajiv Singh
Major Aavisor & Chairman
Advisory Committee
Dated: O^- o S'- 2>^l^
Division of Veterinary Medicine
ACKNOWLEDGEMENT
First off all I offer my reverences to "Almighty God", as it's all by his grace that I have
been able to complete this work.
At the outset of this epistle, I consider myself fortunate and greatly privileged to
have worked under the supervision and guidance of Dr. Rajiv Singh, Professor & Head,
Division of Veterinary Medicine, R. S. Pura Campus of SKUAST-Jammu. Words are
inadequate to express my sincere and deepest feelings of gratitude originating from the
innermost core of my heart for his kind affection, benevolent guidance, meticulous
supervision, whole hearted encouragement, critical appreciation in execution of my work
and for all the trust he had in my ability primarily responsible for the present
accomplishment.
With a delectable feeling, it is my sole prerogative to place on record my
indebtedness and everlasting gratitude to the member of my Advisory Committee Dr. S.
K. Gupta (Deam, F. V.Sc &A.H) Dr. J. S .Soodan Professor (TVCC), Dr. Rajinder Raina,
Professor (VPT), and Dr. Utsav Sharma Professor (VGO) for their help, innovative
guidance and invaluable suggestions during the course of present studies.
I am extremely grateful to Dr P.S. Brar Professor, Department of Animal
Reproduction (GADVASU), Dr P.S. Sethi Professor Animal Nutrition (GADVASU), Dr
Chanchal Singh Assistant professor Veterinary Biochemistry (GADVASU), Dr. R. Katoch
Professor (VPP) for extending their helping hand during the course of study. I also avail
this opportunity to extent thanks Dr V.S. Wazir Associate professor (VMD), DR. Kafil
Hussain, Dr. Abha Tickoo R.K.Bhardwaj, Dr. S.R.Upapadya and Dr R. Agarwal,
Assistant Professor (VMD) to for their genuine intrest, guidance and conceptual
technical ideas during the course of planning.
I also avail this opportunity to extend my special thanks to the incharge officer
military Dairy form, Satwari, Jammu, associated staff, DR. Adil and Dr. Virampal for
extending wholehearted help during my research programme
I am thankful to Dr. S. K. Gupta, Dean, F. V.Sc & A.H. for his academic support,
encouragement and providing the necessary facilities that facilitated my research
I am highly thankful to Hon 'ble Vice Chancellor of SKUAST-Jammu for allowing
me to undertake the study and for providing necessary facilities to carry out my research
work. I owe my gratitude towards Dr. MD. Saleem, Director Education for being kind
enough to provide the facilities and academic support
Thanks are duly acknowledged to the non teaching staff of Division of Veterinary
Medicine specially Sh. Balwant (Computer Assistant) Sh. Rajesh Ganjoo, Sr.
Stenographer (VMD), Sh. Kewal Krishna, FCLA (VMD), Sh. Yash, Sh. Rakesh and Sh.
Thoru and special thanks for Sh. Ajay (Computer Assistant, VGO) for their cooperation
during my entire post graduate programme.
I record with immense pleasure, my sincere thanks to my colleagues Dr. Adil, Dr.
Adarsh, Dr Ishfaq, Dr. Muneer and Dr Shuhab for their wholehearted support, constant
inspiration and for sharing the moments of laughter and sorrow during this investigation.
I am also very much thankful to Dr. Gourav, Dr. Atul , Dr Himalni who have proved
exceptionally great inspirers and true friends and whose timely help greatly helped me
during the course of study.
Words fail to describe the love and moral support I received during stay in
SKUAST-J from my juniors as well as seniors. In this perspective, I wish to exert my
eulogy and whole hearted endearment to my best friends Aadil mehraj khan and Qazi
AAmir for their extreme psychological and moral support during the times when I
required it the most.
All the words in the lexicon will be futile and meaningless, if I fail to divulge my
extreme sense of regards to my adorable parents, sister and especially brother for their
sacrifice, prayers and blessings without which this work would have been a sweet dream.
Finally once again and above all I express my gratitude to Almighty God for
giving me this life for his obedience and to serve other mankind. It is his name which will
last forever.
Needless to say, all omissions and errors are mine.
Shafayat^Akmad ^Ish
Place: R. S. Pura
Dated: ^f-^-^L*!^
ABSTRACT
Title of Thesis Studies on metabolic profile and oxidative
stress in cross bred dairy cattle.
Name of Student and Admission Shafayat Ahmad Beigh (J-10-D-26V)
no. ,
Major Subject Veterinary Clinical Medicine and Jurisprudence
Name and Designation of Major Dr. Rajiv Singh, Professor & Head
Advisor Division, of Veterinary Medicine, F.V.Sc & A.H,
R.S Pura, Jammu.
