Perspectives on Fluorescence: A Tribute to Gregorio Weber

352 Pages·2016·9.34 MB·English

by  David M. Jameson (eds.)

Springer Series on Fluorescence 17
Series Editor: Martin Hof
David M. Jameson Editor
Perspectives
on Fluorescence
A Tribute to Gregorio Weber
17
Springer Series on Fluorescence
Methods and Applications
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Moreinformationaboutthisseriesat http://www.springer.com/series/4243
Perspectives on Fluorescence
A Tribute to Gregorio Weber
Volume Editor:
David M. Jameson
With contributions by
(cid:1) (cid:1) (cid:1) (cid:1)
L.A. Bagatolli F.J. Barrantes L. Betts P. Bianchini
(cid:1) (cid:1) (cid:1) (cid:1)
L. Brand F. Cardarelli M. Castello P.L.-G Chong
(cid:1) (cid:1) (cid:1) (cid:1)
R.N. Day A.P. Demchenko A. de Silva A. Diaspro
(cid:1) (cid:1) (cid:1) (cid:1)
E. Gratton K. Jacobson D.M. Jameson T.M. Jovin
J.R. Knutson (cid:1) L. Lanzano` (cid:1) P. Liu (cid:1) G. Marriott (cid:1)
(cid:1) (cid:1) (cid:1) (cid:1)
G.D. Reinhart M. Ridilla C.A. Royer L. Scipioni
(cid:1) (cid:1) (cid:1)
R.P. Stock N.L. Thompson H. van Amerongen
(cid:1) (cid:1) (cid:1)
A. van Hoek G. Vicidomini A.J.W.G. Visser
(cid:1)
N.V. Visser J. Xu
VolumeEditor
DavidM.Jameson
JohnA.BurnsSchoolofMedicine
UniversityofHawaiiatManoa
Honolulu
Hawaii,USA
ISSN1617-1306 ISSN1865-1313 (electronic)
SpringerSeriesonFluorescence
ISBN978-3-319-41326-6 ISBN978-3-319-41328-0 (eBook)
DOI10.1007/978-3-319-41328-0
LibraryofCongressControlNumber:2016949376
#SpringerInternationalPublishingSwitzerland2016
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Series Editor
Prof.Dr.MartinHof
AcademyofSciencesoftheCzechRepublic
J.HeyrovskyInstituteofPhysicalChemistry
DepartmentofBiophysicalChemistry
Dolejskova3
16223Prague8
CzechRepublic
[email protected]
Aims and Scope
Fluorescencespectroscopy,fluorescenceimagingandfluorescentprobesareindis-
pensible tools in numerous fields of modern medicine and science, including
molecularbiology,biophysics,biochemistry,clinicaldiagnosisandanalyticaland
environmentalchemistry.Applicationsstretchfromspectroscopyandsensortech-
nology to microscopy and imaging, to single molecule detection, to the develop-
ment of novel fluorescent probes, and to proteomics and genomics. The Springer
SeriesonFluorescenceaimsatpublishingstate-of-the-artarticlesthatcanserveas
invaluable tools for both practitioners and researchers being active in this highly
interdisciplinaryfield.Thecarefullyeditedcollectionofpapersineachvolumewill
givecontinuousinspirationfornewresearchandwillpointtoexcitingnewtrends.
.
Preface
Duringthelastfewdecades,fluorescencespectroscopyhasevolvedfromanarrow,
highly specialized technique into an important discipline widely utilized in the
biological, chemical, and physical sciences. As in all scientific disciplines, the
developmentofmodernfluorescencespectroscopyhasbenefitedfromthecontribu-
tionsofmanyindividualsfrommanycountries.However,oneindividual,Gregorio
Weber, can be singled out for his outstanding and far-reaching contributions to
thisfield.
GregorioWeberwasborninArgentinaonJuly4,1916.Hediedofleukemiaon
July 18, 1996. His death ended a remarkable and amazingly productive scientific
career, which began in Buenos Aires, developed in England at Cambridge and
Sheffield, and flourished at the University of Illinois at Urbana-Champaign. His
contributions to the fields of fluorescence spectroscopy and protein chemistry are
still evident and significant yet many young people entering these fields may not
realize the debt they owe to his pioneering efforts. This book is intended to
recognize the 100th anniversary of his birth.This project began several years ago
whenIwasapproachedbyMartinHofandOttoWolfbeistoorganizethisvolume.
Tothisend,Iinvitedanumberofdistinguishedresearcherstotaketimeawayfrom
their already busy schedules and write a chapter outlining a particular aspect of
fluorescencespectroscopy,indicatinghowGregorioWeberhadinfluencedthefield
and their own approach to the work. Many of these authors had worked directly
with Gregorio Weber, either as students, postdocs, or scientists visiting his lab. I
believe that these collected chapters will not only offer the reader valuable and
informative insights into the application of fluorescence methodologies to a wide
varietyofsystemsbutwillalsoservetoemphasizethedebtthatallofusworking
withfluorescenceowetoGregorioWeber.
