ARTI VERMA PhD SOIL Sc 2017

163 Pages·2017·3.14 MB·English

INHERITANCE OF QUANTITATIVE AND
QUALITATIVE TRAITS AND DOWNY MILDEW
RESISTANCE IN CAULIFLOWER
(Brassica oleracea L. var. botrytis)

THESIS

By

ARTI VERMA
(A-2013-40-016)

Submitted to

CHAUDHARY SARWAN KUMAR
HIMACHAL PRADESH KRISHI VISHVAVIDYALAYA
PALAMPUR – 176 062 (H.P.) INDIA
in
Partial fulfilment of the requirements for the degree
of
DOCTOR OF PHILOSOPHY IN AGRICULTURE
(DEPARTMENT OF VEGETABLE SCIENCE AND FLORICULTURE)
(VEGETABLE SCIENCE)
2017
Dr. Yudhvir Singh Department of Vegetable Science and Floriculture,
Professor & Head College of Agriculture
CSK Himachal Pradesh Krishi Vishvavidyalaya
Palampur-176 062 (H.P.) India

CERTIFICATE – I

This is to certify that the thesis entitled, “Inheritance of quantitative
and qualitative traits and downy mildew resistance in cauliflower (Brassica
oleracea L. var. botrytis)”, submitted in partial fulfilment of the requirements
for the award of the degree of Doctor of Philosophy (Agriculture) in the
discipline of Vegetable Science of Chaudhary Sarwan Kumar Himachal Pradesh
Krishi Vishvavidyalaya, Palampur is a bonafide research work carried out by
Ms. Arti Verma daughter of Smt. Suman Devi and Sh. Ramesh Kumar Verma
under my supervision and that no part of this thesis has been submitted for any
other degree or diploma.
The assistance and help received during the course of this investigation
have been fully acknowledged.

Place: Palampur (Dr. Yudhvir Singh)
Dated: February , 2017 Major Advisor
CERTIFICATE- II

This is to certify that the thesis entitled, “Inheritance of quantitative and
qualitative traits and downy mildew resistance in cauliflower (Brassica
oleracea L. var. botrytis)”, submitted by Ms. Arti Verma (Admission No. A-
2013-40-016) daughter of Sh. Ramesh Kumar Verma to Chaudhary Sarwan Kumar
Himachal Pradesh Krishi Vishvavidyalaya, Palampur in partial fulfilment of the
requirements for the degree of Doctor of Philosophy (Agriculture) in the
discipline of Vegetable Science has been approved by the Advisory Committee after
an oral examination of the student in collaboration with an External Examiner.

(Dr. Yudhvir Singh) ( )
Chairperson External Examiner

(Dr. Akhilesh Sharma) (Dr. H. K. Chaudhary)
Member Member

(Dr. D. K. Banyal) (Dr. S. C. Negi)
Member Dean‟s Nominee

__________________
Head of the Department

______________________
Dean, Postgraduate Studies
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ACKNOWLEDGEMENTS

This treatise, a humble expression of my life’s ambition and a scientific dream come true, would
have not materialized the way it is, without the benediction from almighty GOD. With limitless humility,
I am Grateful to “GOD” the merciful, the compassionate, who bestowed me with health, tenacity and
courage enough to go through this crucial juncture. I am grateful to “God”, for bestowing me with
affectionate parents, whose love, dedication & inspiration encouraged me to undergo higher studies.
This thesis is the end of my journey in obtaining my Ph.D. I have not traveled in a vacuum in this
journey. This thesis has been kept on track and been seen through to completion with the support and
encouragement of numerous people including my parents, well wishers and friends. At the end of my thesis
I would like to thank all those people who made this thesis possible and an unforgettable experience for me.
With the overwhelming sense of legitimate pride and genuine obligation which gives me exuberant
pleasure and privilege to express my eternal gratitude to my learned and revered guide Dr. Yudhvir Singh,
Professor (Vegetable Science and Floriculture), CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur
and Chairperson of my Advisory Committee. This work would not have been possible without his guidance,
support and encouragement. Under his guidance I successfully overcame many difficulties and learned a
lot. His unflinching courage and conviction will always inspire me, and I hope to continue to work with
his noble thoughts. I can only say a proper thanks to him through my future work. I can see the good shape
of my thesis because of him and his suggestions in formatting the entire manuscript.
No expression of thanks will be sufficient without recognition of intelligent and professional
dexterity of the members of my Advisory Committee; Dr. Akhilesh Sharma (Professor, Department of
Vegetable Science and Floriculture), Dr. H. K. Chaudhary (Professor, Department of Crop Improvement),
Dr. D. K. Banyal (Professor, Department of Plant Pathology) and Dr. S. C. Negi (Chief Scientist,
Department of Agronomy) for their valuable guidance and constructive suggestions.
I avail myself of this rare opportunity to express my ecstatic thanks to all the teachers of
the Department of Vegetable Science and Floriculture for their kind cooperation and impeccable
guidance during the courses of the study. Heartfelt thanks are also due to all the members of laboratory,
office and field staff of the department for their cordial assistance and timely help extended during the
study.
I take this opportunity to sincerely acknowledge the Department of Science and Technology
(DST), Government of India, New Delhi, for providing financial assistance in the form of INSPIRE
Fellowship which buttressed me to perform my work comfortably.
I can hardly overlook the co-operation, timely help, moral support extended by the galaxy of my
seniors especially Nimit Sir, Sangeeta Ma’am and Binny Di, my friends; Naiya, Preeti, Akhilesh and
Monika, who boosted me up in periods of mental stress & strain. It would be inappropriate if I commit a
mistake not to mention name of my juniors; Nitish, Simran, Manmeet, Nidhi, Ishita, Vibhuti and
Minakshi who kept me in exalted state during moments of despondency.
All the words in the lexicon will be futile and meaningless, if I fail to divulge my extreme sense of
regards to adorable parents (Smt. Suman Devi and Sh. Ramesh Kumar Verma) for their sacrifice, prayers
and blessings without which this work would have been a sweet dream. I owe everything to them. I am yet
to find suitable words to express my heartiest affection for my in-laws (Smt. Veena Devi and Sh. Sakreem
Chand Pathania), brothers (Rahul, Rohit and Sandeep) and my cousins (Sumit, Indu, Rahul and Nisha) for
their tender attachment and constant encouragement during the course of investigation.
I owe thanks to a very special person, my husband Dr. Mandeep Pathania for his continued and
unfailing love, support and understanding during my pursuit of Ph.D degree that made the completion of
thesis possible. You were always around at times I thought that it is impossible to continue, you helped me
to keep things in perspective. I greatly value his contribution and deeply appreciate his belief in me. I am
truly thankful for having you in my life.
I am also thankful to Mr. Ajay Walia for giving final shape to my thesis.
Place: Palampur
Dated: th February, 2017 (Arti Verma)
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TABLE OF CONTENTS