Degree to be awarded Doctor of Philosophy
(Veterinary Clinical Medicine and Jurisprudence)
Year of award of degree 2014
Name of University Sher-e-Kashmir University of Agricultural
Sciences & Technology, Jammu (J&K)
To study the metabolic profile and oxidative stress during pregnancy and
lactation, primiparous (PP) and multiparous (MP) animals (lOno each) alongwith high
(HY) and low (LY) yielders (6no each) were selected from organized farm and blood
samples were collected 8, 4, 2 and 1 week before, at calving and 1, 2, 4, 8 and 12 weeks
after parturition. Analysis of haematological parameters viz. Hb and PCV revealed
significant decline during the peri-parturient period. Dry matter intake, body condition
score (BCS), glucose, TPP, albumin, total cholesterol, HDL-C, VLDL and triglyceride
level declined and NEFA, PUN, AST, GGT, bilirubin and creatinine increased during
peri-parturient period and changes in blood parameters were strongly correlated with
milk yield and BSC. Parity of animal did not influence blood biochemical parameters
except GGT and VLDL values, however there was significant time*parity interaction.
Ca, Pi, Mg, Zn, Cu and iron levels of plasma samples were significantly lower during the
peri-parturient period with lowest concentration at calving. Calcium and phosphorus were
influenced by the parity and there was significant time*group interaction in Ca, Pi and
copper levels, however zinc level showed time*group interaction only between HY and
LY.
Evaluation of oxidative stress parameters during peri-parturient period revealed
significant increase in MDA level, SOD and GST activities along with decrease in GSH,
vitamin E and C among all category of animals. GPx and catalase activities increased
during pre-partum period and thereafter decreased in post-partum period among PP, MP
and HY animals. Oxidative stress parameters were affected by both yield and parity.
MDA level was positively correlated with BSC and NEFA. Pre-partum feeding of
UMMB improved dry matter intake, energy, protein, fat and mineral balance.
Administration of Se-vitamin E injection and UMMB supplementation during the pre-
partum period lowered the oxidative stress during peri-parturient period and improved
fertility rate.
rards: Haematological parameters, Lactation, Oxidative stress. Pregnancy, UMMB. Se-
''itamin E
Major Advisor Sign^ure^f^^
TABLE OF CONTENTS
CHAPTER TOPIC PAGE (S)
I. INTRODUCTION 1-6
II. REVIEW OF LITERATURE 7-27
III. MATERIALS AND METHODS 28-34
IV. RESULTS 35-93
V. DISCUSSION 94-121
VI. SUMMARY AND CONCLUSION 122-129
REFERENCES 130-160
VITA
LIST OF TABLES
TABLE PAGE
TITLE CHAPTER
NO. NO.
1. Chemical composition of the diet. Results 34
2. Effect of parity and time in relation to calving on -do- 37
dry matte intake and hematology in primiparous
and multiparous animals (Mean ± S.E).
3. Effect of yield and time in relation to calving on -do- 38
dry matter intake, and hematology in high and low
yielding animals (Mean ± S.E).
4. Effect of parity and time in relation to calving on -do- 41
energy balance in primiparous and multiparous
animals (Mean ± S.E).
5. Effect of yield and time in relation to calving on -do- 41
energy balance in high and low yielding animals(
Mean ± S.E).
6. Effect of parity and time in relation to calving on -do- 45
protein balance in primiparous and multiparous
animals (Mean ± S.E).
7. Effect of yield and time in relation to calving on -do- 46
protein balance in high and low yielding animals
(Mean ± S.E).
8. Effect of parity and time in relation to calving on -do- 49
lipid proflle in primiparous and multiparous
animals (Mean ± S.E).
9. Effect of yield and time in relation to calving on -do- 50
lipid profile in high and low yielding animals
(Mean ± S.E).
10. Effect of parity and time in relation to calving on -do- 53
liver function in primiparous and multiparous
animals (Mean ± S.E).
11. Effect of yield and time in relation to calving on -do- 54
liver function in high and low yielding animals
(Mean ± S.E).
12. Effect of parity and time in relation to calving on -do- 57-58
plasma minerals in primiparous and multiparous
animals (Mean ± S.E).
13. Effect of yield and time in relation to calving on -do- 59-60
plasma mineral in high and low yielding animals
(Mean ± S.E).
14. Effect of parity and time in relation to calving on -do- 66-67
oxidative stress parameters in primiparous and
multiparous animals (Mean ± S.E).