The first four chapters (Jameson,Barrantes, Jovin, Visser) focus largely on the
life and science of Gregorio Weber. Jameson summarizes and recounts Weber’s
scientificcareerpointingouthiscontributionstofluorescencespectroscopyaswell
as to protein chemistry. Barrantes provides a marvelously detailed look into
vii
viii Preface
Weber’s formative years in Argentina – before he left for England. Jovin follows
Weber’slifefromchildhoodtoscientificeminence,discussingmanyofthemajor
personalities and influences along the way. Visser gives a personal account of his
timeasapostdocatUIUCinWeber’slabandhisworkthereontheapplicationof
highpressuretoflavinyltryptophancompoundsandflavodoxinproteins.
Severalchaptersfocusonspectroscopy,inparticulartheapplicationoffluores-
cencespectroscopytobiophysicalsubjects.Grattonpresentsacompellingpersonal
account of the development of frequency domain fluorometry and the pivotal
influence Gregorio Weber had on his approach to this research. Visser and his
co-authors discuss the ultrafast decay of fluorescence anisotropy of NATA, while
Demchenko gives an extensive and detailed account of Weber’s red-edge effect
anditssignificancetofluorescencespectroscopyingeneralandtoproteindynamics
in particular. Day discusses modern approaches to fluorescent lifetime imaging,
while Xu and Knutson discuss the impact of laser developments on fluorescence
spectroscopy.
Two chapters concern applications of fluorescence probes to study cell mem-
branes as well as cellular interiors. Chong describes the use of fluorescence to
elucidatemembranelateralorganization,whileBagatolliandStockapply6-acyl-2-
(dimethylamino)naphthalenes as relaxation probes of biological environments to
elucidateaspectsofwaterdynamicsincellularinteriors.
Four chapters focus on proteins, in and out of cells. Reinhart presents an
engaging discussion of his early connections to the Weber lab and how Weber’s
work on the thermodynamics of protein interactions inspired his own studies on
allostericenzymes.Royerdescribeshowfluorescencecanbeappliedtocharacter-
ize the molecular and energetic basis for the role of protein interactions in the
regulationofgeneexpression.Brandprovidesadetailedexaminationofrelaxation
processes, such as time-dependent spectral shifts, exhibited by solvatochromic
probes including tryptophan, and how these processes can illuminate aspects of
proteindynamics.Marriottdescribesanewclassofgeneticallyencodedfluorescent
proteins based on the lumazine-binding protein (LUMP) and then discusses the
potentialofusingLUMPandrelatedencodedproteinstoadvancetheapplicationof
fluorescence polarization to analyze target proteins and protein interactions in
livingcells.
Several chapters describe the use of fluorescence methodologies to elucidate
aspects of cellular dynamics. Cardarelli and Gratton discuss spatiotemporal fluo-
rescence correlation spectroscopy to follow movement of single molecules inside
cells, while Diaspro and colleagues describe the use of STED microscopy to
elucidate pico-nanosecond temporal dynamics in cells. Jacobson and colleagues
discussplasmamembraneDC-SIGNclustersandtheirsignificance.
I hope you enjoy this overview of modern applications of fluorescence, and I
hopeyougainabetterappreciationnotonlyofGregorioWeber’scontributionsto
thefieldbutalsoofhisuniquepersonalityandcharacter.
Kailua,HI,USA DavidM.Jameson
Contents
AFluorescentLifetime:ReminiscingAboutGregorioWeber .............. 1
DavidM.Jameson
GregorioWeber’sRootsinArgentina ....................................... 17
FranciscoJ.Barrantes
TheLabyrinthineWorldofGregorioWeber ................................ 41
ThomasM.Jovin
PersonalRecollectionsofGregorioWeber,MyPostdocAdvisor,
andtheImportantConsequencesforMyOwnAcademicCareer ......... 57
AntonieJ.W.G.Visser
MeasurementsofFluorescenceDecayTimebytheFrequency
DomainMethod ................................................................ 67
EnricoGratton
Ultra-FastFluorescenceAnisotropyDecayof
N-Acetyl-L-TryptophanamideReportsontheApparentMicroscopic
ViscosityofAqueousSolutionsofGuanidineHydrochloride .............. 81
AntonieJ.W.G.Visser,NinaV.Visser,ArievanHoek,
andHerbertvanAmerongen
Weber’sRed-EdgeEffectthatChangedtheParadigm
inPhotophysicsandPhotochemistry ......................................... 95
AlexanderP.Demchenko
ImagingLifetimes ............................................................. 143
RichardN.Day
ix