Chapter Title Page
1. INTRODUCTION 1-5
2. REVIEW OF LITERATURE 6-19
3. MATERIALS AND METHODS 20-43
4. RESULTS AND DISCUSSION 44-120
5. SUMMARY AND CONCLUSIONS 121-125
LITERATURE CITED 126-138
APPENDICES 139-150
BRIEF BIODATA OF THE STUDENT

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LIST OF ABBREVIATIONS USED
Sr. No. Abbreviation Meaning
1 et al. et alii (and other)
2 i.e. id est (that is)
3 viz., vi delicet (namely)
4 p page
5 pp particular pages
6 oC degree Celsius
7 g gram
8 / per
9 % per cent
10 > more than
11 < less than
12 m meter
13 ha hectare
14 cm centimeter
15 ml milliliter
16 mm millimeter
17 df degree of freedom
18 F first filial
1
19 @ at the rate
20 vs against
21 ppm parts per million
22 a.m.s.l above mean sea level
23 mg milligram
24 Fig. figure
25 kg kilograms
26 o degrees
27 χ2 chi-square
28 e.g. for example
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LIST OF TABLES
Table No. Title Page
3.1 List of parents and their sources involved in the study 23
4.1 Estimates of scaling tests and genic effects with respect to 46
different crosses studied for days to curd initiation
4.2 Estimates of scaling tests and genic effects with respect to 48
different crosses studied for days to marketable curd maturity
4.3 Estimates of scaling tests and genic effects with respect to 50
different crosses studied for plant frame (cm)
4.4 Estimates of scaling tests and genic effects with respect to 52
different crosses studied for plant height (cm)
4.5 Estimates of scaling tests and genic effects with respect to 53
different crosses studied for gross curd weight (g)
4.6 Estimates of scaling tests and genic effects with respect to 55
different crosses studied for marketable yield per plant (g)
4.7 Estimates of scaling tests and genic effects with respect to 57
different crosses studied for net curd weight (g)
4.8 Estimates of scaling tests and genic effects with respect to 59
different crosses studied for curd depth (cm)
4.9 Estimates of scaling tests and genic effects with respect to 61
different crosses studied for curd diameter (cm)
4.10 Estimates of scaling tests and genic effects with respect to 63
different crosses studied for curd size index (cm2)
4.11 Estimates of scaling tests and genic effects with respect to 65
different crosses studied for curd compactness (degree)
4.12 Estimates of scaling tests and genic effects with respect to 67
different crosses studied for stalk length (cm)
4.13 Estimates of scaling tests and genic effects with respect to 68
different crosses studied for number of leaves per plant
4.14 Estimates of scaling tests and genic effects with respect to 70
different crosses studied for leaf size index (cm2)
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Table No. Title Page
4.15 Estimates of scaling tests and genic effects with respect to 72
different crosses studied for per cent marketable curds (%)
4.16 Estimates of scaling tests and genic effects with respect to 74
different crosses studied for harvest index (%)
4.17 Estimates of scaling tests and genic effects with respect to 76
different crosses studied for dry matter content (%)
4.18 Estimates of scaling tests and genic effects with respect to 78
different crosses studied for total soluble solids (oBrix)
4.19 Estimates of scaling tests and genic effects with respect to 80
different crosses studied for vitamin C (mg/100g)
4.20 Estimates of scaling tests and genic effects with respect to 81
different crosses studied for total sugars (%)
4.21 Estimates of scaling tests and genic effects with respect to 84
different crosses studied for total phenols (mg/g)
4.22 Inheritance of downy mildew resistance in cauliflower 86
4.23 Phenotypic (P) and genotypic (G) correlation coefficients 92
of different horticultural and biochemical traits with plant
disease index (PDI) in cauliflower
4.24 Direct (diagonal) and indirect effects of different 99
horticultural and biochemical traits on plant disease index
(PDI) in cauliflower
4.25 Observed heterosis over better parent and standard check 107-108
(Hybrid 626) for yield and related traits in cauliflower
4.26 Top best cross-combinations showing heterosis over better 116
parent and standard check for various traits in cauliflower
4.27 Visual observation of parents and cross combinations in 117
cauliflower

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LIST OF FIGURES

Fig. No. Title Page
3.1 Mean weekly weather conditions during the cropping 21
season

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